FastQCFastQC Report
Thu 26 May 2016
SRR1547420_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547420_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1411027
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171461.21514329633664No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24550.1739867486589555No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15950.11303823385378167No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT14150.1002815679643267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT302.1658543E-645.00000438
ACTCTCG302.1658543E-645.00000441
GTCAACC302.1658543E-645.00000433
CGTACTC302.1658543E-645.00000430
TATAACG600.045.0000041
TGTCGCG302.1658543E-645.00000424
GTCGGAT302.1658543E-645.00000421
TATCCGA302.1658543E-645.00000413
TGATCGC207.0338335E-445.019
CGAACGC207.0338335E-445.024
GATCGAC207.0338335E-445.08
CGTTGCG207.0338335E-445.031
GCACGAG406.8175723E-945.01
CGCGATG406.8175723E-945.022
ACCTCGC207.0338335E-445.033
CGTTATG207.0338335E-445.029
TTATACG406.8175723E-945.01
ATCCGCC351.2121927E-745.019
CGTAGGT406.8175723E-945.03
GTGTCGA207.0338335E-445.023