##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547417_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2418652
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229940892695602	31.0	31.0	33.0	30.0	34.0
2	31.631493493069694	31.0	31.0	34.0	30.0	34.0
3	31.72752549767391	31.0	31.0	34.0	30.0	34.0
4	35.520175701175695	37.0	35.0	37.0	33.0	37.0
5	35.40811658725604	37.0	35.0	37.0	33.0	37.0
6	35.44328245650883	37.0	35.0	37.0	33.0	37.0
7	35.88645824202903	37.0	35.0	37.0	35.0	37.0
8	35.86841844134667	37.0	35.0	37.0	35.0	37.0
9	37.55768791872497	39.0	37.0	39.0	35.0	39.0
10	36.80094159887408	39.0	37.0	39.0	32.0	39.0
11	36.54506229089592	39.0	35.0	39.0	32.0	39.0
12	36.129078098048005	38.0	35.0	39.0	31.0	39.0
13	35.94380258094178	38.0	35.0	39.0	31.0	39.0
14	37.03366296598271	39.0	35.0	41.0	31.0	41.0
15	37.19201811587612	39.0	35.0	41.0	32.0	41.0
16	37.270743372754744	39.0	35.0	41.0	32.0	41.0
17	37.184990234229645	39.0	35.0	41.0	32.0	41.0
18	37.08356100836334	39.0	35.0	41.0	32.0	41.0
19	37.013837046420896	38.0	35.0	41.0	31.0	41.0
20	36.853896302568536	38.0	35.0	41.0	31.0	41.0
21	36.73572303911435	38.0	35.0	40.0	31.0	41.0
22	36.63473166044557	38.0	35.0	40.0	31.0	41.0
23	36.54887019711806	38.0	35.0	40.0	31.0	41.0
24	36.404517475023276	38.0	35.0	40.0	31.0	41.0
25	36.324910735401374	38.0	35.0	40.0	30.0	41.0
26	36.16475954374585	38.0	35.0	40.0	30.0	41.0
27	36.06413365792185	38.0	35.0	40.0	30.0	41.0
28	36.12238552714487	38.0	35.0	40.0	30.0	41.0
29	36.142920105910235	38.0	35.0	40.0	30.0	41.0
30	36.07996603066501	38.0	35.0	40.0	30.0	41.0
31	35.98257831221689	38.0	35.0	40.0	30.0	41.0
32	35.85702531823512	38.0	35.0	40.0	29.0	41.0
33	35.61390022210719	38.0	34.0	40.0	28.0	41.0
34	35.38240143683341	38.0	34.0	40.0	27.0	41.0
35	35.22544334612834	38.0	34.0	40.0	26.0	41.0
36	35.04712501012961	38.0	34.0	40.0	25.0	41.0
37	34.950580736707884	38.0	34.0	40.0	25.0	41.0
38	34.897043890563836	38.0	34.0	40.0	24.0	41.0
39	34.872168050633164	38.0	34.0	40.0	24.0	41.0
40	34.705757587284154	37.0	34.0	40.0	24.0	41.0
41	34.640319897198935	37.0	34.0	40.0	24.0	41.0
42	34.56797670768676	37.0	33.0	40.0	23.0	41.0
43	34.522961550483494	37.0	33.0	40.0	23.0	41.0
44	34.38421112255918	37.0	33.0	40.0	23.0	41.0
45	34.29095545783354	37.0	33.0	40.0	23.0	41.0
46	34.19819841796174	37.0	33.0	40.0	23.0	41.0
47	34.06896444796523	36.0	33.0	40.0	23.0	41.0
48	34.00172658158346	36.0	33.0	40.0	23.0	41.0
49	33.87543681356392	36.0	33.0	40.0	23.0	41.0
50	33.79465710652049	36.0	33.0	40.0	22.0	41.0
51	33.34375842411393	35.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	2.0
12	5.0
13	23.0
14	37.0
15	75.0
16	212.0
17	567.0
18	1258.0
19	2461.0
20	4256.0
21	6584.0
22	9998.0
23	14685.0
24	20908.0
25	30207.0
26	40842.0
27	48199.0
28	53268.0
29	59311.0
30	68465.0
31	82177.0
32	98900.0
33	125691.0
34	190025.0
35	257540.0
36	201486.0
37	235859.0
38	336548.0
39	528879.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.4688710901775	24.697765532205544	28.246725862174465	14.586637515442485
2	31.55757835356223	25.76232546062848	29.65726363280042	13.022832553008868
3	27.956233472198566	25.254521940320473	33.28337437547857	13.505870212002389
4	24.305646285616948	28.494095057908293	32.64946755465441	14.550791101820353
5	22.362993932157252	32.45402811152658	31.605290881036215	13.577687075279949
6	21.026340292030437	41.00705682338757	28.314408191008877	9.652194693573115
7	87.5803960222471	3.225598391169957	7.778134266525321	1.415871320057619
8	87.9662307764821	2.797963493714681	7.44869456209492	1.7871111677082936
9	82.76494510165166	5.712562204070697	9.102549684700403	2.419943009577236
10	38.933463764113235	32.79624352738633	16.410504694350404	11.85978801415003
11	33.12369865528402	25.331176208896526	25.53558759176599	16.009537544053465
12	31.494816120715175	22.62280807656496	28.53552309302868	17.34685270969118
13	22.966884032924124	29.453017631308683	29.820536397960517	17.75956193780668
14	18.199352366524824	32.495042693202656	30.68291759211329	18.62268734815922
15	17.73764890525797	28.046779776503605	37.595404382275746	16.620166935962676
16	20.3857768707528	25.07181686327756	37.452597562609256	17.089808703360383
17	19.423298597731296	26.602586895510395	29.861137526192277	24.112976980566035
18	20.8441313591207	27.90740462042493	31.814787741270756	19.43367627918361
19	24.56194607574798	29.71775187170374	27.40811824106982	18.312183811478462
20	26.91267697874684	27.216193152218676	28.251191159373075	17.619938709661415
21	23.040189328601222	29.80867028410867	29.348041801797038	17.80309858549308
22	22.719845599945753	26.655054137593996	28.94211321016831	21.68298705229194
23	21.27916707322922	30.194670419721398	28.311472671554238	20.214689835495143
24	20.723278917347347	27.69013483543726	33.532934874467266	18.053651372748124
25	19.986422188888685	29.315627051762718	30.49574721787177	20.202203541476823
26	19.2234352027493	33.20771239516888	28.326150268827426	19.24270213325439
27	19.885787620542352	33.05986144348174	28.433689509693828	18.62066142628208
28	18.151226385606527	31.291314335423202	32.570539292134626	17.98691998683564
29	19.034280251975066	27.932377208461574	32.73306784109496	20.300274698468403
30	20.063365874875757	30.49665681544927	30.41462765209712	19.02534965757786
31	23.488827661027713	28.924293366718324	27.78923962603963	19.79763934621434
32	24.414467232160725	28.713349419428674	28.572237758883873	18.29994558952673
33	23.465550232112765	29.458723288840226	27.97641000028115	19.099316478765857
34	20.25330638719419	29.249433155327843	29.48018152260019	21.017078934877777
35	21.75298472041451	27.745000107497898	30.16928437824044	20.33273079384715
36	23.956484851892707	29.307440673565278	27.67516782075305	19.06090665378897
37	20.106406378428975	32.149767721854985	28.65831876599031	19.08550713372573
38	19.946234514101242	31.80515427601821	28.235562619177955	20.01304859070259
39	20.679618233627657	30.697305771975465	28.615236917092663	20.00783907730422
40	22.673332087460288	28.525889627776134	27.994850023897612	20.80592826086597
41	18.018466484636896	29.376735470832514	29.43461895303665	23.17017909149394
42	19.984809720455857	29.207674357452003	27.731232107802196	23.076283814289944
43	20.96411554866099	28.88257591418691	28.087918394212974	22.065390142939126
44	19.864205350749096	30.423930354594216	28.165523605710952	21.54634068894574
45	18.91896808635554	32.818859430790376	26.23151242923744	22.030660053616643
46	20.510846537658168	32.45427618359317	26.2874526802533	20.74742459849536
47	20.703598533397944	29.19440250188948	28.975561593813413	21.12643737089916
48	20.90201484132484	27.38694942472088	30.81811686840438	20.892918865549902
49	19.84344998784447	28.060754502921466	30.11028457173665	21.985510937497416
50	18.905324122693138	30.415082450885865	29.389097728817536	21.290495697603458
51	18.456437718200057	30.283810982315767	27.715975675706968	23.54377562377721
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1920.0
1	2868.5
2	3817.0
3	21550.0
4	39283.0
5	26475.0
6	13667.0
7	14011.0
8	14355.0
9	16098.5
10	17842.0
11	19379.0
12	20916.0
13	21135.0
14	21354.0
15	19884.0
16	18414.0
17	17695.0
18	16976.0
19	16084.0
20	15192.0
21	14580.0
22	13968.0
23	16127.5
24	18287.0
25	19912.5
26	26012.5
27	30487.0
28	35633.5
29	40780.0
30	46349.5
31	51919.0
32	58727.0
33	65535.0
34	75471.5
35	85408.0
36	88335.5
37	91263.0
38	100057.5
39	108852.0
40	127639.0
41	146426.0
42	159463.0
43	172500.0
44	181944.5
45	191389.0
46	192946.5
47	194504.0
48	190825.0
49	187146.0
50	176491.0
51	165836.0
52	151211.0
53	136586.0
54	126082.0
55	115578.0
56	104389.0
57	93200.0
58	91958.5
59	90717.0
60	82906.5
61	75096.0
62	63200.0
63	51304.0
64	42617.5
65	33931.0
66	26955.0
67	19979.0
68	15963.0
69	11947.0
70	9939.0
71	7931.0
72	6819.0
73	5707.0
74	4579.5
75	2700.5
76	1949.0
77	1406.5
78	864.0
79	606.5
80	349.0
81	281.5
82	214.0
83	169.5
84	125.0
85	112.5
86	100.0
87	64.0
88	28.0
89	19.5
90	11.0
91	9.0
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2418652.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.18744605929052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.57384676679588	18.451142796353288
2	8.141107127936046	3.775429647838452
3	2.9379290951366817	2.04369217258506
4	1.5637015783650712	1.4503298400467013
5	0.9391291924932679	1.0888003746821355
6	0.6525691964940337	0.9078847826195977
7	0.47030926335691786	0.7633689472691233
8	0.36646127344701546	0.6797840808697267
9	0.31519763119379335	0.6577765264190006
>10	3.0461533784899246	16.085368609712756
>50	1.0020494921700425	16.800631902228126
>100	0.9857916619217804	35.079584641389964
>500	0.003956110262267496	0.5878776289049306
>1k	0.0016184087436548846	0.49952294448408174
>5k	0.0	0.0
>10k+	1.798231937394316E-4	1.1288051045970835
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27072	1.1193011644502806	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.269068886305264E-5	0.0	0.0	0.06540833489067464	0.0
2	8.269068886305264E-5	0.0	0.0	0.22624172472931203	0.0
3	8.269068886305264E-5	0.0	0.0	0.31645726627890247	0.0
4	8.269068886305264E-5	0.0	0.0	0.5038343672425798	0.0
5	8.269068886305264E-5	0.0	0.0	0.8853278603122731	0.0
6	8.269068886305264E-5	0.0	0.0	1.3777509124917515	0.0
7	8.269068886305264E-5	0.0	0.0	1.6376064022438945	0.0
8	8.269068886305264E-5	0.0	0.0	2.1718709429880776	0.0
9	8.269068886305264E-5	0.0	0.0	2.366400788538409	0.0
10	8.269068886305264E-5	0.0	0.0	2.7306946183245873	0.0
11	8.269068886305264E-5	0.0	0.0	3.1945480375018813	0.0
12	8.269068886305264E-5	0.0	0.0	3.5302722342858748	0.0
13	8.269068886305264E-5	0.0	0.0	3.6866403269259074	0.0
14	8.269068886305264E-5	0.0	0.0	3.748823724950923	0.0
15	8.269068886305264E-5	0.0	0.0	3.8325893927691954	0.0
16	8.269068886305264E-5	0.0	0.0	4.034768127039359	0.0
17	8.269068886305264E-5	0.0	0.0	4.286519929282923	0.0
18	8.269068886305264E-5	0.0	0.0	4.566593292462082	0.0
19	8.269068886305264E-5	0.0	0.0	4.739003378741547	0.0
20	8.269068886305264E-5	0.0	0.0	4.9061212609337765	0.0
21	8.269068886305264E-5	0.0	0.0	5.1445598622703885	0.0
22	8.269068886305264E-5	0.0	0.0	5.407392216821601	0.0
23	8.269068886305264E-5	0.0	0.0	5.714050636470232	0.0
24	8.269068886305264E-5	0.0	0.0	5.932147328346534	0.0
25	8.269068886305264E-5	0.0	0.0	6.1296953840403665	0.0
26	8.269068886305264E-5	0.0	0.0	6.318684953436873	0.0
27	8.269068886305264E-5	0.0	0.0	6.502754426846028	0.0
28	8.269068886305264E-5	0.0	0.0	6.711258998814215	0.0
29	8.269068886305264E-5	0.0	0.0	6.922781780925904	0.0
30	8.269068886305264E-5	0.0	0.0	7.163783793617271	0.0
31	8.269068886305264E-5	0.0	0.0	7.392712965734632	0.0
32	8.269068886305264E-5	0.0	0.0	7.602499243380197	0.0
33	8.269068886305264E-5	0.0	0.0	7.818776740101511	0.0
34	8.269068886305264E-5	0.0	0.0	8.02868705378037	0.0
35	8.269068886305264E-5	0.0	0.0	8.262536321885083	0.0
36	8.269068886305264E-5	0.0	0.0	8.483609878560454	0.0
37	1.2403603329457896E-4	0.0	0.0	8.720063903364354	0.0
38	1.2403603329457896E-4	0.0	0.0	8.969293639597595	0.0
39	1.2403603329457896E-4	0.0	0.0	9.221541585974336	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGACG	30	2.1665E-6	45.000004	1
CTATACG	40	6.8212103E-9	45.0	1
TATGCGA	20	7.0348347E-4	45.0	2
TCGACGA	25	3.8920884E-5	45.0	40
CGTTTTT	12000	0.0	43.0125	1
CGTTAGG	410	0.0	42.80488	2
CGACGGT	155	0.0	42.09677	28
TAGTTCG	60	3.6379788E-12	41.250004	1
GGCACCG	1070	0.0	40.373833	8
ACGGGTC	260	0.0	39.807693	5
GACCGAT	1120	0.0	39.575893	9
ATGGGCG	705	0.0	39.25532	5
GGGCGAT	3890	0.0	39.158096	7
TATTGCG	185	0.0	38.91892	1
ACGTAAG	110	0.0	38.863636	1
AGGGCGA	1815	0.0	38.801655	6
GCACCGA	1100	0.0	38.25	9
TAGGGCG	830	0.0	38.222893	5
CTCGTCC	730	0.0	38.219177	37
TCGATTG	65	9.094947E-12	38.076927	1
>>END_MODULE