##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547419_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3169662
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.270439561063608	31.0	31.0	33.0	30.0	34.0
2	31.676480331341324	31.0	31.0	34.0	30.0	34.0
3	31.674865017153248	31.0	31.0	34.0	30.0	34.0
4	35.5400276117769	37.0	35.0	37.0	33.0	37.0
5	35.46674030227829	37.0	35.0	37.0	33.0	37.0
6	35.520520169027485	37.0	35.0	37.0	33.0	37.0
7	35.95415820361919	37.0	35.0	37.0	35.0	37.0
8	35.95319784885581	37.0	35.0	37.0	35.0	37.0
9	37.66066791979713	39.0	37.0	39.0	35.0	39.0
10	36.68365396688984	39.0	37.0	39.0	32.0	39.0
11	36.61533501048377	39.0	35.0	39.0	32.0	39.0
12	36.42780428954254	39.0	35.0	39.0	32.0	39.0
13	36.323242667514705	39.0	35.0	39.0	32.0	39.0
14	37.438987185384434	39.0	36.0	41.0	32.0	41.0
15	37.55409157190893	40.0	36.0	41.0	32.0	41.0
16	37.60425559570705	40.0	36.0	41.0	32.0	41.0
17	37.524434466514094	39.0	36.0	41.0	32.0	41.0
18	37.32884925900616	39.0	36.0	41.0	32.0	41.0
19	37.18527464442581	38.0	36.0	41.0	32.0	41.0
20	36.949235912220296	38.0	35.0	41.0	32.0	41.0
21	36.82163776453136	38.0	35.0	40.0	31.0	41.0
22	36.74693926355555	38.0	35.0	40.0	31.0	41.0
23	36.6575066994525	38.0	35.0	40.0	31.0	41.0
24	36.50665402178529	38.0	35.0	40.0	31.0	41.0
25	36.415259103336574	38.0	35.0	40.0	31.0	41.0
26	36.29008171849238	38.0	35.0	40.0	30.0	41.0
27	36.20023933151232	38.0	35.0	40.0	30.0	41.0
28	36.242901293576416	38.0	35.0	40.0	30.0	41.0
29	36.223877814101314	38.0	35.0	40.0	30.0	41.0
30	36.12707443254202	38.0	35.0	40.0	30.0	41.0
31	35.974817188709714	38.0	35.0	40.0	30.0	41.0
32	35.741872477254674	38.0	35.0	40.0	29.0	41.0
33	35.36470292416037	38.0	34.0	40.0	26.0	41.0
34	34.953142006939544	38.0	34.0	40.0	24.0	41.0
35	34.648464410400855	38.0	34.0	40.0	23.0	41.0
36	34.43499937848263	38.0	33.0	40.0	21.0	41.0
37	34.33211301394281	38.0	33.0	40.0	21.0	41.0
38	34.23655298262086	38.0	33.0	40.0	21.0	41.0
39	34.19368689784589	38.0	33.0	40.0	20.0	41.0
40	34.044374447496295	38.0	33.0	40.0	19.0	41.0
41	33.959763217655386	38.0	33.0	40.0	18.0	41.0
42	33.88308627229023	37.0	33.0	40.0	18.0	41.0
43	33.82870444861313	37.0	33.0	40.0	18.0	41.0
44	33.71206614459207	37.0	33.0	40.0	18.0	41.0
45	33.60961168730294	37.0	33.0	40.0	18.0	41.0
46	33.52632488889983	37.0	33.0	40.0	18.0	41.0
47	33.38486248691501	37.0	32.0	40.0	18.0	41.0
48	33.319729359155644	36.0	32.0	40.0	18.0	41.0
49	33.210295293315184	36.0	32.0	40.0	17.0	41.0
50	33.139274787027766	36.0	32.0	40.0	17.0	41.0
51	32.73129437776015	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	4.0
13	13.0
14	28.0
15	118.0
16	317.0
17	747.0
18	1711.0
19	3353.0
20	6013.0
21	9376.0
22	14645.0
23	21857.0
24	32984.0
25	51235.0
26	74060.0
27	82776.0
28	81012.0
29	81050.0
30	86759.0
31	100907.0
32	121067.0
33	151193.0
34	220567.0
35	299970.0
36	264669.0
37	310693.0
38	447939.0
39	704425.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.842759890486747	24.241480637367644	27.84959405766293	19.06616541448268
2	34.36527301649198	25.16949756787948	28.563045523465906	11.902183892162634
3	26.140011143143973	24.416862113373604	37.538387373795686	11.904739369686736
4	23.204903235739334	26.53405946753944	36.83427444314252	13.426762853578712
5	21.131432941430347	30.565940469362346	35.61401815083122	12.688608438376079
6	20.14571269744219	38.62414983048666	31.816263058963383	9.41387441310777
7	84.2634956030012	2.4752481494872325	12.188681316809175	1.0725749307023904
8	85.0460080601654	1.725073525189752	11.999512881815159	1.2294055328296833
9	80.26199638952039	3.382758161595779	14.503943953645532	1.8513014952382933
10	31.538662481993345	21.383415644948894	35.837575110532285	11.240346762525467
11	29.40136834779229	25.027652790739204	28.333368037349093	17.237610824119418
12	26.588734066913126	23.550618330913515	31.564816690233847	18.295830911939508
13	21.996383210575765	27.56287578927974	32.611079667169555	17.829661332974936
14	18.288669265050974	30.098824417240706	33.48880732393549	18.123698993772837
15	18.319366544445433	26.967228682427336	38.41245533435426	16.300949438772967
16	20.537521035365916	25.306420684602966	37.49705804593676	16.65900023409436
17	20.56282341776505	25.893707278567874	32.73046148138193	20.81300782228515
18	21.348269941716183	26.151431919239336	34.86643055316308	17.633867585881397
19	23.05012332545237	27.561392981333654	31.36851815745654	18.01996553575744
20	24.250724525201743	27.14339888606419	31.96725707662205	16.638619512112015
21	21.888359074248296	27.830790790942377	33.301500286150386	16.97934984865894
22	21.880881936307404	24.676195758412096	33.50565454613142	19.937267759149083
23	19.73809825779531	27.92029560249642	33.18341198525269	19.158194154455586
24	19.158446547297473	26.787777371845955	36.73511560538631	17.318660475470253
25	19.05701617396429	28.10873840807001	34.653852682084086	18.180392735881618
26	19.233407221337796	30.966298614804987	31.732279340825613	18.068014823031604
27	18.088206250382534	30.419615719278585	33.58834475095452	17.90383327938436
28	17.24991497516139	29.54286608477497	35.28063875580425	17.926580184259393
29	18.191434922714155	28.08778980219342	35.103585177220786	18.61719009787163
30	18.499953622815305	29.42985718982024	34.045743678663534	18.02444550870093
31	21.176138023549516	28.613240149896107	32.086796636360596	18.12382519019378
32	21.921611831166857	28.573015040720428	31.615232160400698	17.890140967712014
33	21.141623302421518	28.864371027573288	31.102527651213286	18.89147801879191
34	18.727643515302262	29.28766537252237	32.08704902920248	19.897642082972887
35	19.465924126925838	29.432696609291465	31.787584922304017	19.313794341478683
36	21.94060439251882	29.64467504737098	29.641330842215986	18.77338971789421
37	19.54233605980701	31.245918334510115	30.151953110457836	19.059792495225043
38	20.12037876593782	31.347064765896178	29.274793337586154	19.257763130579853
39	19.969605592015803	30.488834456165986	29.318299553706357	20.22326039811185
40	21.297002645707966	29.38354310333405	29.681713696917843	19.637740554040146
41	18.595263469732735	29.0632250378747	30.012695359946896	22.328816132445667
42	19.976167805904858	29.843781450514285	29.015869830915726	21.164180912665138
43	19.73137829838008	29.63211850348712	29.084362938382704	21.552140259750093
44	19.87568390572875	30.70690187155602	28.970218275639485	20.447195947075745
45	19.285968030660683	32.00584163232546	27.92449163349278	20.78369870352107
46	20.33664788232941	31.529544790580193	28.602134864853095	19.5316724622373
47	20.01017774134908	30.14293006636039	29.240183969142453	20.606708223148082
48	20.319043481607817	29.740047992498887	30.219121155504908	19.721787370388387
49	20.111828958418908	28.44811213309179	30.755077355251125	20.684981553238167
50	19.1440286062047	30.384943252624414	29.864666958180397	20.60636118299049
51	18.35353422541583	31.05529864067525	29.41957218151336	21.171594952395555
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1810.0
1	2515.5
2	3221.0
3	44185.5
4	85150.0
5	58048.5
6	30947.0
7	31394.5
8	31842.0
9	34331.0
10	36820.0
11	39021.5
12	41223.0
13	41155.0
14	41087.0
15	38760.0
16	36433.0
17	33475.0
18	30517.0
19	28756.0
20	26995.0
21	26579.0
22	26163.0
23	25738.0
24	25313.0
25	28244.5
26	34348.5
27	37521.0
28	45091.0
29	52661.0
30	60465.0
31	68269.0
32	77101.0
33	85933.0
34	94135.5
35	102338.0
36	110933.0
37	119528.0
38	131004.5
39	142481.0
40	162105.5
41	181730.0
42	198131.0
43	214532.0
44	227422.5
45	240313.0
46	246027.5
47	251742.0
48	247378.0
49	243014.0
50	232484.0
51	221954.0
52	202174.5
53	182395.0
54	165551.5
55	148708.0
56	137722.5
57	126737.0
58	112379.0
59	98021.0
60	87597.0
61	77173.0
62	64740.0
63	52307.0
64	41528.5
65	30750.0
66	23650.5
67	16551.0
68	13450.0
69	10349.0
70	8164.5
71	5980.0
72	5032.0
73	4084.0
74	3360.5
75	2146.0
76	1655.0
77	1242.0
78	829.0
79	640.5
80	452.0
81	317.0
82	182.0
83	122.0
84	62.0
85	46.5
86	31.0
87	27.5
88	24.0
89	19.0
90	14.0
91	9.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3169662.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.40706493802184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.56813738106162	20.215928334380752
2	7.530136354229983	3.8263732668816033
3	2.813710973369486	2.144644122516695
4	1.5271410184726046	1.5520068410340109
5	1.0222201694902167	1.298580711359681
6	0.7376429546051835	1.1244805469236914
7	0.5535024925243922	0.9844011639646926
8	0.42386446656907356	0.861532162163235
9	0.3437029998962377	0.7859235993980963
>10	3.723661169274158	21.912817720740893
>50	1.1361853870790992	20.713418584467057
>100	0.6164443806017742	21.89363080636602
>500	0.0026432865292605303	0.46319937180546183
>1k	8.810955097535099E-4	0.33665422445430104
>5k	0.0	0.0
>10k+	1.258707871076443E-4	1.886408543543826
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58987	1.8609870705456923	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05401206816373481	0.0
2	0.0	0.0	0.0	0.16172071343884617	0.0
3	0.0	0.0	0.0	0.24267571747397673	0.0
4	0.0	0.0	0.0	0.38871652561061715	0.0
5	0.0	0.0	0.0	0.7225060590056606	0.0
6	0.0	0.0	0.0	1.1997178248027707	0.0
7	0.0	0.0	0.0	1.4742265894596964	0.0
8	0.0	0.0	0.0	1.8874567698385507	0.0
9	0.0	0.0	0.0	2.0612607905827183	0.0
10	0.0	0.0	0.0	2.3721772226817874	0.0
11	0.0	0.0	0.0	2.703947613341738	0.0
12	0.0	0.0	0.0	2.933498903037611	0.0
13	0.0	0.0	0.0	3.0500728468839897	0.0
14	0.0	0.0	0.0	3.1074291202027218	0.0
15	0.0	0.0	0.0	3.1807176916655466	0.0
16	0.0	0.0	0.0	3.352029333096084	0.0
17	0.0	0.0	0.0	3.5536912137634866	0.0
18	0.0	0.0	0.0	3.7763017003074775	0.0
19	0.0	0.0	0.0	3.904958951459178	0.0
20	0.0	0.0	0.0	4.037496742554884	0.0
21	0.0	0.0	0.0	4.227327708758852	0.0
22	0.0	0.0	0.0	4.445931458937893	0.0
23	0.0	0.0	0.0	4.68920660941135	0.0
24	0.0	0.0	0.0	4.871749732305842	0.0
25	0.0	0.0	0.0	5.050286118835384	0.0
26	0.0	0.0	0.0	5.21352118932555	0.0
27	0.0	0.0	0.0	5.374579371554443	0.0
28	0.0	0.0	0.0	5.542546807829983	0.0
29	0.0	0.0	0.0	5.730894966087867	0.0
30	0.0	0.0	0.0	5.944955645112949	0.0
31	0.0	0.0	0.0	6.139739820838941	0.0
32	0.0	0.0	0.0	6.3349341349329995	0.0
33	0.0	0.0	0.0	6.52754142239772	0.0
34	0.0	0.0	0.0	6.722956580228428	0.0
35	0.0	0.0	0.0	6.935502902202191	0.0
36	0.0	0.0	0.0	7.144925862757606	0.0
37	0.0	0.0	0.0	7.3564626133638225	0.0
38	0.0	0.0	0.0	7.584373349587432	0.0
39	0.0	0.0	0.0	7.857683248245396	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	90	0.0	45.0	1
CGTATCG	20	7.035166E-4	45.0	41
ATAGCGC	25	3.8923645E-5	45.0	15
CGCCGAT	20	7.035166E-4	45.0	15
CGTTTTT	32130	0.0	44.145657	1
TACGGGT	930	0.0	41.12903	4
TAGGGTA	3770	0.0	39.688328	5
ACGGGTA	750	0.0	39.3	5
GTTAGCG	150	0.0	39.0	1
TATAGCG	225	0.0	39.0	1
AGGGTAC	2545	0.0	38.634575	6
GTTTTTT	37135	0.0	38.601727	2
TAGGGCG	930	0.0	38.46774	5
CGTTAGG	330	0.0	38.18182	2
CGTTCGA	65	9.094947E-12	38.076927	14
AGGGCGA	2310	0.0	37.987015	6
TTAGGGT	3500	0.0	37.92857	4
GGGCGAT	4410	0.0	37.806126	7
GCGATGC	310	0.0	37.741936	9
ATAGGGT	3495	0.0	37.53219	4
>>END_MODULE