FastQCFastQC Report
Thu 26 May 2016
SRR1547424_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547424_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2578430
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200980.7794665746209903No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGC302.1665564E-645.00000418
ACGCGAT551.8189894E-1245.01
CTATCGT207.034921E-445.026
CGCGCGA207.034921E-445.013
CGCGAAA207.034921E-445.012
TACGCAA351.2127202E-745.018
TACGAGT253.89216E-545.022
CTACGAT207.034921E-445.020
GTCCGTT253.89216E-545.028
ACGCTCG207.034921E-445.028
AATTACG351.2127202E-745.01
CCGCGAA207.034921E-445.011
ATACCGG750.045.02
TACGTCG253.89216E-545.01
ATCGACG253.89216E-545.01
ATATCCG900.042.51
GACCGAT14400.042.343759
AGTACGG2500.042.3000032
CGTTTTT92900.042.2874071
ATCCGGC556.184564E-1140.90909210