##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547418_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1546513
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.398341947335716	31.0	31.0	33.0	30.0	34.0
2	31.78621000922721	31.0	31.0	34.0	30.0	34.0
3	31.67956428429635	31.0	31.0	34.0	30.0	34.0
4	35.56693283535282	37.0	35.0	37.0	33.0	37.0
5	35.52302179160473	37.0	35.0	37.0	33.0	37.0
6	35.59032998752678	37.0	35.0	37.0	33.0	37.0
7	36.015616422235055	37.0	35.0	37.0	35.0	37.0
8	36.026845555129505	37.0	35.0	37.0	35.0	37.0
9	37.77654439374257	39.0	38.0	39.0	35.0	39.0
10	37.07460978342891	39.0	37.0	39.0	33.0	39.0
11	36.79010328396851	39.0	37.0	39.0	32.0	39.0
12	36.61497381528639	39.0	35.0	39.0	32.0	39.0
13	36.52647278102415	39.0	35.0	39.0	32.0	39.0
14	37.710820406941295	40.0	36.0	41.0	32.0	41.0
15	37.815085291879214	40.0	36.0	41.0	33.0	41.0
16	37.86147093493556	40.0	36.0	41.0	33.0	41.0
17	37.7944588891267	40.0	36.0	41.0	33.0	41.0
18	37.55404707234921	39.0	36.0	41.0	33.0	41.0
19	37.35175132701762	38.0	36.0	41.0	33.0	41.0
20	37.039675709159894	38.0	35.0	41.0	32.0	41.0
21	36.93239759381266	38.0	35.0	41.0	32.0	41.0
22	36.85140053785516	38.0	35.0	41.0	32.0	41.0
23	36.760993279720246	38.0	35.0	40.0	32.0	41.0
24	36.59301409040855	38.0	35.0	40.0	31.0	41.0
25	36.49100201550197	38.0	35.0	40.0	31.0	41.0
26	36.32053335471477	38.0	35.0	40.0	31.0	41.0
27	36.20975381390263	38.0	35.0	40.0	31.0	41.0
28	36.16050172226163	38.0	35.0	40.0	31.0	41.0
29	36.110743330317945	38.0	35.0	40.0	30.0	41.0
30	35.97956887526972	38.0	35.0	40.0	30.0	41.0
31	35.77355573474002	38.0	35.0	40.0	30.0	41.0
32	35.48576701262776	38.0	34.0	40.0	28.0	41.0
33	35.02143919902387	37.0	34.0	40.0	25.0	41.0
34	34.52573175912521	37.0	34.0	40.0	22.0	41.0
35	34.14571038200131	37.0	33.0	40.0	20.0	41.0
36	33.89037596192208	37.0	33.0	40.0	18.0	41.0
37	33.75485172125938	37.0	33.0	40.0	18.0	41.0
38	33.64477440538812	37.0	33.0	40.0	18.0	41.0
39	33.58317582846054	37.0	33.0	40.0	17.0	41.0
40	33.4407735337498	37.0	33.0	40.0	16.0	41.0
41	33.3435289583728	36.0	33.0	40.0	15.0	41.0
42	33.25488308213381	36.0	33.0	40.0	15.0	41.0
43	33.18921599753769	36.0	33.0	40.0	15.0	41.0
44	33.0585885795981	36.0	33.0	40.0	15.0	41.0
45	32.98646050825308	36.0	32.0	40.0	15.0	41.0
46	32.90011981793881	36.0	32.0	40.0	15.0	41.0
47	32.777351370470214	35.0	32.0	40.0	14.0	41.0
48	32.70897431835361	35.0	32.0	40.0	13.0	41.0
49	32.59582040370821	35.0	31.0	40.0	12.0	41.0
50	32.52884780147338	35.0	31.0	40.0	12.0	41.0
51	32.15532556144048	35.0	31.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	2.0
11	1.0
12	3.0
13	8.0
14	15.0
15	43.0
16	153.0
17	357.0
18	844.0
19	1745.0
20	2956.0
21	4823.0
22	7521.0
23	11702.0
24	17812.0
25	28838.0
26	41695.0
27	44969.0
28	41233.0
29	39470.0
30	41627.0
31	47463.0
32	56782.0
33	71041.0
34	104023.0
35	151656.0
36	149331.0
37	151347.0
38	202824.0
39	326132.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.465651436489704	22.847657924634323	26.416396111768865	22.27029452710711
2	38.117817309004195	23.59960763343082	26.62848614916267	11.654088908402322
3	24.881200481340926	23.068477277591587	40.26975524939008	11.780566991677405
4	21.65497477227802	25.81678912495401	39.49278150264498	13.035454600122986
5	20.269535399961075	29.48316632320582	38.33811936918733	11.909178907645781
6	18.894506544723516	37.01895813355594	34.969638147238335	9.116897174482206
7	80.43288352571236	2.7889839917284887	15.688390592254963	1.0897418903041876
8	80.9395717979739	2.462248943267855	15.43588705688216	1.1622922018760915
9	76.84144911811282	4.8979219702647185	16.472153806660533	1.788475104961937
10	34.10297876577823	30.67494421320739	25.23593400120141	9.98614301981296
11	24.84505464874851	26.168354226572944	33.61614160372399	15.37044952095456
12	24.817056177348654	23.77005560250706	34.79265935688869	16.620228863255594
13	20.0502679253262	28.446252957459784	35.476649727483704	16.026829389730317
14	16.934936854717677	31.186870074807	34.9858682080267	16.892324862448618
15	16.39113282591223	29.015081024213828	39.54483408804194	15.048952061832004
16	18.28144994578125	27.955406776406015	38.66537171042209	15.09777156739064
17	17.84375559726947	28.607195671811358	34.24859668169618	19.300452049222994
18	18.467287374887892	29.622900033818016	35.90677866917381	16.00303392212028
19	20.751522942257843	31.09311075949572	32.10448279451902	16.050883503727416
20	21.741168680767636	30.109866519065797	33.25009230442939	14.898872495737184
21	20.118162601930926	30.409249712094237	34.51778290903471	14.954804776940122
22	19.871349287073564	28.767621093388808	33.659852843138076	17.701176776399553
23	17.60075731662133	31.852431890323587	33.55296722368322	16.993843569371872
24	17.22772456487595	30.999868736958565	36.68840805088609	15.083998647279396
25	17.459924358864104	32.326336733024554	34.379148445567544	15.8345904625438
26	16.975285691099913	34.423376977755765	32.228568398713755	16.37276893243057
27	16.711207729905926	33.66787088113711	33.66838817391125	15.952533215045719
28	15.760035641472136	32.5602823901254	36.03215750530387	15.647524463098597
29	16.64777470347808	30.356421187536085	35.97771244082655	17.018091668159272
30	17.084693112828667	33.02403536213404	34.381670247841434	15.50960127719586
31	19.498316535328186	32.295945782544344	32.68184619204624	15.523891490081235
32	19.883053036088285	31.600962940499045	32.881779849247955	15.634204174164717
33	19.493984208344838	32.59681619229842	31.69329970068147	16.21589989867528
34	17.66755274608102	32.9534895600619	31.752659046513028	17.626298647344058
35	18.00877199221733	33.19351340725878	31.32097822650052	17.476736374023368
36	20.520293072221186	33.874400021209006	28.838231557057714	16.767075349512094
37	18.153872615361138	35.00727119655638	30.36359862477716	16.47525756330532
38	18.18549213617991	35.49902263996488	28.918282613854522	17.397202610000694
39	18.169520721778607	35.31163333253584	28.849547336491838	17.669298609193714
40	20.037076959585857	33.862049656226624	28.78527370930603	17.31559967488149
41	17.025786398174475	33.00353763595909	30.46175492866856	19.508921037197876
42	18.539449716879197	33.25403666183214	28.452654455539655	19.75385916574901
43	18.54785572445883	33.775015146979044	28.92119238570901	18.755936742853116
44	18.160274113441012	34.672582771693484	28.532188219562332	18.634954895303178
45	17.666259514145693	35.69824501960216	27.30840283916139	19.327092627090753
46	18.81445548792671	35.59491578796945	27.803710670392036	17.786918053711805
47	18.292571740425075	34.172748628689185	29.06021481875678	18.474464812128964
48	18.358332584336505	33.53453866860479	30.240676929324227	17.86645181773448
49	18.371264903689784	33.13214955192746	29.363219061204145	19.13336648317861
50	17.717083529204086	34.554058064820666	29.86835545514328	17.860502950831968
51	17.04259841333374	35.0436110139391	28.617088896116616	19.296701676610542
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1441.0
1	1747.5
2	2054.0
3	34399.0
4	66744.0
5	42702.0
6	18660.0
7	19606.5
8	20553.0
9	24235.5
10	27918.0
11	31508.5
12	35099.0
13	36355.5
14	37612.0
15	35959.5
16	34307.0
17	30931.0
18	27555.0
19	24826.0
20	22097.0
21	20244.5
22	18392.0
23	18020.5
24	17649.0
25	17781.0
26	19309.5
27	20706.0
28	23238.0
29	25770.0
30	28958.0
31	32146.0
32	37078.0
33	42010.0
34	46109.0
35	50208.0
36	53943.0
37	57678.0
38	61783.0
39	65888.0
40	75761.0
41	85634.0
42	91788.5
43	97943.0
44	99946.5
45	101950.0
46	104168.0
47	106386.0
48	105279.0
49	104172.0
50	99108.0
51	94044.0
52	86820.5
53	79597.0
54	71353.0
55	63109.0
56	56959.5
57	50810.0
58	45858.0
59	40906.0
60	34850.0
61	28794.0
62	24855.0
63	20916.0
64	16930.0
65	12944.0
66	10021.0
67	7098.0
68	5543.5
69	3989.0
70	3061.0
71	2133.0
72	1714.0
73	1295.0
74	1206.5
75	882.5
76	647.0
77	471.5
78	296.0
79	221.0
80	146.0
81	119.0
82	92.0
83	79.5
84	67.0
85	40.5
86	14.0
87	11.0
88	8.0
89	5.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1546513.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.950779218361724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.72372677842198	22.679846223320617
2	7.814094011706853	4.680764090723087
3	3.8162679665742245	3.4290059791491245
4	2.2838492773690304	2.736122618979792
5	1.5076325333779477	2.2577384574811137
6	1.0603633272717292	1.9055224743817802
7	0.8087949177488705	1.6956826610100613
8	0.6038842260385977	1.4469442502026628
9	0.4814326370011163	1.2977354357400703
>10	4.964472659691748	33.423511615675636
>50	0.8658342209683454	17.131411788734827
>100	0.06594742423331183	2.7715052599788255
>500	0.0023941303269866066	0.5136110149258266
>1k	0.0010882410577211848	0.5331672155195248
>5k	0.0	0.0
>10k+	2.1764821154423696E-4	3.4974309141769835
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53652	3.469223989711047	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTT	2106	0.13617732279004444	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1856	0.12001192359844372	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	1647	0.10649764987426552	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.466159676640286E-5	0.0	0.0	0.04435785538175237	0.0
2	6.466159676640286E-5	0.0	0.0	0.14025100338632782	0.0
3	6.466159676640286E-5	0.0	0.0	0.234010318697612	0.0
4	6.466159676640286E-5	0.0	0.0	0.3386327822656518	0.0
5	6.466159676640286E-5	0.0	0.0	0.5931408271382135	0.0
6	6.466159676640286E-5	0.0	0.0	1.030253221279097	0.0
7	6.466159676640286E-5	0.0	0.0	1.3102379352776212	0.0
8	6.466159676640286E-5	0.0	0.0	1.6894135387158078	0.0
9	6.466159676640286E-5	0.0	0.0	1.8855968233050742	0.0
10	6.466159676640286E-5	0.0	0.0	2.0947124272476207	0.0
11	6.466159676640286E-5	0.0	0.0	2.326847559639007	0.0
12	6.466159676640286E-5	0.0	0.0	2.474340661863172	0.0
13	6.466159676640286E-5	0.0	0.0	2.5609225399333857	0.0
14	6.466159676640286E-5	0.0	0.0	2.6094187375081876	0.0
15	6.466159676640286E-5	0.0	0.0	2.6537119312931736	0.0
16	6.466159676640286E-5	0.0	0.0	2.7271675052198074	0.0
17	6.466159676640286E-5	0.0	0.0	2.8180170486766034	0.0
18	6.466159676640286E-5	0.0	0.0	2.9215402650996145	0.0
19	1.2932319353280573E-4	0.0	0.0	2.993961253477986	0.0
20	1.2932319353280573E-4	0.0	0.0	3.067416827404619	0.0
21	1.2932319353280573E-4	0.0	0.0	3.1596242643935097	0.0
22	1.2932319353280573E-4	0.0	0.0	3.2634061272035866	0.0
23	1.2932319353280573E-4	0.0	0.0	3.385810529882387	0.0
24	1.2932319353280573E-4	0.0	0.0	3.4805397691451674	0.0
25	1.2932319353280573E-4	0.0	0.0	3.572294574956693	0.0
26	1.2932319353280573E-4	0.0	0.0	3.657518559494812	0.0
27	1.2932319353280573E-4	0.0	0.0	3.754704939434715	0.0
28	1.2932319353280573E-4	0.0	0.0	3.8503394410522254	0.0
29	1.2932319353280573E-4	0.0	0.0	3.962527311441934	0.0
30	1.2932319353280573E-4	0.0	0.0	4.089716672281448	0.0
31	1.2932319353280573E-4	0.0	0.0	4.201581234687326	0.0
32	1.2932319353280573E-4	0.0	0.0	4.315191660205896	0.0
33	1.2932319353280573E-4	0.0	0.0	4.431647195982187	0.0
34	1.2932319353280573E-4	0.0	0.0	4.555344830596316	0.0
35	1.2932319353280573E-4	0.0	0.0	4.695595833982644	0.0
36	1.2932319353280573E-4	0.0	0.0	4.828281430547302	0.0
37	1.2932319353280573E-4	0.0	0.0	4.964523414934113	0.0
38	1.2932319353280573E-4	0.0	0.0	5.110917270013249	0.0
39	1.2932319353280573E-4	0.0	0.0	5.299341162990547	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	130	0.0	45.000004	28
CGTTGCG	20	7.034044E-4	45.0	1
CGCGATG	20	7.034044E-4	45.0	1
AACGGCT	20	7.034044E-4	45.0	44
TACGTAC	20	7.034044E-4	45.0	24
CGTTTTT	20640	0.0	44.498547	1
TATAGCG	110	0.0	42.954544	1
CGTTAGG	225	0.0	42.0	2
GGTACGA	65	0.0	41.538464	8
TTGCGAG	145	0.0	40.34483	1
CGGTCTA	145	0.0	40.34483	31
CGGGTTC	90	0.0	40.0	6
GTTTTTT	23270	0.0	39.778683	2
CGTGCGG	85	0.0	39.705883	2
TAATGCG	85	0.0	39.705883	1
TACGGGA	505	0.0	39.65346	4
CGGGTAT	165	0.0	39.545452	6
TCGCGAG	40	3.459263E-7	39.375	1
ACGGGTC	115	0.0	39.130432	5
TAGGGCG	530	0.0	39.056602	5
>>END_MODULE