FastQCFastQC Report
Thu 26 May 2016
SRR1547422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547422_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2638789
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT673842.55359560768216No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCCATGAATCGTATGCCGTCTT27040.10247124722742137No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT327050.044.0024451
CGACGGT1500.040.528
GTTAGCG1150.039.1304321
GGCCGAT6000.039.08
CGTAAGG4450.038.9325872
AGGGCGA19650.038.9312976
GTTTTTT375100.038.6656882
TACGCGG1400.038.5714262
CGGTCTA1400.038.57142631
GGGCGAT39800.038.3291477
AGGGATT50600.038.0187726
ATAGGGA41100.037.9927024
TAGGGAT41250.037.963645
CGAGGGT7850.037.8343964
GACCGAT13800.037.59
TAGGGAC31400.037.3328065
AAGGGAT44950.037.2914355
TATGGGT10200.037.279414
GCGTAAG1450.037.2413831
TTAGGGA39250.037.0891764