##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547426_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2239511
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.336151061548705	31.0	31.0	33.0	30.0	34.0
2	31.76068614978895	31.0	31.0	34.0	30.0	34.0
3	31.928360700170707	31.0	31.0	34.0	30.0	34.0
4	35.67857760019933	37.0	35.0	37.0	33.0	37.0
5	35.56727026569639	37.0	35.0	37.0	33.0	37.0
6	35.61767412618201	37.0	35.0	37.0	33.0	37.0
7	35.92010621961669	37.0	35.0	37.0	35.0	37.0
8	35.95315004034363	37.0	35.0	37.0	35.0	37.0
9	37.77993945999819	39.0	38.0	39.0	35.0	39.0
10	37.059675973906806	39.0	37.0	39.0	33.0	39.0
11	36.827817769146925	39.0	37.0	39.0	32.0	39.0
12	36.312106080300566	38.0	35.0	39.0	32.0	39.0
13	36.124280255823706	38.0	35.0	39.0	32.0	39.0
14	37.19492246298411	39.0	35.0	41.0	32.0	41.0
15	37.367367251154384	39.0	35.0	41.0	32.0	41.0
16	37.44158345281626	39.0	35.0	41.0	32.0	41.0
17	37.369132815154735	39.0	35.0	41.0	32.0	41.0
18	37.30147563463631	39.0	35.0	41.0	32.0	41.0
19	37.2256586370864	39.0	35.0	41.0	32.0	41.0
20	37.097814210334306	39.0	35.0	41.0	32.0	41.0
21	37.02730819361906	39.0	35.0	41.0	32.0	41.0
22	36.95170910078138	39.0	35.0	41.0	31.0	41.0
23	36.885240572607145	38.0	35.0	41.0	31.0	41.0
24	36.80116328966457	38.0	35.0	41.0	31.0	41.0
25	36.69681015185905	38.0	35.0	40.0	31.0	41.0
26	36.58258476962158	38.0	35.0	40.0	31.0	41.0
27	36.51169384745152	38.0	35.0	40.0	31.0	41.0
28	36.447014102632224	38.0	35.0	40.0	31.0	41.0
29	36.470000370616624	38.0	35.0	40.0	31.0	41.0
30	36.43127673853801	38.0	35.0	40.0	31.0	41.0
31	36.307200991645054	38.0	35.0	40.0	30.0	41.0
32	36.230639635170355	38.0	35.0	40.0	30.0	41.0
33	36.15584027048762	38.0	35.0	41.0	30.0	41.0
34	36.06719323995283	38.0	35.0	41.0	30.0	41.0
35	35.96488876366314	38.0	35.0	41.0	30.0	41.0
36	35.88291327883632	38.0	35.0	41.0	29.0	41.0
37	35.830788506955315	38.0	35.0	41.0	29.0	41.0
38	35.7331774659736	38.0	35.0	40.0	29.0	41.0
39	35.67785646062913	38.0	35.0	40.0	29.0	41.0
40	35.568653156872195	38.0	34.0	40.0	28.0	41.0
41	35.476844721905806	38.0	34.0	40.0	28.0	41.0
42	35.427171378037436	38.0	34.0	40.0	27.0	41.0
43	35.3768599484441	38.0	34.0	40.0	27.0	41.0
44	35.25874934304855	38.0	34.0	40.0	27.0	41.0
45	35.11841111742697	37.0	34.0	40.0	27.0	41.0
46	35.10912828738059	37.0	34.0	40.0	27.0	41.0
47	35.07068462713512	37.0	34.0	40.0	27.0	41.0
48	35.00483141185732	37.0	34.0	40.0	27.0	41.0
49	34.93926218714711	37.0	34.0	40.0	26.0	41.0
50	34.824572417818	37.0	34.0	40.0	26.0	41.0
51	34.25596837881127	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	0.0
12	5.0
13	11.0
14	20.0
15	48.0
16	141.0
17	331.0
18	730.0
19	1551.0
20	2640.0
21	4322.0
22	6900.0
23	10108.0
24	15041.0
25	21523.0
26	28342.0
27	33545.0
28	38120.0
29	44722.0
30	54307.0
31	66395.0
32	83134.0
33	108039.0
34	176533.0
35	255375.0
36	171643.0
37	215914.0
38	325168.0
39	574612.0
40	287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.9270541649494	24.56143327717524	29.113051911778953	13.398460646096403
2	30.07875379937853	25.194071384333455	30.985692858842846	13.741481957445176
3	28.253087392738863	25.623182918056663	32.073921494469104	14.04980819473537
4	24.993938408875866	28.133507716639926	31.54585978814125	15.326694086342957
5	23.255969718389416	31.95688701685323	30.557161808984194	14.229981455773158
6	20.404945543915616	41.71232023419398	26.917126104761262	10.965608117129142
7	89.94048254284083	3.4424479272484034	5.325448278664405	1.2916212512463658
8	91.44165846919262	2.449775866249373	4.597074986459098	1.5114906780989243
9	87.84841869497404	3.8654420540912726	6.27203885133853	2.014100399596162
10	40.79524503340238	27.587451010510776	15.856229328634688	15.761074627452153
11	35.000453223940404	26.08681091541859	23.255791107969554	15.656944752671453
12	32.872711944705785	23.3479094320144	25.701101713722323	18.078276909557488
13	22.915270342498875	31.19408656621914	27.12051872038137	18.77012437090061
14	18.905466416552542	33.44261314188678	28.748240129206774	18.903680312353902
15	18.169725444527845	26.418803033340758	37.30100008439342	18.11047143773797
16	21.15497534952943	23.506962010903273	36.3139542516201	19.024108387947187
17	21.124477620337654	23.97036674524037	28.525736198661228	26.379419435760752
18	23.665166190297793	25.39438297021091	31.111211331402256	19.829239508089042
19	26.531952734324594	27.368921161807197	25.44309896222881	20.656027141639402
20	28.035852469579297	27.030365110955024	26.06372551865117	18.870056900814507
21	24.153487078205913	29.076392123101876	27.93882235898819	18.831298439704025
22	24.27503146892335	24.44797100795665	27.671665823476644	23.605331699643358
23	21.67794665889116	29.31622126437423	26.95217839965957	22.05365367707504
24	21.453924539776764	26.1349910761769	33.85274731849944	18.558337065546898
25	20.765827897250784	27.10350607788932	30.58319427767937	21.54747174718052
26	19.574005218103416	31.752467391318905	26.734854171290074	21.9386732192876
27	19.439199003711078	30.970957499204065	29.04509064702071	20.544752850064143
28	17.627151641586043	29.3183645894126	33.46936005226141	19.58512371673995
29	19.047059826899712	26.801118637059606	32.82216519588428	21.3296563401564
30	20.721889733964243	29.4000342038954	30.371898150980282	19.50617791116007
31	25.499316591880994	27.9448504606586	27.1384690675777	19.417363879882707
32	26.067029811418653	27.493814497897084	27.220317292480367	19.218838398203893
33	24.95276870709722	29.08349188729147	25.89333117810093	20.07040822751038
34	20.683399188483556	27.855857818961372	29.221066563191695	22.239676429363374
35	20.522962378840738	27.522347512470358	30.07357409720247	21.881116011486437
36	25.52950175283801	28.035629206554468	26.78508835187682	19.649780688730708
37	21.154573476084735	30.794267141353625	28.324397602869556	19.726761779692083
38	21.404181537844646	30.647672639250267	26.232557018027595	21.71558880487749
39	21.090675598378397	29.826377276110723	27.033267530277815	22.049679595233066
40	23.780995047579584	27.207769910484924	27.115294365600345	21.895940676335147
41	19.783158019764137	26.056179228411917	28.5407394739298	25.619923277894145
42	22.47910369719104	27.52743790943648	25.7558904600156	24.23756793335688
43	21.837669026854524	27.243402689247787	26.994017890512705	23.924910393384984
44	21.399939540372877	28.577265304791982	27.962711502645	22.060083652190144
45	19.762707126689712	31.519068225161657	26.35704848067279	22.361176167475847
46	21.81784327024962	29.984626108110206	26.974727965167393	21.222802656472776
47	21.36310114127593	27.92368512590472	28.034378933615418	22.678834799203933
48	21.869417028985346	27.23121252809207	29.73095465929839	21.16841578362419
49	21.916793442854264	26.8835919984318	29.28996553265423	21.909649026059707
50	20.598693196863067	29.889382101717743	28.39776183282868	21.11416286859051
51	19.120736625093603	30.99225679177285	26.981649118937124	22.905357464196424
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2492.0
1	2782.0
2	3072.0
3	10578.0
4	18084.0
5	12604.0
6	7124.0
7	7580.0
8	8036.0
9	8965.0
10	9894.0
11	10327.0
12	10760.0
13	10673.5
14	10587.0
15	10270.5
16	9954.0
17	9389.5
18	8825.0
19	8643.0
20	8461.0
21	8985.0
22	9509.0
23	9179.5
24	8850.0
25	11123.0
26	17064.5
27	20733.0
28	25165.5
29	29598.0
30	34106.0
31	38614.0
32	44756.5
33	50899.0
34	57619.0
35	64339.0
36	70925.0
37	77511.0
38	88498.5
39	99486.0
40	115537.0
41	131588.0
42	155075.0
43	178562.0
44	189217.5
45	199873.0
46	197806.5
47	195740.0
48	192084.5
49	188429.0
50	172760.0
51	157091.0
52	152687.0
53	148283.0
54	137557.5
55	126832.0
56	118940.0
57	111048.0
58	99163.5
59	87279.0
60	77058.5
61	66838.0
62	58151.5
63	49465.0
64	39957.5
65	30450.0
66	24890.0
67	19330.0
68	16033.0
69	12736.0
70	11025.5
71	9315.0
72	8273.5
73	7232.0
74	5719.0
75	3438.5
76	2671.0
77	1995.5
78	1320.0
79	906.5
80	493.0
81	389.5
82	286.0
83	219.0
84	152.0
85	97.0
86	42.0
87	25.5
88	9.0
89	8.5
90	8.0
91	7.0
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2239511.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.458815840389036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.31039065899954	12.88896298245395
2	7.150850336781671	2.3538905759054685
3	2.563144308910844	1.2655896045811441
4	1.3312619086324653	0.8764397835802623
5	0.884055741730877	0.7275255322893518
6	0.6486407632421662	0.640551532126332
7	0.48949276281702897	0.5639529866886096
8	0.4152264415035544	0.5467308426213657
9	0.34214707968129765	0.5068202197321252
>10	4.289219164480754	17.49938877408504
>50	2.0858027525375125	24.903064861722342
>100	1.4829078482263773	35.80643229471407
>500	0.0054881859647648605	0.5658662841845362
>1k	0.001097637192952972	0.3300171552826568
>5k	0.0	0.0
>10k+	2.74409298238243E-4	0.5247665700327342
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11619	0.5188186170998937	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3404	0.15199746730424632	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.930520993198961E-5	0.0	0.0	0.029381414067624585	0.0
2	8.930520993198961E-5	0.0	0.0	0.07885650036994683	0.0
3	8.930520993198961E-5	0.0	0.0	0.12373236836077162	0.0
4	8.930520993198961E-5	0.0	0.0	0.22219136231079017	0.0
5	8.930520993198961E-5	0.0	0.0	0.3632935940033338	0.0
6	8.930520993198961E-5	0.0	0.0	0.696446679654621	0.0
7	8.930520993198961E-5	0.0	0.0	0.8444700651168938	0.0
8	8.930520993198961E-5	0.0	0.0	1.2673748867498307	0.0
9	8.930520993198961E-5	0.0	0.0	1.4609439292774182	0.0
10	8.930520993198961E-5	0.0	0.0	1.7669482311093805	0.0
11	8.930520993198961E-5	0.0	0.0	2.074024195460527	0.0
12	8.930520993198961E-5	0.0	0.0	2.3079591928773735	0.0
13	8.930520993198961E-5	0.0	0.0	2.4356656430801187	0.0
14	8.930520993198961E-5	0.0	0.0	2.4896952950889726	0.0
15	8.930520993198961E-5	0.0	0.0	2.544975220036874	0.0
16	8.930520993198961E-5	0.0	0.0	2.656517427241929	0.0
17	8.930520993198961E-5	0.0	0.0	2.779535353923245	0.0
18	8.930520993198961E-5	0.0	0.0	2.9371143968482407	0.0
19	8.930520993198961E-5	0.0	0.0	3.0235618400624067	0.0
20	8.930520993198961E-5	0.0	0.0	3.1209491714932414	0.0
21	8.930520993198961E-5	0.0	0.0	3.244770845063945	0.0
22	8.930520993198961E-5	0.0	0.0	3.3785500495420653	0.0
23	8.930520993198961E-5	0.0	0.0	3.5259036459298483	0.0
24	8.930520993198961E-5	0.0	0.0	3.6402589672477608	0.0
25	8.930520993198961E-5	0.0	0.0	3.7392091398524054	0.0
26	8.930520993198961E-5	0.0	0.0	3.835122935319362	0.0
27	8.930520993198961E-5	0.0	0.0	3.9318851302806728	0.0
28	8.930520993198961E-5	0.0	0.0	4.036729446740829	0.0
29	8.930520993198961E-5	0.0	0.0	4.152424346207721	0.0
30	8.930520993198961E-5	0.0	0.0	4.305493476031152	0.0
31	8.930520993198961E-5	0.0	0.0	4.434092978333217	0.0
32	8.930520993198961E-5	0.0	0.0	4.5642553218090915	0.0
33	8.930520993198961E-5	0.0	0.0	4.695578633014082	0.0
34	8.930520993198961E-5	0.0	0.0	4.83261747765472	0.0
35	8.930520993198961E-5	0.0	0.0	4.994304560236587	0.0
36	8.930520993198961E-5	0.0	0.0	5.137639422177431	0.0
37	8.930520993198961E-5	0.0	0.0	5.287002385788683	0.0
38	8.930520993198961E-5	0.0	0.0	5.461058239946131	0.0
39	8.930520993198961E-5	0.0	0.0	5.740137020983599	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACCGT	25	3.8919956E-5	45.0	35
ATCGTAC	30	2.166429E-6	44.999996	40
CGTTTTT	6380	0.0	42.002354	1
TTGCGAA	60	3.6379788E-12	41.249996	18
AACGGGT	315	0.0	40.714287	4
GCGCGAC	670	0.0	40.63433	9
AGGGCGA	1840	0.0	40.23098	6
ACGGGTC	230	0.0	40.1087	5
CGGTCTA	305	0.0	39.836067	31
CGAGGGC	860	0.0	39.767445	4
CGACGGT	300	0.0	39.75	28
ACGCCTA	40	3.4601726E-7	39.375	29
TAAACGG	150	0.0	39.0	2
TAGGGCG	985	0.0	38.60406	5
CTTCGAA	35	6.2509444E-6	38.57143	21
ACGGGTA	315	0.0	38.57143	5
TCCTACG	35	6.2509444E-6	38.57143	39
CGAGGGT	420	0.0	38.57143	4
TAGGGTA	1280	0.0	38.320312	5
AATGGGC	1145	0.0	37.925762	4
>>END_MODULE