FastQCFastQC Report
Thu 26 May 2016
SRR1547418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547418_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1546513
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT536523.469223989711047No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTT21060.13617732279004444No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18560.12001192359844372No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT16470.10649764987426552No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT1300.045.00000428
CGTTGCG207.034044E-445.01
CGCGATG207.034044E-445.01
AACGGCT207.034044E-445.044
TACGTAC207.034044E-445.024
CGTTTTT206400.044.4985471
TATAGCG1100.042.9545441
CGTTAGG2250.042.02
GGTACGA650.041.5384648
TTGCGAG1450.040.344831
CGGTCTA1450.040.3448331
CGGGTTC900.040.06
GTTTTTT232700.039.7786832
CGTGCGG850.039.7058832
TAATGCG850.039.7058831
TACGGGA5050.039.653464
CGGGTAT1650.039.5454526
TCGCGAG403.459263E-739.3751
ACGGGTC1150.039.1304325
TAGGGCG5300.039.0566025
Produced by FastQC (version 0.11.5)