FastQCFastQC Report
Thu 26 May 2016
SRR1547427_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547427_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1788572
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96210.537915163605379No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29370.16420921271271158No Hit
GAGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT19180.10723638746441294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG351.2124292E-745.0000041
TCGAGTA351.2124292E-745.0000041
TCCATCG253.8916798E-545.018
GGTCGAA207.03434E-445.017
CGCGGAT207.03434E-445.023
ACGTTAG253.8916798E-545.01
GCGAACG453.8562575E-1045.01
CGTTGCC253.8916798E-545.016
TAACGCG406.8193913E-945.01
CGCGAAC207.03434E-445.040
CGTTAGT207.03434E-445.031
TACGCGG1100.045.02
CGCTATA253.8916798E-545.031
CTACGAT207.03434E-445.039
CGCTAAC453.8562575E-1045.024
GTCGTAA253.8916798E-545.010
GTCCGAT253.8916798E-545.08
CGTACGT207.03434E-445.012
TCCGTCT207.03434E-445.024
TTACGGC253.8916798E-545.014