FastQCFastQC Report
Thu 26 May 2016
SRR1547423_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547423_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2363910
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316411.3385027348756933No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26490.1120601038110588No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGT207.0348004E-445.0000041
CTCGCGA253.8920618E-545.026
CGATCGA253.8920618E-545.010
TCGCGAC253.8920618E-545.027
CGTTTTT130300.043.083271
GACGGGT1800.041.2500044
GCGATGT3500.040.59
TACGGGA9450.040.476194
TGCGTAG1450.040.3448261
AAGGGCG7200.040.0000045
CTCGTCC9200.039.37500437
TCGCGAG403.4602817E-739.3750041
CGTATGG3350.038.9552232
TAAGGGA36750.038.6938744
ACGGGAT13550.038.6900375
GGGCGAT33950.038.571437
TCGATAG1350.038.3333321
TCGTCCC9350.038.2620338
TATGGGA28800.038.203134
GGTCGGA659.094947E-1238.076928
Produced by FastQC (version 0.11.5)