FastQCFastQC Report
Thu 26 May 2016
SRR1547419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547419_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3169662
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT589871.8609870705456923No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG900.045.01
CGTATCG207.035166E-445.041
ATAGCGC253.8923645E-545.015
CGCCGAT207.035166E-445.015
CGTTTTT321300.044.1456571
TACGGGT9300.041.129034
TAGGGTA37700.039.6883285
ACGGGTA7500.039.35
GTTAGCG1500.039.01
TATAGCG2250.039.01
AGGGTAC25450.038.6345756
GTTTTTT371350.038.6017272
TAGGGCG9300.038.467745
CGTTAGG3300.038.181822
CGTTCGA659.094947E-1238.07692714
AGGGCGA23100.037.9870156
TTAGGGT35000.037.928574
GGGCGAT44100.037.8061267
GCGATGC3100.037.7419369
ATAGGGT34950.037.532194
Produced by FastQC (version 0.11.5)