##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547422_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2638789
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29023654411171	31.0	31.0	33.0	30.0	34.0
2	31.68688629519071	31.0	31.0	34.0	30.0	34.0
3	31.609656171827304	31.0	31.0	34.0	30.0	34.0
4	35.492167050870684	37.0	35.0	37.0	33.0	37.0
5	35.438070266322924	37.0	35.0	37.0	33.0	37.0
6	35.497552854737535	37.0	35.0	37.0	33.0	37.0
7	35.95029992924785	37.0	35.0	37.0	35.0	37.0
8	35.94813075240195	37.0	35.0	37.0	35.0	37.0
9	37.664879609548166	39.0	37.0	39.0	35.0	39.0
10	36.90948651066834	39.0	37.0	39.0	32.0	39.0
11	36.65670313162591	39.0	35.0	39.0	32.0	39.0
12	36.50422978116098	39.0	35.0	39.0	32.0	39.0
13	36.43899190120923	39.0	35.0	39.0	32.0	39.0
14	37.60165287940794	40.0	36.0	41.0	32.0	41.0
15	37.698973279030646	40.0	36.0	41.0	32.0	41.0
16	37.728680845645485	40.0	36.0	41.0	32.0	41.0
17	37.63661323432832	40.0	36.0	41.0	32.0	41.0
18	37.4008122665359	39.0	36.0	41.0	32.0	41.0
19	37.21344146879497	38.0	36.0	41.0	32.0	41.0
20	36.93426075370179	38.0	35.0	41.0	32.0	41.0
21	36.82389194437297	38.0	35.0	40.0	31.0	41.0
22	36.75044840644705	38.0	35.0	40.0	31.0	41.0
23	36.641730354340574	38.0	35.0	40.0	31.0	41.0
24	36.49609195733346	38.0	35.0	40.0	31.0	41.0
25	36.402833648313674	38.0	35.0	40.0	31.0	41.0
26	36.245368993125254	38.0	35.0	40.0	30.0	41.0
27	36.1494624996542	38.0	35.0	40.0	30.0	41.0
28	36.13525295125908	38.0	35.0	40.0	30.0	41.0
29	36.09951458794166	38.0	35.0	40.0	30.0	41.0
30	35.9851640278931	38.0	35.0	40.0	30.0	41.0
31	35.79780194627157	38.0	35.0	40.0	29.0	41.0
32	35.528012660352914	38.0	34.0	40.0	27.0	41.0
33	35.07456602252018	38.0	34.0	40.0	24.0	41.0
34	34.59386028970107	38.0	34.0	40.0	22.0	41.0
35	34.23607078853217	38.0	33.0	40.0	20.0	41.0
36	33.995482018456194	38.0	33.0	40.0	18.0	41.0
37	33.862970097268104	38.0	33.0	40.0	18.0	41.0
38	33.764114523745555	38.0	33.0	40.0	18.0	41.0
39	33.70808806615459	37.0	33.0	40.0	17.0	41.0
40	33.57567656982047	37.0	33.0	40.0	16.0	41.0
41	33.47070720698017	37.0	33.0	40.0	15.0	41.0
42	33.37818711537755	37.0	33.0	40.0	15.0	41.0
43	33.32503773511258	37.0	33.0	40.0	15.0	41.0
44	33.19885030595474	37.0	32.0	40.0	15.0	41.0
45	33.11278469024996	37.0	32.0	40.0	15.0	41.0
46	33.036001741708034	37.0	32.0	40.0	15.0	41.0
47	32.90252308918978	36.0	32.0	40.0	14.0	41.0
48	32.85091949375263	36.0	32.0	40.0	14.0	41.0
49	32.73525317863611	36.0	31.0	40.0	13.0	41.0
50	32.649879925981196	36.0	31.0	40.0	13.0	41.0
51	32.26265912128632	35.0	31.0	39.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	4.0
13	9.0
14	24.0
15	86.0
16	316.0
17	783.0
18	1766.0
19	3371.0
20	5917.0
21	9340.0
22	14113.0
23	20718.0
24	31401.0
25	48438.0
26	69608.0
27	76934.0
28	74226.0
29	71329.0
30	74985.0
31	83631.0
32	98809.0
33	121600.0
34	172211.0
35	233742.0
36	224124.0
37	256799.0
38	363927.0
39	580428.0
40	148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.50311677060955	23.006310849408575	25.083021037301577	22.4075513426803
2	37.52850265784797	23.970313655241096	26.635096629552418	11.866087057358508
3	25.352652296185862	23.73524370459328	38.86354687699547	12.048557122225384
4	21.57580617472636	26.513866777525603	38.94195405543982	12.968372992308213
5	20.49125564795063	30.16758823839269	36.98689057745807	12.354265536198612
6	19.129494628028233	38.25266059544738	33.523711065947296	9.094133710577086
7	82.32477094606655	2.308710548664558	14.398309224420746	0.9682092808481466
8	82.92648635415716	1.9159167330165465	14.130989631986491	1.0266072808398095
9	78.39599907381756	4.619808556121766	15.384746563669927	1.5994458063907344
10	31.887733350411875	33.83840087252145	25.058085356578342	9.215780420488336
11	23.504456021303714	25.541185748462645	35.123952691935585	15.83040553829806
12	23.670289666964656	23.22792765924066	35.98988020641286	17.111902467381817
13	20.331030635643852	26.306082070222363	36.93561705767305	16.42727023646074
14	17.164843418704564	28.909928001064124	36.534145018794604	17.391083561436705
15	16.792665120250234	27.62373194673769	39.988267345361834	15.595335587650244
16	19.544419807722406	26.159272302559998	38.524641416953	15.77166647276459
17	19.28464155337922	26.429699381041832	35.48476213899633	18.80089692658261
18	19.536537404089525	26.892411632760332	36.86255323938367	16.70849772376647
19	20.978827788049745	28.26667838921566	34.089917761518635	16.66457606121596
20	22.20583002278697	28.04517526789751	34.18613614047959	15.562858568835933
21	21.024037920424863	27.180308846216956	36.302106761851746	15.493546471506436
22	21.01255538051735	25.009426672613838	35.26378956407655	18.714228382792257
23	17.85436425572488	28.662124936855506	35.59553264774107	17.88797815967855
24	17.66692979241614	27.747614530756344	38.26774327162952	16.31771240519799
25	18.23487971186783	29.19699149875189	35.77080244005868	16.7973263493216
26	18.141389857241332	30.74035097160099	33.978730394889475	17.139528776268204
27	17.21638221168877	30.486749793181644	35.335034366142956	16.961833628986632
28	16.820594598507117	29.691612326715024	37.13779313162212	16.349999943155744
29	17.19497087489754	27.908900635859858	36.902078946062	17.994049543180605
30	17.930990314117572	28.583604069897213	35.982566245349666	17.502839370635545
31	19.57272824769241	28.904812017937015	33.97456181604516	17.547897918325415
32	20.06382473172353	28.78706861367089	33.73445167461286	17.414654979992715
33	19.450058341155735	29.38173533389748	32.726792479429015	18.441413845517772
34	18.115961526291038	30.03669486268133	33.245022622119464	18.60232098890817
35	18.80995411152616	30.43464255762776	32.54432241456213	18.211080916283947
36	20.64769104312622	31.24592379307326	30.346533959327555	17.75985120447296
37	18.692627565144466	32.40293179939738	31.143149376475343	17.761291258982816
38	18.876461892178572	32.26048009143588	30.626321392123433	18.236736624262115
39	18.5855708811883	32.42259991230826	30.271385851616024	18.72044335488741
40	20.234622775826335	30.960982480978966	30.298026860048306	18.5063678831464
41	18.111982428303286	30.769152061798046	31.081227032551674	20.037638477346995
42	19.03585318871649	31.3366851233653	29.883594330581186	19.743867357337024
43	19.1010724995443	31.427560142171274	29.84410652007417	19.627260838210255
44	18.67887125495824	32.073576174525506	30.07140775560304	19.176144814913204
45	18.569616593065984	32.78253774742884	28.83800106791411	19.809844591591066
46	19.473932929082242	32.59233686361433	29.087395771317826	18.846334435985597
47	18.92565112254144	31.496910135672085	30.42532009948503	19.15211864230145
48	19.078865343155517	30.596156039759148	31.078650092902464	19.246328524182875
49	19.01133436587768	30.504106239642503	30.89898434471267	19.585575049767147
50	18.204411190133047	31.939916378308382	30.690896468038936	19.164775963519627
51	17.924131107110117	31.963677277720954	29.933768861398164	20.178422753770764
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1987.0
1	2644.0
2	3301.0
3	49303.0
4	95305.0
5	63700.5
6	32096.0
7	32896.0
8	33696.0
9	36401.0
10	39106.0
11	41702.5
12	44299.0
13	44623.0
14	44947.0
15	42449.5
16	39952.0
17	37704.0
18	35456.0
19	33128.0
20	30800.0
21	29901.0
22	29002.0
23	29256.0
24	29510.0
25	31436.5
26	36054.5
27	38746.0
28	45753.0
29	52760.0
30	57752.5
31	62745.0
32	70556.0
33	78367.0
34	87130.0
35	95893.0
36	102192.5
37	108492.0
38	115876.5
39	123261.0
40	136744.5
41	150228.0
42	158818.0
43	167408.0
44	171366.0
45	175324.0
46	180111.0
47	184898.0
48	181099.5
49	177301.0
50	169658.0
51	162015.0
52	150025.0
53	138035.0
54	124735.0
55	111435.0
56	102576.0
57	93717.0
58	83248.0
59	72779.0
60	64076.5
61	55374.0
62	46387.5
63	37401.0
64	31349.0
65	25297.0
66	19691.0
67	14085.0
68	10844.5
69	7604.0
70	6209.5
71	4815.0
72	3895.5
73	2976.0
74	2705.0
75	1803.0
76	1172.0
77	972.0
78	772.0
79	530.5
80	289.0
81	220.5
82	152.0
83	118.5
84	85.0
85	74.0
86	63.0
87	45.5
88	28.0
89	19.0
90	10.0
91	8.0
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2638789.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.593153949535413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.79027111735562	20.68688655599936
2	6.947270863014389	3.694996871785268
3	3.0396725224098766	2.425034380338555
4	1.8888983367707672	2.009270570590656
5	1.3264630791162029	1.7637418435655983
6	1.0096586318963063	1.6110004460697473
7	0.7337997206902668	1.3659834258309647
8	0.5648431350529147	1.2016768358240308
9	0.45371273380340793	1.0859087321008747
>10	4.787559790754808	28.782510142095987
>50	1.146231233836272	21.42484735650117
>100	0.30858268072142564	10.596663893179793
>500	0.002024103051711613	0.36675604477532836
>1k	8.67472736447834E-4	0.3939388232275935
>5k	0.0	0.0
>10k+	1.4457878940797234E-4	2.5907840781151825
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67384	2.55359560768216	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCCATGAATCGTATGCCGTCTT	2704	0.10247124722742137	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1368851393574856E-4	0.0	0.0	0.02565570797816726	0.0
2	1.1368851393574856E-4	0.0	0.0	0.06290764437778087	0.0
3	1.1368851393574856E-4	0.0	0.0	0.11459802204723454	0.0
4	1.1368851393574856E-4	0.0	0.0	0.1870555015956183	0.0
5	1.1368851393574856E-4	0.0	0.0	0.3736562491354936	0.0
6	1.1368851393574856E-4	0.0	0.0	0.7375352860725128	0.0
7	1.1368851393574856E-4	0.0	0.0	0.9779485968753091	0.0
8	1.1368851393574856E-4	0.0	0.0	1.318483592284188	0.0
9	1.5158468524766475E-4	0.0	0.0	1.4878794780484532	0.0
10	1.5158468524766475E-4	0.0	0.0	1.683385825846629	0.0
11	1.5158468524766475E-4	0.0	0.0	1.8802564358120335	0.0
12	1.5158468524766475E-4	0.0	0.0	2.039306666808146	0.0
13	1.5158468524766475E-4	0.0	0.0	2.1415505370076957	0.0
14	1.5158468524766475E-4	0.0	0.0	2.1979779360911387	0.0
15	1.5158468524766475E-4	0.0	0.0	2.248683013306483	0.0
16	1.5158468524766475E-4	0.0	0.0	2.3295155467148	0.0
17	1.5158468524766475E-4	0.0	0.0	2.423005401341297	0.0
18	1.5158468524766475E-4	0.0	0.0	2.5281293805605527	0.0
19	1.5158468524766475E-4	0.0	0.0	2.6063091819770356	0.0
20	1.5158468524766475E-4	0.0	0.0	2.6883922890386462	0.0
21	1.5158468524766475E-4	0.0	0.0	2.7900298204972054	0.0
22	1.5158468524766475E-4	0.0	0.0	2.912396557663383	0.0
23	1.5158468524766475E-4	0.0	0.0	3.050528102095317	0.0
24	1.5158468524766475E-4	0.0	0.0	3.163269211748268	0.0
25	1.8948085655958092E-4	0.0	0.0	3.264868847035515	0.0
26	1.8948085655958092E-4	3.789617131191619E-5	0.0	3.362565176677635	0.0
27	1.8948085655958092E-4	3.789617131191619E-5	0.0	3.466552270757533	0.0
28	1.8948085655958092E-4	3.789617131191619E-5	0.0	3.5748595283669897	0.0
29	1.8948085655958092E-4	3.789617131191619E-5	0.0	3.6953314569675713	0.0
30	1.8948085655958092E-4	3.789617131191619E-5	0.0	3.8408527548053293	0.0
31	1.8948085655958092E-4	3.789617131191619E-5	0.0	3.972655638628174	0.0
32	1.8948085655958092E-4	3.789617131191619E-5	0.0	4.108513412781393	0.0
33	1.8948085655958092E-4	3.789617131191619E-5	0.0	4.2390657229509445	0.0
34	1.8948085655958092E-4	3.789617131191619E-5	0.0	4.369390656092624	0.0
35	1.8948085655958092E-4	3.789617131191619E-5	0.0	4.5223774996788295	0.0
36	1.8948085655958092E-4	3.789617131191619E-5	0.0	4.660509044110764	0.0
37	1.8948085655958092E-4	3.789617131191619E-5	0.0	4.808076735199366	0.0
38	1.8948085655958092E-4	3.789617131191619E-5	0.0	4.965270053801194	0.0
39	1.8948085655958092E-4	3.789617131191619E-5	0.0	5.167370335407643	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	32705	0.0	44.002445	1
CGACGGT	150	0.0	40.5	28
GTTAGCG	115	0.0	39.130432	1
GGCCGAT	600	0.0	39.0	8
CGTAAGG	445	0.0	38.932587	2
AGGGCGA	1965	0.0	38.931297	6
GTTTTTT	37510	0.0	38.665688	2
TACGCGG	140	0.0	38.571426	2
CGGTCTA	140	0.0	38.571426	31
GGGCGAT	3980	0.0	38.329147	7
AGGGATT	5060	0.0	38.018772	6
ATAGGGA	4110	0.0	37.992702	4
TAGGGAT	4125	0.0	37.96364	5
CGAGGGT	785	0.0	37.834396	4
GACCGAT	1380	0.0	37.5	9
TAGGGAC	3140	0.0	37.332806	5
AAGGGAT	4495	0.0	37.291435	5
TATGGGT	1020	0.0	37.27941	4
GCGTAAG	145	0.0	37.241383	1
TTAGGGA	3925	0.0	37.089176	4
>>END_MODULE