FastQCFastQC Report
Thu 26 May 2016
SRR1547421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547421_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1046017
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122731.1733078907895378No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC19990.19110588068836357No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11000.10516081478599297No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGA453.8562575E-1045.00000412
AACGGGA3100.045.0000044
ATCGGCC453.8562575E-1045.00000435
TATCGTG453.8562575E-1045.0000041
GTCGGAC453.8562575E-1045.00000410
ACGCGAC207.0329965E-445.036
TCACGCG253.890564E-545.034
CGTGGTA351.2117926E-745.022
TATCACG253.890564E-545.01
AACCGGA207.0329965E-445.010
CTGTCGT207.0329965E-445.044
AATCCGA207.0329965E-445.026
TCGTTCA253.890564E-545.016
GCCCATA253.890564E-545.013
CTCGTTA207.0329965E-445.045
GTCGATC207.0329965E-445.045
CAAGTAG1400.045.01
GGTCGAC351.2117926E-745.08
ACGTTAC207.0329965E-445.012
CGTGCCA207.0329965E-445.037