##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547429_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	944183
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17600507528731	31.0	31.0	33.0	30.0	34.0
2	31.572181452112567	31.0	31.0	34.0	30.0	34.0
3	31.654985315346707	31.0	31.0	34.0	30.0	34.0
4	35.44197046547121	37.0	35.0	37.0	33.0	37.0
5	35.30344117612793	37.0	35.0	37.0	33.0	37.0
6	35.3704546682158	37.0	35.0	37.0	33.0	37.0
7	35.82982112577752	37.0	35.0	37.0	35.0	37.0
8	35.870728449887366	37.0	35.0	37.0	35.0	37.0
9	37.61598969691257	39.0	37.0	39.0	35.0	39.0
10	36.890705509419256	39.0	37.0	39.0	32.0	39.0
11	36.554836297624504	39.0	35.0	39.0	32.0	39.0
12	35.75267930051695	37.0	35.0	39.0	31.0	39.0
13	35.368643578628294	37.0	35.0	39.0	30.0	39.0
14	36.421846188715534	38.0	35.0	41.0	31.0	41.0
15	36.73287275877664	38.0	35.0	41.0	31.0	41.0
16	36.8764476801637	38.0	35.0	41.0	32.0	41.0
17	36.81779591456317	38.0	35.0	41.0	32.0	41.0
18	36.73136034010356	38.0	35.0	40.0	31.0	41.0
19	36.601280683935215	38.0	35.0	40.0	31.0	41.0
20	36.41823777805786	38.0	35.0	40.0	31.0	41.0
21	36.28855952712557	38.0	35.0	40.0	31.0	41.0
22	36.17871959143513	37.0	35.0	40.0	30.0	41.0
23	36.13083162903801	37.0	35.0	40.0	31.0	41.0
24	36.001600325360656	37.0	35.0	40.0	30.0	41.0
25	35.847676774523585	37.0	34.0	40.0	30.0	41.0
26	35.66957994371854	36.0	34.0	40.0	30.0	41.0
27	35.55703608304746	36.0	34.0	40.0	30.0	41.0
28	35.49523450432808	36.0	34.0	40.0	30.0	41.0
29	35.488978301875804	36.0	34.0	40.0	30.0	41.0
30	35.423008039755004	36.0	34.0	40.0	29.0	41.0
31	35.27221629705259	36.0	34.0	40.0	29.0	41.0
32	35.06541952142752	36.0	34.0	40.0	28.0	41.0
33	34.94204619231653	36.0	34.0	40.0	27.0	41.0
34	34.81644554074793	36.0	34.0	40.0	26.0	41.0
35	34.67357281374479	35.0	34.0	40.0	26.0	41.0
36	34.55755081377233	35.0	33.0	40.0	25.0	41.0
37	34.4652307868284	35.0	33.0	40.0	24.0	41.0
38	34.39695906407974	35.0	33.0	40.0	24.0	41.0
39	34.3571203887382	35.0	33.0	40.0	24.0	41.0
40	34.22710745692307	35.0	33.0	40.0	23.0	41.0
41	34.1697351043177	35.0	33.0	40.0	23.0	41.0
42	34.11054954389139	35.0	33.0	40.0	23.0	41.0
43	34.0357811991955	35.0	33.0	40.0	23.0	41.0
44	33.89177415818755	35.0	33.0	40.0	23.0	41.0
45	33.74131497813453	35.0	33.0	40.0	23.0	41.0
46	33.70388155685921	35.0	33.0	39.0	23.0	41.0
47	33.64469175996602	35.0	33.0	39.0	23.0	41.0
48	33.56606187571689	35.0	33.0	39.0	23.0	41.0
49	33.52021165388489	35.0	33.0	39.0	23.0	41.0
50	33.341425338096535	35.0	33.0	39.0	23.0	41.0
51	32.7941214785693	35.0	32.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	1.0
13	3.0
14	10.0
15	25.0
16	86.0
17	216.0
18	553.0
19	1065.0
20	1876.0
21	2938.0
22	4442.0
23	6508.0
24	9529.0
25	13204.0
26	16453.0
27	19116.0
28	21080.0
29	24300.0
30	28354.0
31	34211.0
32	41939.0
33	53877.0
34	89609.0
35	137869.0
36	82018.0
37	81131.0
38	106435.0
39	167223.0
40	108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.26618462734449	25.373682855971776	28.337409167502486	15.02272334918125
2	31.33492130233228	26.089857580574954	29.340604522640206	13.23461659445256
3	27.232009049093236	26.41903105647952	32.840773451756704	13.508186442670542
4	24.947812023728453	28.34471707285558	32.64960288418665	14.057868019229321
5	22.750674392570083	32.95145114877095	31.079991908348276	13.217882550310692
6	19.69120392974667	41.52055268946804	28.064686612658775	10.723556768126517
7	86.07070875031641	4.0580057044026425	8.025139194414642	1.8461463508663047
8	86.53629645947872	3.489048203579179	7.448767876566301	2.5258874603757957
9	82.05072533608421	5.659919740135122	9.522942056783485	2.766412866997182
10	47.785757633848526	25.9779089434993	14.564019898684894	11.672313523967281
11	39.57114245861237	23.592778094924395	21.90602881009296	14.930050636370279
12	36.11217316981983	24.39145801184728	24.915932610521477	14.58043620781141
13	19.95672449090907	39.94236286821516	25.547801644384617	14.553110996491148
14	14.75910919811096	42.35736080823315	27.963752789448655	14.919777204207236
15	12.979793112140337	27.775018190329632	44.355702231453016	14.88948646607702
16	14.841190743743532	23.61565501602973	43.491357078024066	18.051797162202664
17	14.587850024836287	25.977485296812162	28.01342536351534	31.421239314836214
18	19.369762005882336	28.19146288378418	32.63096242995267	19.807812680380817
19	25.071093209684985	30.101050326049084	25.047157171861812	19.780699292404123
20	27.21029715637752	28.19389885223521	24.645222377441662	19.9505816139456
21	19.7603642514216	33.402105312211724	27.60195851863463	19.23557191773205
22	21.37297536600426	29.202495702633918	25.078189291694514	24.34633963966731
23	17.35458062684882	34.34005907753052	24.844124497051947	23.461235798568712
24	18.005619673304857	29.02075127385263	36.231111977233226	16.742517075609285
25	16.369390255914375	30.361804861981206	33.5350244603006	19.73378042180382
26	15.58945670489725	37.54251029726229	27.070705572966258	19.797327424874204
27	16.61817677293491	37.14163461955998	27.603547193711385	18.636641413793726
28	14.903149071737154	32.93609395636227	34.70270064171882	17.458056330181755
29	15.944048982029969	28.876923223570007	33.90921039671335	21.269817397686676
30	18.423864865179738	34.048484245109265	29.09086480057362	18.43678608913738
31	26.40706303756793	30.838195561665483	23.319843716737115	19.434897684029472
32	27.602276253649983	30.979163996809937	24.7744346170181	16.644125132521978
33	24.455746396620146	31.22869189553296	23.9117840503377	20.40377765750919
34	19.269675476046487	31.350702141428094	26.913850387054204	22.46577199547122
35	18.259595862242804	30.36614724052435	30.091412363916742	21.282844533316105
36	25.96636457127485	30.74192185201386	25.00521614983536	18.286497426875933
37	18.21744301687279	36.060064627302125	28.06934672621727	17.65314562960782
38	18.116403281990888	36.801022683102744	24.69680136160045	20.385772673305915
39	17.634928822060978	35.95510616056421	26.29066611027735	20.11929890709746
40	21.77872298060863	30.756431751048265	25.764179189839258	21.70066607850385
41	15.783169152590123	29.862431329519808	27.286977206749114	27.067422311140955
42	20.259420048867646	31.3935963685006	24.27495517288492	24.07202840974684
43	20.653623291247566	31.12712260229214	25.797223631435855	22.42203047502444
44	18.893900864556976	34.08767156366933	27.074094746463345	19.944332825310347
45	16.709366722340903	38.68021347556565	23.148902278477795	21.461517523615655
46	21.052168912170625	34.86114450270763	25.869137656577166	18.217548928544574
47	20.909717713621195	31.57311665217442	25.614843732623864	21.90232190158052
48	20.269587569358908	29.71595548744258	29.309254667792157	20.705202275406357
49	19.744159765638653	28.28784250510759	30.125727745574743	21.84226998367901
50	17.519379188144672	34.76518852807136	27.701515490111557	20.01391679367241
51	17.013121397017315	35.16129818054339	25.806649770224627	22.018930652214667
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2478.0
1	2129.5
2	1781.0
3	8836.0
4	15891.0
5	10132.0
6	4373.0
7	5470.5
8	6568.0
9	8690.5
10	10813.0
11	12317.0
12	13821.0
13	13899.0
14	13977.0
15	12620.0
16	11263.0
17	9968.5
18	8674.0
19	7708.5
20	6743.0
21	6342.5
22	5942.0
23	6223.0
24	6504.0
25	6457.5
26	6707.0
27	7003.0
28	8366.5
29	9730.0
30	12781.0
31	15832.0
32	17019.0
33	18206.0
34	21078.5
35	23951.0
36	25208.0
37	26465.0
38	30935.5
39	35406.0
40	46888.0
41	58370.0
42	68881.5
43	79393.0
44	86183.0
45	92973.0
46	92553.5
47	92134.0
48	90272.0
49	88410.0
50	79442.0
51	70474.0
52	62498.5
53	54523.0
54	47842.5
55	41162.0
56	35855.0
57	30548.0
58	27486.5
59	24425.0
60	21714.0
61	19003.0
62	15694.0
63	12385.0
64	10708.5
65	9032.0
66	7861.5
67	6691.0
68	5711.0
69	4731.0
70	4092.0
71	3453.0
72	2603.0
73	1753.0
74	1423.0
75	982.0
76	871.0
77	702.5
78	534.0
79	375.0
80	216.0
81	142.0
82	68.0
83	50.5
84	33.0
85	31.5
86	30.0
87	21.5
88	13.0
89	12.0
90	11.0
91	8.5
92	6.0
93	11.0
94	16.0
95	8.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	944183.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.160861185286116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.66307845703952	17.75582918180191
2	8.373858541691108	3.8789155053866033
3	3.528644909335372	2.451793647518492
4	1.9799729226291456	1.8343151204655554
5	1.2712408665070551	1.4721516621116375
6	0.8356764374148413	1.1612991577667748
7	0.600513472698976	0.9735886426752561
8	0.44364885708516677	0.8220231675168308
9	0.3446535760796908	0.718422626933475
>10	3.708944529077238	20.139921346587478
>50	1.6150281184483521	26.8508160959238
>100	0.6264239498276605	19.001343996356454
>500	0.006929468471544916	1.045982842083105
>1k	9.239291295393222E-4	0.4736667391470853
>5k	0.0	0.0
>10k+	4.619645647696611E-4	1.4199302677255408
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13271	1.4055537962450075	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2257	0.23904264321641036	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2170	0.22982832777120538	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0591167178396561E-4	0.0	0.0	0.0902367443599387	0.0
2	1.0591167178396561E-4	0.0	0.0	0.3020600879278699	0.0
3	1.0591167178396561E-4	0.0	0.0	0.49037104035976076	0.0
4	1.0591167178396561E-4	0.0	0.0	0.8091651724294973	0.0
5	1.0591167178396561E-4	0.0	0.0	1.4053419729014396	0.0
6	1.0591167178396561E-4	0.0	0.0	2.3962515741122217	0.0
7	1.0591167178396561E-4	0.0	0.0	2.98056626734436	0.0
8	1.0591167178396561E-4	0.0	0.0	4.135427136476721	0.0
9	1.0591167178396561E-4	0.0	0.0	4.6333179055331435	0.0
10	1.0591167178396561E-4	0.0	0.0	5.253960302187182	0.0
11	1.0591167178396561E-4	0.0	0.0	5.9235338912054125	0.0
12	1.0591167178396561E-4	0.0	0.0	6.4044787927764	0.0
13	1.0591167178396561E-4	0.0	0.0	6.633671650516902	0.0
14	1.0591167178396561E-4	0.0	0.0	6.734393650383454	0.0
15	1.0591167178396561E-4	0.0	0.0	6.8526969877661426	0.0
16	1.0591167178396561E-4	0.0	0.0	7.102436709832734	0.0
17	1.0591167178396561E-4	0.0	0.0	7.376536116409636	0.0
18	1.0591167178396561E-4	0.0	0.0	7.728056955060619	0.0
19	1.0591167178396561E-4	0.0	0.0	7.8952914848075	0.0
20	3.177350153518968E-4	0.0	0.0	8.058925017713728	0.0
21	3.177350153518968E-4	0.0	0.0	8.293095724028076	0.0
22	3.177350153518968E-4	0.0	0.0	8.5122269729491	0.0
23	3.177350153518968E-4	0.0	0.0	8.763555370092451	0.0
24	3.177350153518968E-4	0.0	0.0	8.96171610800025	0.0
25	3.177350153518968E-4	0.0	0.0	9.120265880660847	0.0
26	3.177350153518968E-4	0.0	0.0	9.261234315805305	0.0
27	3.177350153518968E-4	0.0	0.0	9.399660870826947	0.0
28	3.177350153518968E-4	0.0	0.0	9.551855943180506	0.0
29	3.177350153518968E-4	0.0	0.0	9.7071224540158	0.0
30	3.177350153518968E-4	0.0	0.0	9.929536964762129	0.0
31	3.177350153518968E-4	0.0	0.0	10.1146705670405	0.0
32	3.177350153518968E-4	0.0	0.0	10.274279456418936	0.0
33	3.177350153518968E-4	0.0	0.0	10.428169115521039	0.0
34	3.177350153518968E-4	0.0	0.0	10.596886408672894	0.0
35	3.177350153518968E-4	0.0	0.0	10.811357544035426	0.0
36	3.177350153518968E-4	0.0	0.0	10.983781745699721	0.0
37	3.177350153518968E-4	0.0	0.0	11.159595120861104	0.0
38	3.177350153518968E-4	0.0	0.0	11.325982357233714	0.0
39	3.177350153518968E-4	0.0	0.0	11.499147940600498	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	35	1.2116107E-7	45.000004	1
TCGATAG	55	1.8189894E-12	45.000004	1
GTTACAT	35	1.2116107E-7	45.000004	32
AATAGTC	35	1.2116107E-7	45.000004	34
CGAACGA	25	3.890275E-5	45.0	18
GATCGTC	20	7.032646E-4	45.0	9
TCGATTG	25	3.890275E-5	45.0	1
CGACACC	20	7.032646E-4	45.0	17
TCGATAA	20	7.032646E-4	45.0	14
CGTTCCG	25	3.890275E-5	45.0	14
CCGTCAC	20	7.032646E-4	45.0	32
ATCCGTC	40	6.8121153E-9	45.0	28
TACGCGC	20	7.032646E-4	45.0	34
CTACGAT	50	2.1827873E-11	45.0	42
TACGAAG	50	2.1827873E-11	45.0	1
CCGAGTT	20	7.032646E-4	45.0	41
GTCGTCA	20	7.032646E-4	45.0	9
GGTATCG	25	3.890275E-5	45.0	1
CGTAATT	20	7.032646E-4	45.0	39
CCGGTCA	20	7.032646E-4	45.0	31
>>END_MODULE