FastQCFastQC Report
Thu 26 May 2016
SRR1547428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547428_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3378991
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT582681.7244201005566455No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71610.21192716997470548No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC66010.19535417525527593No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT49950.14782519397062616No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCT41210.12195948435494502No Hit
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG34770.10290054042760102TruSeq Adapter, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGA207.0352305E-445.00000426
TCGGATC207.0352305E-445.00000423
GCGTATT207.0352305E-445.00000431
CGTGACG351.2128658E-745.019
CGCGACT253.892418E-545.017
CTACGCG253.892418E-545.01
AGCGCGT351.2128658E-745.024
CCAACGA600.044.99999623
TCGAATT600.044.99999634
TACGTCA302.1667583E-644.99999611
CGTTTTT259750.043.7439841
TATTACG1100.042.9545441
CTAAGCG900.042.5000041
CGGTCTA7250.041.5862131
CGACGGT7300.041.30137328
TGCCGAT556.184564E-1140.90909221
CTGTCGG2750.040.909092
CCAATCG3250.040.84615324
CACGACG7500.040.826
ACGGGTA3750.040.85
Produced by FastQC (version 0.11.5)