FastQCFastQC Report
Thu 26 May 2016
SRR1547416_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547416_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3803032
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292980.7703853136129278No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC253.8925114E-545.044
TTACGCG1100.042.9545441
CGTTTTT149100.042.7515071
TCGTCCC14850.040.6060638
AGGGCGA33950.040.4270976
CTCGTCC15150.039.65346537
GGGCGAT62300.039.474327
CGTAAGG6650.039.248122
GGCGATA11750.038.872348
CCGCGAA356.252094E-638.5714315
TAATCGT2700.038.33333621
TACGGGT7650.038.2352944
AGGGTAC22500.038.26
CGTTAGG6550.038.1297722
TAGTGCG2900.038.0172421
TATACGG3850.037.9870152
CGCATCG900.037.521
GGCGATT18550.037.4797868
GTAGGGA42300.037.393624
TAGGGCA25100.037.2908365