##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547428_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3378991
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21379843864633	31.0	31.0	33.0	30.0	34.0
2	31.596509431365753	31.0	31.0	34.0	30.0	34.0
3	31.600768099115978	31.0	31.0	34.0	30.0	34.0
4	35.43876352437754	37.0	35.0	37.0	33.0	37.0
5	35.335481805071396	37.0	35.0	37.0	33.0	37.0
6	35.39248580419421	37.0	35.0	37.0	33.0	37.0
7	35.880132856228386	37.0	35.0	37.0	35.0	37.0
8	35.929581937329814	37.0	35.0	37.0	35.0	37.0
9	37.66651464889963	39.0	37.0	39.0	35.0	39.0
10	36.9177872921236	39.0	37.0	39.0	32.0	39.0
11	36.62577615625493	39.0	35.0	39.0	32.0	39.0
12	36.03719867854043	37.0	35.0	39.0	31.0	39.0
13	35.79549250057192	38.0	35.0	39.0	31.0	39.0
14	36.89290501217671	39.0	35.0	41.0	31.0	41.0
15	37.108744296744206	39.0	35.0	41.0	32.0	41.0
16	37.20828850979479	39.0	35.0	41.0	32.0	41.0
17	37.152858945170316	39.0	35.0	41.0	32.0	41.0
18	37.00107043789107	38.0	35.0	40.0	32.0	41.0
19	36.8323324921552	38.0	35.0	40.0	32.0	41.0
20	36.613961090751644	38.0	35.0	40.0	31.0	41.0
21	36.513020011003285	38.0	35.0	40.0	31.0	41.0
22	36.40884512566029	38.0	35.0	40.0	31.0	41.0
23	36.3483711557681	38.0	35.0	40.0	31.0	41.0
24	36.24627233396005	38.0	35.0	40.0	31.0	41.0
25	36.12498731130092	37.0	35.0	40.0	31.0	41.0
26	35.95806618011116	37.0	35.0	40.0	30.0	41.0
27	35.854244358744964	37.0	35.0	40.0	30.0	41.0
28	35.76134710036221	37.0	35.0	40.0	30.0	41.0
29	35.74649118627425	37.0	35.0	40.0	30.0	41.0
30	35.64560041740271	37.0	34.0	40.0	30.0	41.0
31	35.461661188206776	37.0	34.0	40.0	29.0	41.0
32	35.20205410431694	37.0	34.0	40.0	27.0	41.0
33	34.964890406633224	37.0	34.0	40.0	26.0	41.0
34	34.72411705151035	37.0	34.0	40.0	24.0	41.0
35	34.50718927632539	37.0	34.0	40.0	24.0	41.0
36	34.37747363044175	36.0	33.0	40.0	23.0	41.0
37	34.294333426753724	36.0	33.0	40.0	23.0	41.0
38	34.19935596158735	36.0	33.0	40.0	23.0	41.0
39	34.14543069218	36.0	33.0	40.0	23.0	41.0
40	34.05125464968685	36.0	33.0	40.0	22.0	41.0
41	33.97591085622898	36.0	33.0	40.0	22.0	41.0
42	33.913947092490034	36.0	33.0	40.0	21.0	41.0
43	33.86573832247556	36.0	33.0	40.0	22.0	41.0
44	33.71965536457481	35.0	33.0	40.0	21.0	41.0
45	33.57534867657238	35.0	33.0	40.0	20.0	41.0
46	33.53044237170209	35.0	33.0	40.0	20.0	41.0
47	33.47679973104397	35.0	33.0	40.0	20.0	41.0
48	33.37859141974631	35.0	33.0	40.0	20.0	41.0
49	33.32901567361381	35.0	33.0	40.0	20.0	41.0
50	33.19318784808838	35.0	33.0	40.0	20.0	41.0
51	32.666057115866835	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	5.0
13	16.0
14	29.0
15	107.0
16	303.0
17	724.0
18	1792.0
19	3671.0
20	6409.0
21	10564.0
22	16585.0
23	25092.0
24	38081.0
25	55323.0
26	70311.0
27	75271.0
28	77034.0
29	83065.0
30	95822.0
31	114677.0
32	141002.0
33	180097.0
34	286409.0
35	435097.0
36	292560.0
37	306735.0
38	408854.0
39	652933.0
40	419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.12565259866037	23.65726928541686	28.18909550217802	17.027982613744754
2	34.59023714475712	24.98529886584486	28.05565330005318	12.368810689344837
3	26.28956395563054	24.80116105665863	36.20231601682277	12.706958970888055
4	23.68189202042858	27.450975749861424	35.324095269860145	13.54303695984985
5	21.693990898466435	30.678536876836905	34.21400056999264	13.413471654704024
6	18.852994873321652	39.875128403715784	30.99685675398366	10.275019968978905
7	82.55269102522024	4.381248721881769	11.148771926293973	1.917288326604007
8	83.15023626875598	3.2996240593715696	10.606391079467214	2.9437485924052478
9	78.64963239026088	5.214130490433387	13.07452431805826	3.061712801247473
10	43.00703967545341	22.237348368196304	20.72346448984327	14.032147466507014
11	34.64063680548424	24.655496270928214	25.90323561086727	14.800631312720277
12	30.677737821734357	24.148096280812823	28.862846926789683	16.311318970663137
13	19.28318246482456	35.45398611597367	30.73624641202063	14.526585007181136
14	16.06115553430003	37.64253885257463	31.403753368979082	14.892552244146257
15	14.133834626964084	28.129610288988637	43.40212803171124	14.334427052336038
16	15.079057624006692	24.685505229223754	42.42381823449663	17.811618912272927
17	15.824457656146466	26.43380819895643	30.492357037944167	27.24937710695293
18	18.65334947621938	27.730289900150666	34.664756431727696	18.95160419190226
19	23.360908626273346	30.435180206162137	28.47284884748139	17.731062320083126
20	25.355468540756693	28.09954214142624	28.07119048260265	18.473798835214417
21	19.997774483566246	31.914823093639495	30.120914793794952	17.96648762899931
22	20.515266243680436	27.975481438097944	28.093268079139598	23.41598423908202
23	17.6168566296862	32.76572799394849	27.869710218227866	21.747705158137446
24	17.35065290200536	29.280308825918745	36.24886837520431	17.12016989687158
25	17.222833680231762	30.003068963486434	34.02767275793277	18.746424598349034
26	15.946742681469114	36.23404146385711	29.2267721340483	18.592443720625475
27	16.879831878806424	35.15543545395652	29.749324576478596	18.21540809075845
28	15.926174411236966	31.718285133047114	35.67638978618174	16.67915066953419
29	15.804066953714882	28.970038689064282	35.24635016784596	19.979544189374877
30	17.270244282982702	33.2776559629783	31.79064401177748	17.661455742261523
31	22.62489009293011	31.528642722043354	27.61729758972427	18.229169595302267
32	23.585887029589603	30.971642126303383	28.892204803149816	16.550266040957197
33	22.726251712419476	32.61026738455355	26.495187468685177	18.16829343434179
34	18.21730214729782	32.266052203157685	28.86252138582198	20.654124263722515
35	18.296645359517086	31.674751427275183	29.926655619976493	20.101947593231234
36	24.572364945630216	30.834471000366676	27.14905129963353	17.444112754369574
37	19.23609740304132	34.30515204094951	28.6444977213612	17.814252834647977
38	18.337012439512268	36.46834217670305	25.551148256979673	19.643497126805013
39	18.655480289826166	33.7049432804053	27.680245375024676	19.959331054743856
40	21.540779481211995	30.7237574767142	27.05053076495321	20.684932277120595
41	16.62061248461449	29.44364160780541	28.88817993300367	25.047565974576436
42	19.347817144230333	31.1828294304424	26.643545366057502	22.825808059269765
43	19.0653955574312	30.48969352093569	28.512831197242015	21.932079724391098
44	18.53834473071991	33.50417328723279	27.89797309315118	20.059508888896126
45	17.45248803562957	36.52223400417462	25.635788908582473	20.389489051613335
46	20.80331081083081	33.731903991457806	26.330522928294275	19.134262269417114
47	19.662378502931794	31.564688985558114	27.38956688549925	21.383365626010843
48	19.484662729199336	29.93668820070844	29.80771478823116	20.770934281861063
49	19.63485549384417	28.467018704696166	30.088508670191782	21.809617131267885
50	18.227956215331737	32.58901843775257	28.412712552356606	20.770312794559086
51	16.97820444031961	33.912460849999306	26.43215089948449	22.677183810196595
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8320.0
1	7329.5
2	6339.0
3	39908.5
4	73478.0
5	48691.0
6	23904.0
7	26416.5
8	28929.0
9	34104.5
10	39280.0
11	43528.5
12	47777.0
13	48616.5
14	49456.0
15	46002.0
16	42548.0
17	39302.5
18	36057.0
19	33223.0
20	30389.0
21	29846.0
22	29303.0
23	28687.0
24	28071.0
25	28070.0
26	31841.5
27	35614.0
28	39016.5
29	42419.0
30	48164.0
31	53909.0
32	59868.0
33	65827.0
34	78815.5
35	91804.0
36	99688.0
37	107572.0
38	123498.5
39	139425.0
40	175343.5
41	211262.0
42	256402.5
43	301543.0
44	328215.0
45	354887.0
46	355047.0
47	355207.0
48	327138.0
49	299069.0
50	259040.5
51	219012.0
52	189779.5
53	160547.0
54	145003.0
55	129459.0
56	115001.0
57	100543.0
58	86404.0
59	72265.0
60	63343.5
61	54422.0
62	47278.5
63	40135.0
64	33046.5
65	25958.0
66	20058.5
67	14159.0
68	12145.0
69	10131.0
70	9363.0
71	8595.0
72	6731.5
73	4868.0
74	4386.5
75	2832.0
76	1759.0
77	1510.5
78	1262.0
79	979.0
80	696.0
81	553.5
82	411.0
83	346.5
84	282.0
85	171.0
86	60.0
87	39.0
88	18.0
89	18.0
90	18.0
91	16.5
92	15.0
93	11.0
94	7.0
95	6.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3378991.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.10485796223609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.37700229762055	16.661979294080453
2	9.71397787706485	4.294522024416443
3	3.800539728481412	2.5203117263375074
4	2.0253919114151975	1.7908400207877917
5	1.2932172915207893	1.4293192271687352
6	1.0001641962804593	1.3265092498616138
7	0.703028152922462	1.0878236224662952
8	0.5490088627110814	0.9708610344189781
9	0.4682056712070659	0.9314657873231041
>10	3.578705387930356	16.10651807876872
>50	0.5979895254762809	9.45938835917999
>100	0.8790056203237776	38.21910728110666
>500	0.010356675796875582	1.5552974709977578
>1k	0.0029979850990955632	1.4763156386272098
>5k	2.725440999177785E-4	0.4145491903445759
>10k+	1.3627204995888924E-4	1.7551919941140637
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58268	1.7244201005566455	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7161	0.21192716997470548	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6601	0.19535417525527593	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	4995	0.14782519397062616	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCT	4121	0.12195948435494502	No Hit
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG	3477	0.10290054042760102	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.9189266855105564E-5	0.0	0.0	0.10375878479700006	0.0
2	5.9189266855105564E-5	0.0	0.0	0.3227590721608906	0.0
3	5.9189266855105564E-5	0.0	0.0	0.5457546350375009	0.0
4	5.9189266855105564E-5	0.0	0.0	0.9294786520591503	0.0
5	5.9189266855105564E-5	0.0	0.0	1.5675389487571882	0.0
6	5.9189266855105564E-5	0.0	0.0	2.7945916399303816	0.0
7	5.9189266855105564E-5	0.0	0.0	3.4908941752138434	0.0
8	5.9189266855105564E-5	0.0	0.0	4.910963065601536	0.0
9	5.9189266855105564E-5	0.0	0.0	5.556510804556745	0.0
10	8.878390028265834E-5	0.0	0.0	6.483000398639712	0.0
11	8.878390028265834E-5	0.0	0.0	7.397948085685934	0.0
12	8.878390028265834E-5	0.0	0.0	8.078772627686785	0.0
13	1.1837853371021113E-4	0.0	0.0	8.470132060132745	0.0
14	1.1837853371021113E-4	0.0	0.0	8.650629729407388	0.0
15	1.1837853371021113E-4	0.0	0.0	8.828315908506415	0.0
16	1.1837853371021113E-4	0.0	0.0	9.193188144034714	0.0
17	1.1837853371021113E-4	0.0	0.0	9.604050439909429	0.0
18	1.1837853371021113E-4	0.0	0.0	10.123051526328421	0.0
19	1.1837853371021113E-4	0.0	0.0	10.410592984710524	0.0
20	1.1837853371021113E-4	0.0	0.0	10.694168762213335	0.0
21	1.1837853371021113E-4	0.0	0.0	11.088517252635476	0.0
22	1.1837853371021113E-4	0.0	0.0	11.47943276558002	0.0
23	1.1837853371021113E-4	0.0	0.0	11.959339341241217	0.0
24	1.1837853371021113E-4	0.0	0.0	12.3063660128127	0.0
25	1.1837853371021113E-4	0.0	0.0	12.593049226825404	0.0
26	1.1837853371021113E-4	0.0	0.0	12.869019183537334	0.0
27	1.1837853371021113E-4	0.0	0.0	13.135282100485027	0.0
28	1.1837853371021113E-4	0.0	0.0	13.434602222971295	0.0
29	1.1837853371021113E-4	0.0	0.0	13.766772388562147	0.0
30	1.1837853371021113E-4	0.0	0.0	14.139220850247899	0.0
31	1.1837853371021113E-4	0.0	0.0	14.475948589386595	0.0
32	1.1837853371021113E-4	0.0	0.0	14.787076970610457	0.0
33	1.1837853371021113E-4	0.0	0.0	15.108385905733398	0.0
34	1.1837853371021113E-4	0.0	0.0	15.42205942543203	0.0
35	1.1837853371021113E-4	0.0	0.0	15.765327578558214	0.0
36	1.1837853371021113E-4	0.0	0.0	16.09217071013211	0.0
37	1.1837853371021113E-4	0.0	0.0	16.424873579124657	0.0
38	1.1837853371021113E-4	0.0	0.0	16.772876873599248	0.0
39	1.1837853371021113E-4	0.0	0.0	17.108391232767413	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGA	20	7.0352305E-4	45.000004	26
TCGGATC	20	7.0352305E-4	45.000004	23
GCGTATT	20	7.0352305E-4	45.000004	31
CGTGACG	35	1.2128658E-7	45.0	19
CGCGACT	25	3.892418E-5	45.0	17
CTACGCG	25	3.892418E-5	45.0	1
AGCGCGT	35	1.2128658E-7	45.0	24
CCAACGA	60	0.0	44.999996	23
TCGAATT	60	0.0	44.999996	34
TACGTCA	30	2.1667583E-6	44.999996	11
CGTTTTT	25975	0.0	43.743984	1
TATTACG	110	0.0	42.954544	1
CTAAGCG	90	0.0	42.500004	1
CGGTCTA	725	0.0	41.58621	31
CGACGGT	730	0.0	41.301373	28
TGCCGAT	55	6.184564E-11	40.909092	21
CTGTCGG	275	0.0	40.90909	2
CCAATCG	325	0.0	40.846153	24
CACGACG	750	0.0	40.8	26
ACGGGTA	375	0.0	40.8	5
>>END_MODULE