##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547425_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1391745
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.296008248637502	31.0	31.0	33.0	30.0	34.0
2	31.719327175596103	31.0	31.0	34.0	30.0	34.0
3	31.83991463953526	31.0	31.0	34.0	30.0	34.0
4	35.60438657943804	37.0	35.0	37.0	33.0	37.0
5	35.479818501234064	37.0	35.0	37.0	33.0	37.0
6	35.51240708606821	37.0	35.0	37.0	33.0	37.0
7	35.91021702970013	37.0	35.0	37.0	35.0	37.0
8	35.89004091985242	37.0	35.0	37.0	35.0	37.0
9	37.612411756464006	39.0	37.0	39.0	35.0	39.0
10	36.953502257956735	39.0	37.0	39.0	32.0	39.0
11	36.61680480260392	39.0	35.0	39.0	32.0	39.0
12	36.024819201793434	38.0	35.0	39.0	31.0	39.0
13	35.72001192747235	38.0	35.0	39.0	30.0	39.0
14	36.81028708563709	39.0	35.0	41.0	31.0	41.0
15	37.03976446834729	39.0	35.0	41.0	32.0	41.0
16	37.1501956177317	39.0	35.0	41.0	32.0	41.0
17	37.050272858892974	39.0	35.0	41.0	32.0	41.0
18	36.96499933536675	38.0	35.0	41.0	32.0	41.0
19	36.905971999180885	38.0	35.0	41.0	31.0	41.0
20	36.74393513179498	38.0	35.0	40.0	31.0	41.0
21	36.621115218664336	38.0	35.0	40.0	31.0	41.0
22	36.53086017912764	38.0	35.0	40.0	31.0	41.0
23	36.47629486723502	38.0	35.0	40.0	31.0	41.0
24	36.32555245393373	38.0	35.0	40.0	31.0	41.0
25	36.25546993163259	38.0	35.0	40.0	30.0	41.0
26	36.100638407179474	38.0	35.0	40.0	30.0	41.0
27	35.999236929178835	38.0	35.0	40.0	30.0	41.0
28	36.101956177316964	38.0	35.0	40.0	30.0	41.0
29	36.15589781173994	38.0	35.0	40.0	30.0	41.0
30	36.0980431041606	38.0	35.0	40.0	30.0	41.0
31	35.95748430926643	38.0	35.0	40.0	30.0	41.0
32	35.839832009455755	38.0	34.0	40.0	30.0	41.0
33	35.61686012883107	38.0	34.0	40.0	29.0	41.0
34	35.4271055401672	38.0	34.0	40.0	27.0	41.0
35	35.282715583673735	38.0	34.0	40.0	27.0	41.0
36	35.10857161333434	37.0	34.0	40.0	26.0	41.0
37	35.0298783182264	37.0	34.0	40.0	26.0	41.0
38	34.98662757904645	37.0	34.0	40.0	26.0	41.0
39	34.96916892103079	37.0	34.0	40.0	26.0	41.0
40	34.79198703785536	37.0	34.0	40.0	25.0	41.0
41	34.7211378521209	37.0	34.0	40.0	24.0	41.0
42	34.639337666023586	37.0	33.0	40.0	24.0	41.0
43	34.55962981724382	37.0	33.0	40.0	24.0	41.0
44	34.40417748941078	36.0	33.0	40.0	24.0	41.0
45	34.32481022026305	36.0	33.0	40.0	23.0	41.0
46	34.21945758741724	36.0	33.0	40.0	23.0	41.0
47	34.10637940139896	36.0	33.0	40.0	23.0	41.0
48	34.05770094377921	36.0	33.0	40.0	23.0	41.0
49	33.94369801939292	36.0	33.0	40.0	23.0	41.0
50	33.860988183898634	35.0	33.0	40.0	23.0	41.0
51	33.411092549281655	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	1.0
13	3.0
14	18.0
15	55.0
16	124.0
17	311.0
18	689.0
19	1369.0
20	2390.0
21	3654.0
22	5567.0
23	7942.0
24	11423.0
25	15917.0
26	21701.0
27	26524.0
28	29544.0
29	33566.0
30	39641.0
31	47256.0
32	57691.0
33	74105.0
34	118740.0
35	166580.0
36	107511.0
37	131358.0
38	188374.0
39	299609.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.039953439746505	23.921120607582566	30.055721414483255	14.983204538187673
2	29.52250591882852	26.335212269489023	30.486260054823262	13.656021756859193
3	27.076080747550733	25.913008489342516	33.087742366597325	13.92316839650942
4	24.72715907008827	28.473966136037852	31.674624302584164	15.124250491289711
5	22.719391842614847	32.30505588308203	30.834815285846183	14.140736988456936
6	22.0291073436585	41.83711815023585	26.201495245177814	9.932279260927828
7	89.18192628678385	3.0604025881177948	6.415040111514682	1.3426310135836665
8	89.24034216038139	2.4439103427711255	6.329176681073041	1.9865708157744415
9	84.5954539085824	4.997395356189531	7.913662344754248	2.4934883904738294
10	44.20299695705751	33.36243349176753	12.47901016349978	9.955559387675185
11	35.67420755957449	22.995735569375135	25.48656542685621	15.84349144419416
12	35.302947019748586	20.70566087896849	26.994456599448892	16.996935501834027
13	22.653790744712573	31.317446802395555	28.175348213932867	17.853414238959004
14	17.36798048493079	32.96465947425713	30.068223704773505	19.59913633603857
15	16.97257759144096	25.94246790899195	39.64871438374127	17.436240115825814
16	20.032908327315706	23.96279490855006	37.99137054561001	18.01292621852423
17	19.41275161757362	24.496082256447842	28.82381470743563	27.26735141854291
18	21.529303141020804	25.844533301718347	32.218689486939056	20.40747407032179
19	25.305425922133722	26.690090497900123	27.109204631595585	20.89527894837057
20	27.295301941088347	26.07381380928259	27.99471167491171	18.636172574717353
21	23.608275941354197	28.081293627783825	29.50626731189981	18.804163118962165
22	24.529277992735736	24.056993199185193	28.143086556804587	23.270642251274477
23	21.370294127156914	27.72857096666415	27.881688096598158	23.019446809580778
24	20.843257924404256	25.37232036041085	34.71670456872488	19.06771714646002
25	19.78875440544065	27.354364484873305	31.644230803775113	21.212650305910923
26	19.499118013716593	32.3433531286263	26.999989222163546	21.15753963549357
27	19.350240166122386	31.537170961634498	28.75483655411013	20.35775231813299
28	17.470298079030282	29.311332176512224	33.960172301678824	19.25819744277867
29	18.066240582865394	24.91886085453873	34.04704166352313	22.967856899072746
30	20.488810809451444	28.956453948101124	30.718845765567686	19.835889476879746
31	25.780584805406164	26.28132308720347	27.71614052861695	20.22195157877341
32	26.60940042895789	26.264222253358195	27.535791398567984	19.59058591911593
33	25.146345056026785	26.540565980118487	27.61396663900355	20.699122324851178
34	21.1346906222045	26.39822668664159	29.62381758152535	22.84326510962856
35	20.72542024580652	26.574049125378572	30.36411124164269	22.336419387172217
36	26.090196120697396	26.850967670083243	27.298104178567193	19.760732030652168
37	21.406435805409753	29.524733338362992	30.262152908758427	18.806677947468824
38	20.53206585976598	30.09509644367323	27.40365512360382	21.96918257295697
39	21.04875533951981	29.5809936446691	28.076695084228792	21.293555931582294
40	23.90452273943862	26.279095667669004	27.504320116113224	22.312061476779153
41	18.312837481004063	25.627252118743016	29.589687766077837	26.470222634175084
42	21.30634563084473	25.578608150199926	27.36334601525423	25.751700203701112
43	21.57363597498105	26.15004903915588	28.04630158541974	24.23001340044333
44	20.10885614821681	28.21263952807447	28.809156849853963	22.869347473854766
45	19.826189424068346	31.23345153027315	25.61223499994611	23.328124045712396
46	21.290538137374305	30.731922873802315	27.000995153566205	20.97654383525718
47	20.83485121196771	26.06864044778318	29.09290135764813	24.00360698260098
48	21.634710381571338	25.034111852386754	32.216749476376776	21.11442828966513
49	21.142522516696665	24.866767978329364	31.251342738791948	22.739366766182023
50	19.065920840383836	28.849034844745265	30.022884939410595	22.062159375460304
51	19.0488918587816	28.16097776532339	28.408149481406436	24.38198089448857
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1388.0
1	1911.5
2	2435.0
3	9986.5
4	17538.0
5	11690.0
6	5842.0
7	6118.0
8	6394.0
9	7163.5
10	7933.0
11	8479.5
12	9026.0
13	8830.5
14	8635.0
15	8218.0
16	7801.0
17	7147.5
18	6494.0
19	6465.5
20	6437.0
21	6474.0
22	6511.0
23	6833.0
24	7155.0
25	8357.5
26	11742.5
27	13925.0
28	17329.5
29	20734.0
30	23283.5
31	25833.0
32	29880.5
33	33928.0
34	38181.0
35	42434.0
36	44542.5
37	46651.0
38	51485.0
39	56319.0
40	68685.0
41	81051.0
42	91343.5
43	101636.0
44	106325.0
45	111014.0
46	111699.5
47	112385.0
48	109321.5
49	106258.0
50	103059.0
51	99860.0
52	90804.0
53	81748.0
54	77270.5
55	72793.0
56	71775.5
57	70758.0
58	63405.5
59	56053.0
60	52640.0
61	49227.0
62	42882.5
63	36538.0
64	31307.5
65	26077.0
66	20983.0
67	15889.0
68	13254.0
69	10619.0
70	8813.0
71	7007.0
72	5384.0
73	3761.0
74	3268.0
75	2258.5
76	1742.0
77	1307.5
78	873.0
79	636.5
80	400.0
81	282.5
82	165.0
83	104.5
84	44.0
85	55.0
86	66.0
87	39.5
88	13.0
89	10.5
90	8.0
91	6.0
92	4.0
93	4.5
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1391745.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.01182002426246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.07820937396436	17.246125632794712
2	7.061796836812244	2.967624083660096
3	2.1241274425004875	1.3389535059125146
4	1.029504326085009	0.8652703845559129
5	0.6116566488219619	0.6426009710845284
6	0.39423020290808064	0.4970096442979836
7	0.3058957528149332	0.44991985530335565
8	0.22828165061269373	0.38372903660123875
9	0.2000140452605776	0.3782393209205948
>10	3.0925478247017786	17.27691631803069
>50	1.849907308757974	28.24966588271532
>100	1.0214211967339473	28.404017925458337
>500	0.0017195643042658994	0.2694663082317408
>1k	3.439128608531799E-4	0.17473036559515934
>5k	0.0	0.0
>10k+	3.439128608531799E-4	0.8557307648378316
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11842	0.8508742621672792	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2418	0.17373872368860677	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.185224304739733E-5	0.0	0.0	0.039446881433021136	0.0
2	7.185224304739733E-5	0.0	0.0	0.21447894549648103	0.0
3	7.185224304739733E-5	0.0	0.0	0.3131320752005576	0.0
4	7.185224304739733E-5	0.0	0.0	0.6023373534663319	0.0
5	7.185224304739733E-5	0.0	0.0	1.0646346852332862	0.0
6	7.185224304739733E-5	0.0	0.0	1.5919582969581354	0.0
7	7.185224304739733E-5	0.0	0.0	1.8397048309855613	0.0
8	7.185224304739733E-5	0.0	0.0	2.316013350146758	0.0
9	7.185224304739733E-5	0.0	0.0	2.472291978774847	0.0
10	7.185224304739733E-5	0.0	0.0	2.730816349259383	0.0
11	7.185224304739733E-5	0.0	0.0	3.1612831373563406	0.0
12	7.185224304739733E-5	0.0	0.0	3.482175254806017	0.0
13	7.185224304739733E-5	0.0	0.0	3.6021685006951705	0.0
14	7.185224304739733E-5	0.0	0.0	3.64635763016932	0.0
15	7.185224304739733E-5	0.0	0.0	3.720149883778997	0.0
16	7.185224304739733E-5	0.0	0.0	3.8902241430721864	0.0
17	7.185224304739733E-5	0.0	0.0	4.0699984551767745	0.0
18	7.185224304739733E-5	0.0	0.0	4.289507057686573	0.0
19	7.185224304739733E-5	0.0	0.0	4.415823300963898	0.0
20	7.185224304739733E-5	0.0	0.0	4.544582520504834	0.0
21	7.185224304739733E-5	0.0	0.0	4.717890130735157	0.0
22	7.185224304739733E-5	0.0	0.0	4.901688168450399	0.0
23	7.185224304739733E-5	0.0	0.0	5.0911625333663855	0.0
24	7.185224304739733E-5	0.0	0.0	5.243632993112962	0.0
25	7.185224304739733E-5	0.0	0.0	5.371098872279045	0.0
26	7.185224304739733E-5	0.0	0.0	5.5006484664935025	0.0
27	7.185224304739733E-5	0.0	0.0	5.620929121354846	0.0
28	7.185224304739733E-5	0.0	0.0	5.743221639021516	0.0
29	2.1555672914219199E-4	0.0	0.0	5.878806821651955	0.0
30	2.1555672914219199E-4	0.0	0.0	6.036953608599276	0.0
31	2.1555672914219199E-4	0.0	0.0	6.184969229276915	0.0
32	2.1555672914219199E-4	0.0	0.0	6.320482559664306	0.0
33	2.1555672914219199E-4	0.0	0.0	6.4612411037941575	0.0
34	2.1555672914219199E-4	0.0	0.0	6.6114122917632185	0.0
35	2.1555672914219199E-4	0.0	0.0	6.7852228676948725	0.0
36	2.1555672914219199E-4	0.0	0.0	6.93604072585136	0.0
37	2.1555672914219199E-4	0.0	0.0	7.0921756499933535	0.0
38	2.1555672914219199E-4	0.0	0.0	7.256214320870562	0.0
39	2.1555672914219199E-4	0.0	0.0	7.426288580163751	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	50	2.1827873E-11	45.0	25
CTATCGA	25	3.8912323E-5	45.0	43
TAGCGCG	20	7.0338E-4	45.0	1
TAACGCG	40	6.8175723E-9	45.0	1
TCGATCA	20	7.0338E-4	45.0	17
CATTACG	25	3.8912323E-5	45.0	35
TCGGACG	25	3.8912323E-5	45.0	11
CCTCACG	40	6.8175723E-9	45.0	45
AACGCCA	35	1.2121745E-7	45.0	30
GTCGTTG	20	7.0338E-4	45.0	1
CGTAAAC	50	2.1827873E-11	45.0	35
TATCGTC	20	7.0338E-4	45.0	22
CCGTTCC	35	1.2121745E-7	45.0	16
TCGAACT	20	7.0338E-4	45.0	16
CGGACTA	25	3.8912323E-5	45.0	11
CTTAACG	30	2.1658343E-6	44.999996	1
ACTAACG	30	2.1658343E-6	44.999996	25
TACTCGC	135	0.0	43.333332	45
CGTTAGG	125	0.0	43.2	2
GCGCGAC	75	0.0	42.0	9
>>END_MODULE