FastQCFastQC Report
Thu 26 May 2016
SRR1547426_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547426_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2239511
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116190.5188186170998937No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC34040.15199746730424632No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCGT253.8919956E-545.035
ATCGTAC302.166429E-644.99999640
CGTTTTT63800.042.0023541
TTGCGAA603.6379788E-1241.24999618
AACGGGT3150.040.7142874
GCGCGAC6700.040.634339
AGGGCGA18400.040.230986
ACGGGTC2300.040.10875
CGGTCTA3050.039.83606731
CGAGGGC8600.039.7674454
CGACGGT3000.039.7528
ACGCCTA403.4601726E-739.37529
TAAACGG1500.039.02
TAGGGCG9850.038.604065
CTTCGAA356.2509444E-638.5714321
ACGGGTA3150.038.571435
TCCTACG356.2509444E-638.5714339
CGAGGGT4200.038.571434
TAGGGTA12800.038.3203125
AATGGGC11450.037.9257624
Produced by FastQC (version 0.11.5)