##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547423_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2363910
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.301731030369176	31.0	31.0	33.0	30.0	34.0
2	31.724036871116073	31.0	31.0	34.0	30.0	34.0
3	31.80461861915217	31.0	31.0	34.0	30.0	34.0
4	35.58906811173014	37.0	35.0	37.0	33.0	37.0
5	35.4740366596021	37.0	35.0	37.0	33.0	37.0
6	35.51059811921774	37.0	35.0	37.0	33.0	37.0
7	35.94356468731889	37.0	35.0	37.0	35.0	37.0
8	35.934621876467375	37.0	35.0	37.0	35.0	37.0
9	37.6628230347179	39.0	37.0	39.0	35.0	39.0
10	36.92103168056313	39.0	37.0	39.0	32.0	39.0
11	36.63305540397054	39.0	35.0	39.0	32.0	39.0
12	36.34342635717942	38.0	35.0	39.0	32.0	39.0
13	36.196304427833546	39.0	35.0	39.0	32.0	39.0
14	37.32890423070252	39.0	36.0	41.0	32.0	41.0
15	37.48042142044325	39.0	36.0	41.0	32.0	41.0
16	37.54115723525853	39.0	35.0	41.0	32.0	41.0
17	37.450233299914125	39.0	35.0	41.0	32.0	41.0
18	37.326473089077	39.0	36.0	41.0	32.0	41.0
19	37.262199491520406	39.0	36.0	41.0	32.0	41.0
20	37.08436827121168	39.0	35.0	41.0	32.0	41.0
21	36.975996548091935	39.0	35.0	41.0	31.0	41.0
22	36.89764796460102	39.0	35.0	41.0	31.0	41.0
23	36.794009501207746	38.0	35.0	41.0	31.0	41.0
24	36.6556645557572	38.0	35.0	40.0	31.0	41.0
25	36.58393847481503	38.0	35.0	40.0	31.0	41.0
26	36.45697551937256	38.0	35.0	40.0	31.0	41.0
27	36.35626187122183	38.0	35.0	40.0	30.0	41.0
28	36.427723559695586	38.0	35.0	40.0	30.0	41.0
29	36.465575254557066	38.0	35.0	40.0	30.0	41.0
30	36.396418222351954	38.0	35.0	40.0	30.0	41.0
31	36.29674056964944	38.0	35.0	40.0	30.0	41.0
32	36.1670347009827	38.0	35.0	40.0	30.0	41.0
33	35.94767821109941	38.0	35.0	40.0	29.0	41.0
34	35.72363880181564	38.0	35.0	40.0	28.0	41.0
35	35.56536712480594	38.0	35.0	40.0	27.0	41.0
36	35.398297735531386	38.0	34.0	40.0	26.0	41.0
37	35.291737418091216	38.0	34.0	40.0	26.0	41.0
38	35.22940848001827	38.0	34.0	40.0	25.0	41.0
39	35.20145521614613	38.0	34.0	40.0	25.0	41.0
40	35.0582682927861	38.0	34.0	40.0	24.0	41.0
41	34.99931046444239	38.0	34.0	40.0	24.0	41.0
42	34.92941990177291	38.0	34.0	40.0	24.0	41.0
43	34.873719388640005	38.0	34.0	40.0	24.0	41.0
44	34.7318019721563	38.0	34.0	40.0	24.0	41.0
45	34.643718246464545	38.0	34.0	40.0	23.0	41.0
46	34.570147763662746	37.0	33.0	40.0	23.0	41.0
47	34.435291106683415	37.0	33.0	40.0	23.0	41.0
48	34.40154828229501	37.0	33.0	40.0	23.0	41.0
49	34.297402608390335	37.0	33.0	40.0	23.0	41.0
50	34.23016020068446	37.0	33.0	40.0	23.0	41.0
51	33.81575567597751	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	4.0
13	8.0
14	20.0
15	69.0
16	178.0
17	477.0
18	1025.0
19	2027.0
20	3589.0
21	5784.0
22	8769.0
23	12784.0
24	18917.0
25	27415.0
26	37853.0
27	44839.0
28	49005.0
29	53590.0
30	61522.0
31	73481.0
32	89383.0
33	113097.0
34	169476.0
35	238113.0
36	189561.0
37	231025.0
38	341624.0
39	590065.0
40	206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.67371854258411	24.435490352847612	30.035661256139196	15.855129848429087
2	31.30076864178416	26.05327613995457	29.524051254066357	13.121903964194914
3	26.028402096526516	24.803143943720364	35.220968649398664	13.947485310354454
4	23.323561387700884	27.95715572927903	34.38108895854749	14.33819392447259
5	22.2038910110791	32.75221983916477	32.039925377869714	13.00396377188641
6	21.238160505264585	40.50365707662305	28.49258220490628	9.765600213206087
7	88.70126189237322	2.5627456206031534	7.760447732781704	0.9755447542419128
8	89.43496156791079	2.087008388644238	7.290125258575834	1.1879047848691362
9	85.07840823043179	4.847477272823415	8.383398691151523	1.6907158055932756
10	36.08817594578474	37.783883481181604	15.350880532676795	10.777060040356865
11	27.00238164735544	26.057464116654188	31.14116019645418	15.798994039536193
12	29.58589794027691	22.520527431247384	30.09243160695627	17.80114302151943
13	20.969664665744467	29.160501034303337	31.148901607929236	18.720932692022963
14	16.57694243858692	31.169926097017232	31.797911087985582	20.455220376410267
15	16.24634609608657	27.45950564953826	38.80511525396483	17.489033000410338
16	19.088120952151307	25.53269794535325	37.572242598068456	17.806938504426988
17	18.831469895215978	26.606512092253936	30.656327863581943	23.905690148948143
18	19.024878273707543	27.847253068010204	33.72886446607527	19.399004192206977
19	22.72561138114395	28.455355745354094	29.47942180539868	19.339611068103267
20	24.715365644208113	26.886514291999276	31.080244171732424	17.31787589206019
21	21.791777182718462	28.61238372019239	31.791734879923517	17.80410421716563
22	22.084808643307063	25.345634986103533	31.359527223963685	21.21002914662572
23	19.588647621948382	29.33664987245707	30.965011358300444	20.1096911472941
24	18.91840213882931	26.64720738099166	36.61243448354633	17.821955996632695
25	18.336273377582057	29.936841927146123	32.37056402316501	19.356320672106804
26	18.0359658362628	32.91297045995829	29.752993980312283	19.29806972346663
27	17.637642719054448	32.9157624444247	30.614025068636288	18.832569767884564
28	16.473385196559935	30.686785875942824	34.817780710771565	18.02204821672568
29	17.385983391922704	26.522752558261526	34.53388665389122	21.557377395924547
30	17.941799814713757	30.377933170044546	32.83894056880338	18.841326446438316
31	21.942121315955347	29.465292671886832	29.94788295662694	18.64470305553088
32	22.250931719058677	28.78705196052303	30.339776049003557	18.622240271414732
33	20.75218599692882	29.78802069452729	30.318243926376216	19.141549382167682
34	18.43864614135056	29.55315557698897	30.509283348350824	21.498914933309642
35	18.42430549386398	28.846783506986306	31.84516330993989	20.883747689209827
36	21.97795178327432	29.85841254531687	28.696608584929205	19.467027086479604
37	19.13275040081898	31.427550118236315	31.043779162489265	18.395920318455442
38	18.61301826211658	31.184520561273484	30.240237572496415	19.962223604113525
39	18.890313082985394	32.377247864766424	29.18076407308231	19.551674979165874
40	21.288162408890354	29.285717307342495	29.130296838712134	20.295823445055014
41	17.177007584891133	29.009268542372595	31.212778828297182	22.600945044439086
42	19.366938673638167	29.061597099720377	28.99458947252645	22.576874754115003
43	19.725116438443088	29.78916286999082	29.06696955467848	21.418751136887614
44	18.331662372932982	30.930534580419728	29.609714413831323	21.12808863281597
45	17.616195202016996	32.840082744266915	27.998993193480292	21.544728860235796
46	19.33923034294876	31.93048804734529	28.363347166347282	20.366934443358673
47	18.96337000985655	29.919074753268948	29.991370229831084	21.126185007043414
48	19.080168026701525	28.567500454755045	31.851424123591848	20.500907394951582
49	18.71627938457894	29.01108756255526	30.90587205096641	21.366761001899395
50	17.74538793778105	30.99889589705192	30.566265213142636	20.689450952024398
51	16.990494561975712	30.999615044565992	29.855451349670677	22.154439043787626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1866.0
1	2734.5
2	3603.0
3	23825.5
4	44048.0
5	28950.0
6	13852.0
7	13889.0
8	13926.0
9	15142.5
10	16359.0
11	17249.0
12	18139.0
13	18075.0
14	18011.0
15	17882.5
16	17754.0
17	17604.5
18	17455.0
19	17555.5
20	17656.0
21	17813.5
22	17971.0
23	19306.5
24	20642.0
25	23065.5
26	31915.5
27	38342.0
28	44183.0
29	50024.0
30	56310.5
31	62597.0
32	73235.0
33	83873.0
34	92787.0
35	101701.0
36	103905.0
37	106109.0
38	117323.0
39	128537.0
40	142527.0
41	156517.0
42	168855.5
43	181194.0
44	184861.5
45	188529.0
46	183042.0
47	177555.0
48	173821.0
49	170087.0
50	159675.0
51	149263.0
52	138570.0
53	127877.0
54	114830.0
55	101783.0
56	90548.5
57	79314.0
58	74796.5
59	70279.0
60	62686.5
61	55094.0
62	47056.5
63	39019.0
64	30365.0
65	21711.0
66	17002.5
67	12294.0
68	9540.5
69	6787.0
70	5015.5
71	3244.0
72	2909.5
73	2575.0
74	1905.5
75	947.5
76	659.0
77	604.5
78	550.0
79	376.0
80	202.0
81	155.5
82	109.0
83	80.0
84	51.0
85	31.0
86	11.0
87	11.0
88	11.0
89	6.5
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2363910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.851525408849405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.10061093571383	17.503205351261116
2	7.440020632564555	3.2515159978969637
3	2.682781451357157	1.7586860115216238
4	1.3921542761410113	1.2168277815253463
5	0.8906625491553704	0.973116766178958
6	0.6306659332975192	0.8268607599567879
7	0.4574077497201022	0.6996539945649408
8	0.3509899604006135	0.6135732830356041
9	0.28319032585897974	0.5569326540943054
>10	3.3612526659754978	17.73760406605159
>50	1.402958866754602	22.38377414895751
>100	1.0045764156831263	30.603832314002986
>500	0.001948740984022549	0.2682726677041
>1k	5.846222952067647E-4	0.2587766669266374
>5k	0.0	0.0
>10k+	1.948740984022549E-4	1.3473675363214965
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31641	1.3385027348756933	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2649	0.1120601038110588	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.230279494566206E-5	0.0	0.0	0.031050251490115952	0.0
2	4.230279494566206E-5	0.0	0.0	0.10863357742046018	0.0
3	4.230279494566206E-5	0.0	0.0	0.16887275742308294	0.0
4	4.230279494566206E-5	0.0	0.0	0.30043444970409194	0.0
5	4.230279494566206E-5	0.0	0.0	0.5448176961051817	0.0
6	4.230279494566206E-5	0.0	0.0	0.8931812124827088	0.0
7	4.230279494566206E-5	0.0	0.0	1.0661150382205753	0.0
8	4.230279494566206E-5	0.0	0.0	1.4044527921959804	0.0
9	4.230279494566206E-5	0.0	0.0	1.5375796878899788	0.0
10	4.230279494566206E-5	0.0	0.0	1.758611791481063	0.0
11	4.230279494566206E-5	0.0	0.0	2.0510087101454793	0.0
12	4.230279494566206E-5	0.0	0.0	2.274198256278792	0.0
13	4.230279494566206E-5	0.0	0.0	2.3809705107216432	0.0
14	4.230279494566206E-5	0.0	0.0	2.4294918165243176	0.0
15	4.230279494566206E-5	0.0	0.0	2.4878696735493313	0.0
16	4.230279494566206E-5	0.0	0.0	2.6115630459704473	0.0
17	4.230279494566206E-5	0.0	0.0	2.766433578266516	0.0
18	4.230279494566206E-5	0.0	0.0	2.946474273555254	0.0
19	4.230279494566206E-5	0.0	0.0	3.0669526335604993	0.0
20	4.230279494566206E-5	0.0	0.0	3.18328531966107	0.0
21	4.230279494566206E-5	0.0	0.0	3.349112275848065	0.0
22	4.230279494566206E-5	0.0	0.0	3.534779242864576	0.0
23	4.230279494566206E-5	0.0	0.0	3.7311488170023392	0.0
24	4.230279494566206E-5	0.0	0.0	3.8788278741576456	0.0
25	4.230279494566206E-5	0.0	0.0	4.012420100596047	0.0
26	4.230279494566206E-5	0.0	0.0	4.13213701029227	0.0
27	4.230279494566206E-5	0.0	0.0	4.25676104420219	0.0
28	4.230279494566206E-5	0.0	0.0	4.391622354488961	0.0
29	4.230279494566206E-5	0.0	0.0	4.542304910085409	0.0
30	4.230279494566206E-5	0.0	0.0	4.720780401961157	0.0
31	8.460558989132412E-5	4.230279494566206E-5	0.0	4.881996353499075	0.0
32	8.460558989132412E-5	4.230279494566206E-5	0.0	5.035428590766992	0.0
33	8.460558989132412E-5	4.230279494566206E-5	0.0	5.183996006616157	0.0
34	8.460558989132412E-5	4.230279494566206E-5	0.0	5.338020483013313	0.0
35	8.460558989132412E-5	4.230279494566206E-5	0.0	5.522799091335965	0.0
36	8.460558989132412E-5	4.230279494566206E-5	0.0	5.6951406779445914	0.0
37	8.460558989132412E-5	4.230279494566206E-5	0.0	5.869343587530828	0.0
38	8.460558989132412E-5	4.230279494566206E-5	0.0	6.047903684996467	0.0
39	1.2690838483698618E-4	4.230279494566206E-5	0.0	6.232978412883739	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAGT	20	7.0348004E-4	45.000004	1
CTCGCGA	25	3.8920618E-5	45.0	26
CGATCGA	25	3.8920618E-5	45.0	10
TCGCGAC	25	3.8920618E-5	45.0	27
CGTTTTT	13030	0.0	43.08327	1
GACGGGT	180	0.0	41.250004	4
GCGATGT	350	0.0	40.5	9
TACGGGA	945	0.0	40.47619	4
TGCGTAG	145	0.0	40.344826	1
AAGGGCG	720	0.0	40.000004	5
CTCGTCC	920	0.0	39.375004	37
TCGCGAG	40	3.4602817E-7	39.375004	1
CGTATGG	335	0.0	38.955223	2
TAAGGGA	3675	0.0	38.693874	4
ACGGGAT	1355	0.0	38.690037	5
GGGCGAT	3395	0.0	38.57143	7
TCGATAG	135	0.0	38.333332	1
TCGTCCC	935	0.0	38.26203	38
TATGGGA	2880	0.0	38.20313	4
GGTCGGA	65	9.094947E-12	38.07692	8
>>END_MODULE