##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547420_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1411027
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44550742119038	31.0	31.0	33.0	30.0	34.0
2	31.863432804616778	31.0	31.0	34.0	30.0	34.0
3	31.92623954041985	31.0	31.0	34.0	30.0	34.0
4	35.70032040492492	37.0	35.0	37.0	33.0	37.0
5	35.613048510056856	37.0	35.0	37.0	33.0	37.0
6	35.64624064599756	37.0	35.0	37.0	33.0	37.0
7	35.99673216742132	37.0	35.0	37.0	35.0	37.0
8	35.9821668897902	37.0	35.0	37.0	35.0	37.0
9	37.715679430655825	39.0	38.0	39.0	35.0	39.0
10	37.131353971256395	39.0	37.0	39.0	33.0	39.0
11	36.792052171928674	39.0	37.0	39.0	32.0	39.0
12	36.12460640370453	38.0	35.0	39.0	32.0	39.0
13	35.726672841837896	38.0	35.0	39.0	31.0	39.0
14	36.81953003025456	39.0	35.0	41.0	31.0	41.0
15	37.089332805112875	39.0	35.0	41.0	32.0	41.0
16	37.255931318110854	39.0	35.0	41.0	32.0	41.0
17	37.170036434455184	39.0	35.0	41.0	32.0	41.0
18	37.02557924121934	38.0	35.0	40.0	32.0	41.0
19	36.91832544664276	38.0	35.0	40.0	32.0	41.0
20	36.68624342411591	38.0	35.0	40.0	32.0	41.0
21	36.558249416914066	38.0	35.0	40.0	31.0	41.0
22	36.46677420063542	38.0	35.0	40.0	31.0	41.0
23	36.40596317434039	38.0	35.0	40.0	31.0	41.0
24	36.26599278397933	38.0	35.0	40.0	31.0	41.0
25	36.188786607201706	37.0	35.0	40.0	31.0	41.0
26	36.05924479120527	37.0	35.0	40.0	31.0	41.0
27	35.96420266940321	37.0	35.0	40.0	30.0	41.0
28	36.063243297257955	37.0	35.0	40.0	30.0	41.0
29	36.11230826908344	37.0	35.0	40.0	31.0	41.0
30	36.06729495608518	37.0	35.0	40.0	30.0	41.0
31	35.90246607612753	37.0	35.0	40.0	30.0	41.0
32	35.702319657951264	37.0	34.0	40.0	30.0	41.0
33	35.43483930498849	37.0	34.0	40.0	29.0	41.0
34	35.15465543891081	37.0	34.0	40.0	27.0	41.0
35	34.952776948988216	37.0	34.0	40.0	25.0	41.0
36	34.73588953294303	37.0	34.0	40.0	24.0	41.0
37	34.62395049846672	37.0	34.0	40.0	24.0	41.0
38	34.572608461779964	37.0	34.0	40.0	23.0	41.0
39	34.556611602754586	37.0	34.0	40.0	23.0	41.0
40	34.365785346417894	37.0	33.0	40.0	23.0	41.0
41	34.301728457357655	36.0	34.0	40.0	23.0	41.0
42	34.23256535842333	36.0	33.0	40.0	23.0	41.0
43	34.164919594026195	36.0	33.0	40.0	23.0	41.0
44	34.0047773713756	36.0	33.0	40.0	23.0	41.0
45	33.91651187397548	36.0	33.0	40.0	23.0	41.0
46	33.83609810443032	36.0	33.0	40.0	22.0	41.0
47	33.74138765594138	35.0	33.0	40.0	22.0	41.0
48	33.729226301126765	35.0	33.0	40.0	22.0	41.0
49	33.63312395864856	36.0	33.0	40.0	22.0	41.0
50	33.56144354431205	35.0	33.0	40.0	21.0	41.0
51	33.124386705569776	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	2.0
13	7.0
14	29.0
15	59.0
16	145.0
17	320.0
18	749.0
19	1397.0
20	2481.0
21	3892.0
22	5893.0
23	8874.0
24	12670.0
25	19012.0
26	26326.0
27	31194.0
28	32760.0
29	34174.0
30	38885.0
31	45759.0
32	55702.0
33	72181.0
34	123620.0
35	180826.0
36	99639.0
37	122255.0
38	180269.0
39	311758.0
40	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.258901495152113	24.088341328691797	29.09788402348077	17.55487315267532
2	31.594930500975533	27.044627778206937	28.36416312373895	12.99627859707858
3	27.869913190888624	25.1579877635226	34.0247918714525	12.947307174136286
4	24.353467368094304	27.003452095530417	33.53082542006638	15.112255116308901
5	22.526712812724348	32.23007072153828	31.786847452245777	13.456369013491592
6	22.178597574674335	40.064931429377324	27.989754979883447	9.766716016064894
7	86.50727448872347	3.718213754945866	8.802453815554202	0.9720579407764699
8	87.28975420030942	2.632196265556931	8.659579157592306	1.4184703765413418
9	82.55086543347505	5.71938028117109	9.814766124248509	1.914988161105351
10	43.93083902717666	33.16378779428034	14.583278704092834	8.32209447445017
11	38.73448204747322	20.502584287898102	27.008342150788046	13.754591513840628
12	39.42327113513774	18.961295566987733	27.368221869602777	14.247211428271747
13	22.145288502629647	33.31941911813169	27.13300312467444	17.40228925456423
14	15.708700117006973	35.937299569746	29.585259530824004	18.768740782423016
15	14.957049014653863	26.508209977555353	43.527657514703826	15.00708349308695
16	19.06412846812995	21.605256313309383	42.5637496660234	16.766865552537265
17	18.02913764229884	22.52607497942988	30.9436318369528	28.501155541318486
18	20.345322945627547	25.41808200693537	35.033773272942334	19.202821774494748
19	25.761023708263554	25.568681534796994	28.97251434593385	19.6977804110056
20	27.681964980117318	24.00818694468639	30.22110845504728	18.08873962014901
21	23.32301224569055	27.10472584861948	31.116839011585178	18.45542289410479
22	24.04943349772896	23.97261002092802	29.525941034438034	22.452015446904987
23	20.568989820889325	28.40080310298811	29.221836293706644	21.808370782415928
24	20.465235604988425	24.18068541565824	38.1043736229002	17.249705356453138
25	18.264498127959282	25.774063855617218	35.526605798471614	20.43483221795189
26	18.317863513596837	31.79988759959944	30.09545529603615	19.786793590767576
27	18.22743292651381	33.1418888511701	31.07204894023998	17.558629282076105
28	15.647113768907328	29.516373535020946	37.35173033542235	17.48478236064937
29	16.762613330574112	24.74679789968583	37.29595535733902	21.194633412401036
30	18.304539884778958	29.68837591343043	33.162937349887706	18.844146851902906
31	24.889459946549568	27.347385981983336	28.628722200213037	19.13443187125406
32	25.476904410758973	26.504241237056412	30.34392679941631	17.674927552768303
33	24.339718517080115	28.85125514961797	28.219091484429427	18.58993484887249
34	19.60557806477126	28.305836812477718	30.026144078036776	22.06244104471424
35	19.828252754908306	26.988073226097022	31.846165948631743	21.337508070362933
36	26.131250500521958	26.84874208643775	28.390101677714174	18.629905735326112
37	20.269775135415554	31.22895593068028	30.868650989669227	17.632617944234944
38	20.17140706733464	29.774058185987933	28.393432584918642	21.661102161758773
39	20.14157064322653	29.569880661390602	30.012962189951008	20.275586505431857
40	23.282403525942453	26.589781768881814	28.465295136095907	21.662519569079826
41	16.74347833173993	26.226642013228663	31.59634790829658	25.43353174673483
42	20.573809005780895	26.573127232859473	27.832281026514732	25.020782734844904
43	21.396543085284687	27.22109498967773	28.049427828099677	23.332934096937905
44	19.523014088320068	29.806020721077626	28.9752782902099	21.69568690039241
45	19.51124960755535	33.237705586073126	25.277546071053216	21.973498735318316
46	21.09378488150829	31.851197744621473	27.35737870359674	19.697638670273495
47	20.63057616898897	28.012575237752362	29.411768874727418	21.945079718531254
48	22.18660592603827	25.002356439671246	32.26260021955639	20.548437414734092
49	20.351488667474115	26.015802674222392	31.865584428930134	21.76712422937336
50	19.729034242434764	29.159612112312523	30.242582175961196	20.868771469291516
51	17.898665298396132	30.214234029540187	27.840572859342878	24.046527812720804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1686.0
1	2482.5
2	3279.0
3	14891.5
4	26504.0
5	18368.0
6	10232.0
7	10151.0
8	10070.0
9	10538.0
10	11006.0
11	11057.0
12	11108.0
13	11009.5
14	10911.0
15	10350.0
16	9789.0
17	9096.5
18	8404.0
19	8482.5
20	8561.0
21	8699.0
22	8837.0
23	8995.5
24	9154.0
25	10088.0
26	13068.0
27	15114.0
28	19197.5
29	23281.0
30	26255.0
31	29229.0
32	34884.0
33	40539.0
34	43571.5
35	46604.0
36	47354.5
37	48105.0
38	51214.5
39	54324.0
40	71741.0
41	89158.0
42	101735.0
43	114312.0
44	117597.0
45	120882.0
46	114584.0
47	108286.0
48	102836.5
49	97387.0
50	91279.0
51	85171.0
52	80497.0
53	75823.0
54	68967.5
55	62112.0
56	62313.5
57	62515.0
58	59808.5
59	57102.0
60	53033.0
61	48964.0
62	41069.0
63	33174.0
64	27501.5
65	21829.0
66	17711.0
67	13593.0
68	10689.0
69	7785.0
70	6755.0
71	5725.0
72	5008.0
73	4291.0
74	3327.5
75	1723.0
76	1082.0
77	974.0
78	866.0
79	688.0
80	510.0
81	347.0
82	184.0
83	132.5
84	81.0
85	56.5
86	32.0
87	22.0
88	12.0
89	8.5
90	5.0
91	7.0
92	9.0
93	10.0
94	11.0
95	7.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1411027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.770803755705774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.56423732062991	15.685678997407686
2	6.867928694355934	2.578330834598716
3	1.9506422522978215	1.0984536874641095
4	0.9221584938463602	0.6923862447858895
5	0.5346524399113077	0.5017928013542214
6	0.3602242416756062	0.40570191291244395
7	0.26874158436012396	0.3531146878714934
8	0.2053128438884128	0.30831096809242026
9	0.17237155199081874	0.2911997317937484
>10	2.287165070567711	11.0434078711754
>50	1.2728066803998668	17.49043472886909
>100	1.588826937302239	47.482836863298985
>500	0.0030350084762220423	0.36802328333627654
>1k	0.0015175042381110212	0.47932753872609857
>5k	0.0	0.0
>10k+	3.793760595277553E-4	1.2209998483134283
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17146	1.21514329633664	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2455	0.1739867486589555	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1595	0.11303823385378167	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1415	0.1002815679643267	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.08703660525277E-5	0.0	0.0	0.021757202378126003	0.0
2	7.08703660525277E-5	0.0	0.0	0.08837534646750204	0.0
3	7.08703660525277E-5	0.0	0.0	0.12324356656534567	0.0
4	7.08703660525277E-5	0.0	0.0	0.23160435625966053	0.0
5	7.08703660525277E-5	0.0	0.0	0.36994331079419457	0.0
6	7.08703660525277E-5	0.0	0.0	0.5427961335963096	0.0
7	7.08703660525277E-5	0.0	0.0	0.6258562026098721	0.0
8	7.08703660525277E-5	0.0	0.0	0.8339315973400934	0.0
9	7.08703660525277E-5	0.0	0.0	0.903171944973413	0.0
10	7.08703660525277E-5	0.0	0.0	1.0567480282092405	0.0
11	7.08703660525277E-5	0.0	0.0	1.3176927160146474	0.0
12	7.08703660525277E-5	0.0	0.0	1.4962860384670174	0.0
13	7.08703660525277E-5	0.0	0.0	1.568219460010333	0.0
14	7.08703660525277E-5	0.0	0.0	1.5951501991102934	0.0
15	7.08703660525277E-5	0.0	0.0	1.6441924924186426	0.0
16	7.08703660525277E-5	0.0	0.0	1.7465293009984926	0.0
17	7.08703660525277E-5	0.0	0.0	1.8641741086456887	0.0
18	7.08703660525277E-5	0.0	0.0	2.0071905073396894	0.0
19	7.08703660525277E-5	0.0	0.0	2.082313095355369	0.0
20	7.08703660525277E-5	0.0	0.0	2.165373164368931	0.0
21	7.08703660525277E-5	0.0	0.0	2.2784822685887653	0.0
22	7.08703660525277E-5	0.0	0.0	2.3871265397472903	0.0
23	7.08703660525277E-5	0.0	0.0	2.504416995564224	0.0
24	7.08703660525277E-5	0.0	0.0	2.595131064111459	0.0
25	7.08703660525277E-5	0.0	0.0	2.674364133358185	0.0
26	7.08703660525277E-5	0.0	0.0	2.7511167397930727	0.0
27	7.08703660525277E-5	0.0	0.0	2.8248219204877016	0.0
28	7.08703660525277E-5	0.0	0.0	2.900865823262064	0.0
29	2.1261109815758308E-4	0.0	0.0	2.985059818132467	0.0
30	2.1261109815758308E-4	0.0	0.0	3.0863335712215285	0.0
31	2.1261109815758308E-4	0.0	0.0	3.1939856572553182	0.0
32	2.1261109815758308E-4	0.0	0.0	3.2871093182483397	0.0
33	2.1261109815758308E-4	0.0	0.0	3.379666016312941	0.0
34	2.1261109815758308E-4	0.0	0.0	3.4794514917149	0.0
35	2.1261109815758308E-4	0.0	0.0	3.6110577614744437	0.0
36	2.1261109815758308E-4	0.0	0.0	3.716229384696395	7.08703660525277E-5
37	2.1261109815758308E-4	0.0	0.0	3.8313937295317526	7.08703660525277E-5
38	2.1261109815758308E-4	0.0	0.0	3.9374866675123865	7.08703660525277E-5
39	2.1261109815758308E-4	0.0	0.0	4.0595963082208915	7.08703660525277E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACCGT	30	2.1658543E-6	45.000004	38
ACTCTCG	30	2.1658543E-6	45.000004	41
GTCAACC	30	2.1658543E-6	45.000004	33
CGTACTC	30	2.1658543E-6	45.000004	30
TATAACG	60	0.0	45.000004	1
TGTCGCG	30	2.1658543E-6	45.000004	24
GTCGGAT	30	2.1658543E-6	45.000004	21
TATCCGA	30	2.1658543E-6	45.000004	13
TGATCGC	20	7.0338335E-4	45.0	19
CGAACGC	20	7.0338335E-4	45.0	24
GATCGAC	20	7.0338335E-4	45.0	8
CGTTGCG	20	7.0338335E-4	45.0	31
GCACGAG	40	6.8175723E-9	45.0	1
CGCGATG	40	6.8175723E-9	45.0	22
ACCTCGC	20	7.0338335E-4	45.0	33
CGTTATG	20	7.0338335E-4	45.0	29
TTATACG	40	6.8175723E-9	45.0	1
ATCCGCC	35	1.2121927E-7	45.0	19
CGTAGGT	40	6.8175723E-9	45.0	3
GTGTCGA	20	7.0338335E-4	45.0	23
>>END_MODULE