FastQCFastQC Report
Thu 26 May 2016
SRR1547425_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547425_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1391745
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118420.8508742621672792No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24180.17373872368860677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC502.1827873E-1145.025
CTATCGA253.8912323E-545.043
TAGCGCG207.0338E-445.01
TAACGCG406.8175723E-945.01
TCGATCA207.0338E-445.017
CATTACG253.8912323E-545.035
TCGGACG253.8912323E-545.011
CCTCACG406.8175723E-945.045
AACGCCA351.2121745E-745.030
GTCGTTG207.0338E-445.01
CGTAAAC502.1827873E-1145.035
TATCGTC207.0338E-445.022
CCGTTCC351.2121745E-745.016
TCGAACT207.0338E-445.016
CGGACTA253.8912323E-545.011
CTTAACG302.1658343E-644.9999961
ACTAACG302.1658343E-644.99999625
TACTCGC1350.043.33333245
CGTTAGG1250.043.22
GCGCGAC750.042.09