FastQCFastQC Report
Thu 26 May 2016
SRR1547417_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547417_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2418652
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270721.1193011644502806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGACG302.1665E-645.0000041
CTATACG406.8212103E-945.01
TATGCGA207.0348347E-445.02
TCGACGA253.8920884E-545.040
CGTTTTT120000.043.01251
CGTTAGG4100.042.804882
CGACGGT1550.042.0967728
TAGTTCG603.6379788E-1241.2500041
GGCACCG10700.040.3738338
ACGGGTC2600.039.8076935
GACCGAT11200.039.5758939
ATGGGCG7050.039.255325
GGGCGAT38900.039.1580967
TATTGCG1850.038.918921
ACGTAAG1100.038.8636361
AGGGCGA18150.038.8016556
GCACCGA11000.038.259
TAGGGCG8300.038.2228935
CTCGTCC7300.038.21917737
TCGATTG659.094947E-1238.0769271
Produced by FastQC (version 0.11.5)