##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547427_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1788572
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.251776836493022	31.0	31.0	33.0	30.0	34.0
2	31.653364248126437	31.0	31.0	34.0	30.0	34.0
3	31.806480253520686	31.0	31.0	34.0	30.0	34.0
4	35.552097986550166	37.0	35.0	37.0	33.0	37.0
5	35.41541184811123	37.0	35.0	37.0	33.0	37.0
6	35.470321574977135	37.0	35.0	37.0	33.0	37.0
7	35.88294236966698	37.0	35.0	37.0	35.0	37.0
8	35.926855614423125	37.0	35.0	37.0	35.0	37.0
9	37.713371337581044	39.0	37.0	39.0	35.0	39.0
10	36.92745050241198	39.0	37.0	39.0	32.0	39.0
11	36.670820073220426	39.0	35.0	39.0	32.0	39.0
12	35.99569321223859	37.0	35.0	39.0	32.0	39.0
13	35.63708925332612	37.0	35.0	39.0	30.0	39.0
14	36.70459170779818	38.0	35.0	41.0	31.0	41.0
15	36.96283403743322	38.0	35.0	41.0	32.0	41.0
16	37.08557161802824	38.0	35.0	41.0	32.0	41.0
17	37.022404465685476	38.0	35.0	41.0	32.0	41.0
18	36.949753210941466	38.0	35.0	41.0	32.0	41.0
19	36.8981550644872	38.0	35.0	41.0	32.0	41.0
20	36.7552958449534	38.0	35.0	41.0	31.0	41.0
21	36.63869276719081	38.0	35.0	40.0	31.0	41.0
22	36.55162218798013	38.0	35.0	40.0	31.0	41.0
23	36.530228584591505	38.0	35.0	40.0	31.0	41.0
24	36.411948750176116	38.0	35.0	40.0	31.0	41.0
25	36.309371945887555	38.0	35.0	40.0	31.0	41.0
26	36.15568453492507	38.0	35.0	40.0	30.0	41.0
27	36.08356107553959	38.0	35.0	40.0	30.0	41.0
28	36.05142985577321	38.0	35.0	40.0	30.0	41.0
29	36.10677400742045	38.0	35.0	40.0	30.0	41.0
30	36.08644158580141	38.0	35.0	40.0	30.0	41.0
31	35.96727556956052	38.0	35.0	40.0	30.0	41.0
32	35.86668191160322	38.0	34.0	40.0	30.0	41.0
33	35.74554057650461	38.0	34.0	40.0	30.0	41.0
34	35.66052974104481	38.0	34.0	40.0	29.0	41.0
35	35.52595590225051	38.0	34.0	40.0	29.0	41.0
36	35.45403483896651	38.0	34.0	40.0	28.0	41.0
37	35.36740595290544	37.0	34.0	40.0	28.0	41.0
38	35.34047944393628	37.0	34.0	40.0	28.0	41.0
39	35.284081937992994	37.0	34.0	40.0	27.0	41.0
40	35.166135889413454	37.0	34.0	40.0	27.0	41.0
41	35.1231395772717	37.0	34.0	40.0	27.0	41.0
42	35.10012736417656	37.0	34.0	40.0	27.0	41.0
43	35.06847250208546	37.0	34.0	40.0	27.0	41.0
44	34.96885616010985	37.0	34.0	40.0	27.0	41.0
45	34.84398335655484	37.0	34.0	40.0	26.0	41.0
46	34.822752452794745	37.0	34.0	40.0	26.0	41.0
47	34.7675005535142	36.0	34.0	40.0	26.0	41.0
48	34.71597676805854	36.0	34.0	40.0	26.0	41.0
49	34.69262014612775	36.0	34.0	40.0	26.0	41.0
50	34.54737466537551	36.0	34.0	40.0	26.0	41.0
51	33.97902237091937	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	4.0
13	3.0
14	13.0
15	44.0
16	111.0
17	310.0
18	627.0
19	1334.0
20	2309.0
21	3630.0
22	5750.0
23	8954.0
24	13097.0
25	18381.0
26	24437.0
27	29425.0
28	33797.0
29	39866.0
30	48121.0
31	58844.0
32	72673.0
33	95349.0
34	158115.0
35	238429.0
36	132685.0
37	159841.0
38	234045.0
39	408150.0
40	223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.620125999959747	24.900814728174208	30.144551072028413	14.334508199837636
2	28.23543027622036	26.697779010294248	31.657266243684905	13.409524469800488
3	27.60694006168049	26.767163972152087	32.22436670148029	13.401529264687136
4	24.89237223885871	28.286308854214425	32.26529320597661	14.556025700950256
5	22.34408231818456	32.19775329145263	30.97191502494728	14.486249365415539
6	20.615943892669684	41.22769449594425	27.10022297117477	11.056138640211296
7	88.79989175722308	3.6701905207059036	5.90459875252436	1.625318969546655
8	90.10394884857864	2.3568522821558204	5.130405709135556	2.408793160129981
9	86.39534779701349	3.636308742393373	7.476467259914614	2.49187620067853
10	43.46445096982397	24.612875523042966	15.937407048751743	15.985266458381323
11	38.32828647658579	25.20071878571285	22.229913025586892	14.24108171211447
12	36.07140221360952	22.04585557640397	24.216022614689262	17.666719595297252
13	20.74397899553387	34.73782436491234	26.2969564546465	18.221240184907288
14	15.925498106869615	37.45200081405724	28.282786491122526	18.339714587950613
15	15.110210827408682	26.013769644163055	42.48953914072232	16.386480387705944
16	16.749954712474533	22.530991204156166	42.14630442610082	18.57274965726848
17	17.055393912014726	23.002931947945065	28.849383754190494	31.09229038584972
18	20.235920052421708	25.349776246077877	33.466754483465024	20.947549218035395
19	26.170039562287677	26.658641642606508	26.77912882455948	20.392189970546333
20	28.70547006215014	24.615950601932717	26.34638135898359	20.332197976933553
21	21.5981240900562	29.567889914412167	29.013481145852673	19.82050484967896
22	21.783747033946636	25.35218039866441	27.353329919063924	25.51074264832503
23	20.309833766826273	29.953001612459552	26.60608574885439	23.131078871859785
24	19.81178280773712	24.901821117629037	36.37225675007771	18.914139324556125
25	18.673668155377587	25.492292174986524	33.9462990586904	21.887740610945492
26	17.38258230588425	34.38497303994472	27.506636579349337	20.7258080748217
27	17.553444871103874	33.86528470757677	28.679751220526768	19.90151920079259
28	15.863549244872447	29.969327485837866	35.687408726067495	18.47971454322219
29	16.511943606407794	26.18569450936278	35.057185285244316	22.24517659898511
30	18.867789499108785	30.115030314686802	30.37221873092053	20.64496145528388
31	25.182771507101755	28.703625014816293	25.732483791538723	20.38111968654323
32	27.301612683190836	27.330071140552352	26.15388142048517	19.21443475577164
33	25.830159479182274	28.206300892555625	25.757363975283077	20.206175652979024
34	20.220880121124562	28.090845657876784	27.909192361280393	23.779081859718257
35	20.08322840791425	26.17719610952201	30.674247388419364	23.06532809414438
36	26.768394003707986	26.40329827370662	27.496516774275793	19.331790948309603
37	20.020049514361176	30.473025407979105	30.25279384894765	19.25413122871207
38	21.608243895129746	30.47979058153655	26.033673791158535	21.878291732175164
39	20.663747391773995	28.973728762386976	29.434599222172775	20.927924623666254
40	23.351310430891235	26.19922485647768	28.495022845040623	21.954441867590457
41	17.847366502438817	25.553570110680475	30.377474320295743	26.22158906658496
42	22.438179732210948	26.535638487016456	27.022675072627774	24.003506708144823
43	22.04518465010075	26.282475628602036	28.253153912730376	23.419185808566834
44	20.728212227408235	29.63134836059158	27.805366515857344	21.835072896142847
45	19.08013767407742	32.753336181042755	25.316453573018027	22.850072571861798
46	22.311933766155345	30.2303737283151	26.67256336339829	20.785129142131265
47	21.03258912696833	27.890853708992424	27.797259489693456	23.27929767434579
48	21.729961108638623	25.370239498326043	30.773768123396767	22.12603126963857
49	21.342221615903636	24.870175760327236	31.12237024844401	22.66523237532512
50	19.816367470809116	29.84034190404412	28.700661756977077	21.64262886816969
51	18.55871611542616	30.97208275652308	26.309424501781308	24.15977662626945
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2081.0
1	2315.0
2	2549.0
3	8238.5
4	13928.0
5	9539.5
6	5151.0
7	5623.5
8	6096.0
9	6895.5
10	7695.0
11	8166.0
12	8637.0
13	8539.5
14	8442.0
15	7852.0
16	7262.0
17	7194.5
18	7127.0
19	6802.0
20	6477.0
21	7217.5
22	7958.0
23	8253.0
24	8548.0
25	10698.0
26	15339.0
27	17830.0
28	21684.5
29	25539.0
30	30798.0
31	36057.0
32	38826.5
33	41596.0
34	46379.0
35	51162.0
36	50651.0
37	50140.0
38	61232.5
39	72325.0
40	95414.0
41	118503.0
42	136482.0
43	154461.0
44	155707.0
45	156953.0
46	155068.5
47	153184.0
48	159816.5
49	166449.0
50	163025.5
51	159602.0
52	147413.0
53	135224.0
54	118206.0
55	101188.0
56	86177.0
57	71166.0
58	66232.0
59	61298.0
60	52078.5
61	42859.0
62	36618.0
63	30377.0
64	23914.0
65	17451.0
66	13180.0
67	8909.0
68	6497.0
69	4085.0
70	3492.0
71	2899.0
72	2493.0
73	2087.0
74	1539.0
75	762.5
76	534.0
77	402.5
78	271.0
79	334.5
80	398.0
81	266.5
82	135.0
83	104.5
84	74.0
85	43.5
86	13.0
87	9.5
88	6.0
89	5.5
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1788572.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.463269200717868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.74830707529598	13.577396163559715
2	8.865781535880911	3.096510592716845
3	3.154250168977853	1.652505594818102
4	1.5368641888051573	1.073546922161894
5	0.9285514823491936	0.8107772251494796
6	0.6067791596904806	0.6357808686636142
7	0.4292376173188569	0.5247124443619742
8	0.33995599742298604	0.4749394479516924
9	0.278887990485982	0.4383266449233553
>10	2.970003703015488	12.681509966994179
>50	1.4816859551541388	19.076908246458153
>100	1.6483672532559117	43.6564762146698
>500	0.008091337390808868	0.9073798055028578
>1k	0.0029128814606911926	0.8494463122525507
>5k	3.2365349563235474E-4	0.5437835498158089
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9621	0.537915163605379	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2937	0.16420921271271158	No Hit
GAGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	1918	0.10723638746441294	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1182105053640558E-4	0.0	0.0	0.10572680328217147	0.0
2	1.1182105053640558E-4	0.0	0.0	0.35603822490791537	0.0
3	1.1182105053640558E-4	0.0	0.0	0.5226515902071597	0.0
4	1.1182105053640558E-4	0.0	0.0	0.9527153505701755	0.0
5	1.1182105053640558E-4	0.0	0.0	1.517803029455901	0.0
6	1.1182105053640558E-4	0.0	0.0	2.2765647678706813	0.0
7	1.1182105053640558E-4	0.0	0.0	2.615326640470722	0.0
8	1.1182105053640558E-4	0.0	0.0	3.541987686265915	0.0
9	1.1182105053640558E-4	0.0	0.0	3.8244476599208754	0.0
10	1.1182105053640558E-4	0.0	0.0	4.3812046705416385	0.0
11	1.1182105053640558E-4	0.0	0.0	5.021324274337292	0.0
12	1.1182105053640558E-4	0.0	0.0	5.5002538337847175	0.0
13	1.1182105053640558E-4	0.0	0.0	5.7105333193184284	0.0
14	1.1182105053640558E-4	0.0	0.0	5.783943838995579	0.0
15	1.1182105053640558E-4	0.0	0.0	5.885589173933171	0.0
16	1.1182105053640558E-4	0.0	0.0	6.13137184301219	0.0
17	1.1182105053640558E-4	0.0	0.0	6.401531501108146	0.0
18	1.1182105053640558E-4	0.0	0.0	6.738783789525946	0.0
19	1.1182105053640558E-4	0.0	0.0	6.906235812704213	0.0
20	1.1182105053640558E-4	0.0	0.0	7.0756447042668675	0.0
21	1.1182105053640558E-4	0.0	0.0	7.323719704881883	0.0
22	1.1182105053640558E-4	0.0	0.0	7.588903326228969	0.0
23	1.1182105053640558E-4	0.0	0.0	7.857609310667952	0.0
24	1.1182105053640558E-4	0.0	0.0	8.062800938402257	0.0
25	2.2364210107281117E-4	0.0	0.0	8.232321651015447	0.0
26	2.2364210107281117E-4	0.0	0.0	8.392896679585725	0.0
27	2.2364210107281117E-4	0.0	0.0	8.554757650237173	0.0
28	2.2364210107281117E-4	0.0	0.0	8.719525968202566	0.0
29	2.2364210107281117E-4	0.0	0.0	8.90201792267798	0.0
30	2.2364210107281117E-4	0.0	0.0	9.134773439369509	0.0
31	2.2364210107281117E-4	0.0	0.0	9.342928324943028	0.0
32	2.2364210107281117E-4	0.0	0.0	9.530843600369456	0.0
33	2.2364210107281117E-4	0.0	0.0	9.73329561236562	0.0
34	2.2364210107281117E-4	0.0	0.0	9.924845071934481	0.0
35	2.2364210107281117E-4	0.0	0.0	10.142504746803596	0.0
36	2.2364210107281117E-4	0.0	0.0	10.350995095528724	0.0
37	2.2364210107281117E-4	0.0	0.0	10.562169149466726	0.0
38	2.2364210107281117E-4	0.0	0.0	10.757017330026413	0.0
39	2.2364210107281117E-4	0.0	0.0	10.95057956850493	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	35	1.2124292E-7	45.000004	1
TCGAGTA	35	1.2124292E-7	45.000004	1
TCCATCG	25	3.8916798E-5	45.0	18
GGTCGAA	20	7.03434E-4	45.0	17
CGCGGAT	20	7.03434E-4	45.0	23
ACGTTAG	25	3.8916798E-5	45.0	1
GCGAACG	45	3.8562575E-10	45.0	1
CGTTGCC	25	3.8916798E-5	45.0	16
TAACGCG	40	6.8193913E-9	45.0	1
CGCGAAC	20	7.03434E-4	45.0	40
CGTTAGT	20	7.03434E-4	45.0	31
TACGCGG	110	0.0	45.0	2
CGCTATA	25	3.8916798E-5	45.0	31
CTACGAT	20	7.03434E-4	45.0	39
CGCTAAC	45	3.8562575E-10	45.0	24
GTCGTAA	25	3.8916798E-5	45.0	10
GTCCGAT	25	3.8916798E-5	45.0	8
CGTACGT	20	7.03434E-4	45.0	12
TCCGTCT	20	7.03434E-4	45.0	24
TTACGGC	25	3.8916798E-5	45.0	14
>>END_MODULE