##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547416_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3803032
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259717246660035	31.0	31.0	33.0	30.0	34.0
2	31.67898797590975	31.0	31.0	34.0	30.0	34.0
3	31.82267385601804	31.0	31.0	34.0	30.0	34.0
4	35.60694440646305	37.0	35.0	37.0	33.0	37.0
5	35.483827640682485	37.0	35.0	37.0	33.0	37.0
6	35.521718197480325	37.0	35.0	37.0	33.0	37.0
7	35.91835724758561	37.0	35.0	37.0	35.0	37.0
8	35.896386356990945	37.0	35.0	37.0	35.0	37.0
9	37.623975291293895	39.0	37.0	39.0	35.0	39.0
10	36.761314656305814	39.0	37.0	39.0	32.0	39.0
11	36.57550975116696	39.0	35.0	39.0	32.0	39.0
12	36.217657384949696	38.0	35.0	39.0	32.0	39.0
13	36.064368903548534	38.0	35.0	39.0	31.0	39.0
14	37.13861650388427	39.0	35.0	41.0	31.0	41.0
15	37.250406517746896	39.0	35.0	41.0	32.0	41.0
16	37.298848392545736	39.0	35.0	41.0	32.0	41.0
17	37.20584470496173	39.0	35.0	41.0	32.0	41.0
18	37.08324410628151	39.0	35.0	41.0	31.0	41.0
19	37.05434479646766	39.0	35.0	41.0	31.0	41.0
20	36.92694513219978	39.0	35.0	41.0	31.0	41.0
21	36.8102937340522	38.0	35.0	41.0	31.0	41.0
22	36.73323495568799	38.0	35.0	41.0	31.0	41.0
23	36.655552990350856	38.0	35.0	40.0	31.0	41.0
24	36.515147650611404	38.0	35.0	40.0	31.0	41.0
25	36.44454135542378	38.0	35.0	40.0	30.0	41.0
26	36.312189326831856	38.0	35.0	40.0	30.0	41.0
27	36.22528524608786	38.0	35.0	40.0	30.0	41.0
28	36.292451391416115	38.0	35.0	40.0	30.0	41.0
29	36.32121870128886	38.0	35.0	40.0	30.0	41.0
30	36.26239142873371	38.0	35.0	40.0	30.0	41.0
31	36.16337148885415	38.0	35.0	40.0	30.0	41.0
32	36.06872253507202	38.0	35.0	40.0	30.0	41.0
33	35.868258010976504	38.0	35.0	40.0	29.0	41.0
34	35.65628845615814	38.0	34.0	40.0	28.0	41.0
35	35.505493511492936	38.0	34.0	40.0	27.0	41.0
36	35.38688814608975	38.0	34.0	40.0	27.0	41.0
37	35.302742916704354	38.0	34.0	40.0	26.0	41.0
38	35.22766492630091	38.0	34.0	40.0	26.0	41.0
39	35.180582230178445	38.0	34.0	40.0	26.0	41.0
40	35.04445321522407	38.0	34.0	40.0	25.0	41.0
41	34.97619636121915	38.0	34.0	40.0	25.0	41.0
42	34.900098658123305	38.0	34.0	40.0	25.0	41.0
43	34.84581696919721	37.0	34.0	40.0	25.0	41.0
44	34.71784828526292	37.0	33.0	40.0	24.0	41.0
45	34.6287165083018	37.0	33.0	40.0	24.0	41.0
46	34.546711150471516	37.0	33.0	40.0	24.0	41.0
47	34.3989498379188	37.0	33.0	40.0	23.0	41.0
48	34.35572721975519	37.0	33.0	40.0	23.0	41.0
49	34.22951450316484	36.0	33.0	40.0	24.0	41.0
50	34.15507442482735	36.0	33.0	40.0	24.0	41.0
51	33.714946127195354	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	5.0
12	6.0
13	17.0
14	53.0
15	142.0
16	312.0
17	826.0
18	1833.0
19	3501.0
20	5959.0
21	9469.0
22	14447.0
23	20940.0
24	30180.0
25	42539.0
26	58288.0
27	69751.0
28	78626.0
29	88193.0
30	103510.0
31	124197.0
32	152076.0
33	192044.0
34	293227.0
35	378808.0
36	313739.0
37	388970.0
38	559377.0
39	871739.0
40	253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.34498158311579	24.24799475786688	29.149084204392707	14.257939454624626
2	30.519885186346052	25.63701804244613	30.165930762612568	13.677166008595249
3	27.823405114655884	25.578485797647772	32.857598884258664	13.740510203437678
4	25.304993489405298	27.99282256893973	32.08337452853407	14.6188094131209
5	22.76544083773158	32.26075930994007	31.129372563785946	13.844427288542406
6	21.640154487261743	40.88451004356524	27.364692171930187	10.11064329724283
7	90.206603573149	2.519857839744709	6.128715193561348	1.14482339354494
8	91.00954186028412	1.9816030998424414	5.722881111702452	1.285973928170996
9	86.80234612803679	3.850480353570519	7.54192970240587	1.805243815986823
10	37.78014489491542	27.206134473756727	22.06794473462227	12.945775896705575
11	33.51396990611701	25.402415756690978	23.56932573799011	17.514288599201898
12	31.879773822571046	23.563646059249567	25.49347468020253	19.06310543797686
13	24.828347486952516	27.09396081863103	27.51115425797101	20.566537436445447
14	21.04952048786337	29.051346399399215	29.124919274936417	20.774213837800996
15	21.60697043832395	26.465777832003518	33.5910136964401	18.336238033232434
16	24.400320586311132	24.977097221374944	32.75097343382859	17.871608758485337
17	23.963484924660115	25.588162287353878	28.256296554959302	22.1920562330267
18	24.421566791970196	26.247820160335227	30.15770048740058	19.172912560293998
19	26.013296759007027	28.072548429779186	26.458520464723932	19.455634346489852
20	27.96718513018034	27.633504004173513	26.61018892294359	17.78912194270256
21	26.19975324951249	28.210070280765454	27.8140178678486	17.77615860187345
22	25.047041413272357	24.874389697483483	28.844143304605378	21.23442558463878
23	24.23408480391435	27.26703325136365	28.000553242781024	20.498328701940977
24	23.58717991329024	25.86780758089861	31.954714028175413	18.590298477635738
25	22.28997810168308	27.32756390164479	28.860419791366464	21.52203820530566
26	21.947961521228326	29.75699389329356	27.36634874489618	20.928695840581934
27	21.194115642466326	29.828358004876108	28.356874199323066	20.620652153334497
28	19.063079143167872	29.63664255257384	30.892088207514423	20.408190096743862
29	21.169845533774104	27.299112918324113	30.20760803485219	21.32343351304959
30	22.29915499001849	28.096555590381566	29.53632785629992	20.067961563300017
31	24.264613077144762	27.793402737605156	27.777441788551872	20.164542396698213
32	25.975800361395855	27.168454012482673	27.084941699149525	19.770803926971954
33	24.288252110421368	28.209886217102564	27.412285776191208	20.08957589628486
34	21.695794303071864	27.833213078406914	29.28534390454774	21.18564871397348
35	21.41578088220136	28.45537455377709	29.05163564229804	21.077208921723507
36	23.780841181457323	29.940610544428758	26.510715660557153	19.767832613556763
37	21.80788907377061	30.912387800050066	27.245944814558488	20.033778311620836
38	22.381037025194633	30.87036869529365	26.37445596040212	20.374138319109594
39	22.184956634601026	29.947105362247804	26.688652632951815	21.17928537019936
40	23.57085083691118	27.527562218777017	26.89498799904918	22.006598945262624
41	20.3977773523862	27.6170960433675	27.667398012953875	24.317728591292422
42	21.622615849669423	27.930424987220725	26.185711821515046	24.26124734159481
43	21.220725989158122	27.718778069708588	27.202873917442716	23.857622023690574
44	21.38307013982528	27.92595486969344	27.56277096800658	23.128204022474698
45	21.35535541115615	29.4880768818143	25.87921952799766	23.277348179031886
46	21.73089787306549	29.971612124220886	26.56288456158139	21.734605441132235
47	21.736524962188064	28.434496475443805	27.49976860568094	22.32920995668719
48	21.940888217611633	27.629822730915755	29.06404679213848	21.36524225933413
49	21.981487402682912	27.299296981987002	28.587532263730626	22.13168335159946
50	21.273710029260865	28.653716297943326	28.414433536189023	21.65814013660679
51	19.872775196211865	29.05544838960072	27.71020596197981	23.361570452207605
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2341.0
1	3493.0
2	4645.0
3	25668.5
4	46692.0
5	31808.5
6	16925.0
7	16944.0
8	16963.0
9	19123.5
10	21284.0
11	22331.5
12	23379.0
13	23411.5
14	23444.0
15	21969.5
16	20495.0
17	19385.0
18	18275.0
19	17658.0
20	17041.0
21	16422.5
22	15804.0
23	16964.5
24	18125.0
25	19754.5
26	26218.5
27	31053.0
28	40100.5
29	49148.0
30	56376.5
31	63605.0
32	73596.0
33	83587.0
34	92524.0
35	101461.0
36	118380.5
37	135300.0
38	149023.5
39	162747.0
40	185467.5
41	208188.0
42	228690.5
43	249193.0
44	257225.5
45	265258.0
46	275412.5
47	285567.0
48	286422.0
49	287277.0
50	287388.5
51	287500.0
52	273700.5
53	259901.0
54	244580.5
55	229260.0
56	215911.0
57	202562.0
58	192431.5
59	182301.0
60	164053.0
61	145805.0
62	131089.0
63	116373.0
64	97729.0
65	79085.0
66	61963.0
67	44841.0
68	35094.0
69	25347.0
70	20692.0
71	16037.0
72	13301.5
73	10566.0
74	8958.0
75	5657.0
76	3964.0
77	2804.0
78	1644.0
79	1111.5
80	579.0
81	490.0
82	401.0
83	283.5
84	166.0
85	123.5
86	81.0
87	60.5
88	40.0
89	29.0
90	18.0
91	17.5
92	17.0
93	14.0
94	11.0
95	6.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3803032.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.147788654377294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.38321128489602	15.391607410433448
2	7.652357908861826	2.9305146389307763
3	2.5140536925539925	1.4441570631234202
4	1.285081134350462	0.9842584785708032
5	0.8359942038251087	0.8003720165563796
6	0.526441397321498	0.6048113168896264
7	0.41175566504614675	0.551894731608233
8	0.310470780639717	0.47558631128390677
9	0.26658572379484075	0.4594074387748029
>10	3.141290281061656	14.622783855966654
>50	1.3087381499070005	18.111373263124243
>100	1.358874981728809	42.16190905233771
>500	0.004588601849527678	0.5529422002769709
>1k	4.1714562268433433E-4	0.1283311539548825
>5k	0.0	0.0
>10k+	1.390485408947781E-4	0.7800510681680805
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29298	0.7703853136129278	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02324461114184682	0.0
2	0.0	0.0	0.0	0.07909478542384077	0.0
3	0.0	0.0	0.0	0.1157497491475223	0.0
4	0.0	0.0	0.0	0.20767640135555	0.0
5	0.0	0.0	0.0	0.3806962444702017	0.0
6	0.0	0.0	0.0	0.6468260061971606	0.0
7	0.0	0.0	0.0	0.7928936701032229	0.0
8	0.0	0.0	0.0	1.0921811859589927	0.0
9	0.0	0.0	0.0	1.1994114169957024	0.0
10	0.0	0.0	0.0	1.3888655157253476	0.0
11	2.6294808983989616E-5	0.0	0.0	1.608137927842837	0.0
12	2.6294808983989616E-5	0.0	0.0	1.7736374555880676	0.0
13	2.6294808983989616E-5	0.0	0.0	1.8519959863603568	0.0
14	2.6294808983989616E-5	0.0	0.0	1.8875728629156947	0.0
15	2.6294808983989616E-5	0.0	0.0	1.9293027247732861	0.0
16	2.6294808983989616E-5	0.0	0.0	2.0278293740362954	0.0
17	2.6294808983989616E-5	0.0	0.0	2.1463663729361206	0.0
18	2.6294808983989616E-5	0.0	0.0	2.2882268674047443	0.0
19	2.6294808983989616E-5	0.0	0.0	2.3761567086472057	0.0
20	2.6294808983989616E-5	0.0	0.0	2.4726323628094637	0.0
21	2.6294808983989616E-5	0.0	0.0	2.6054737377965793	0.0
22	2.6294808983989616E-5	0.0	0.0	2.757931040285751	0.0
23	2.6294808983989616E-5	0.0	0.0	2.925902280075477	0.0
24	2.6294808983989616E-5	0.0	0.0	3.051486287783011	0.0
25	2.6294808983989616E-5	0.0	0.0	3.170259939963692	0.0
26	5.258961796797923E-5	0.0	0.0	3.2791204491574093	0.0
27	5.258961796797923E-5	0.0	0.0	3.3928980876311323	0.0
28	5.258961796797923E-5	0.0	0.0	3.519849425405834	0.0
29	5.258961796797923E-5	0.0	0.0	3.6564509580776603	0.0
30	5.258961796797923E-5	0.0	0.0	3.8145090548804217	0.0
31	5.258961796797923E-5	0.0	0.0	3.9619177540446677	0.0
32	5.258961796797923E-5	0.0	0.0	4.106591793074578	0.0
33	5.258961796797923E-5	0.0	0.0	4.256261845811447	0.0
34	5.258961796797923E-5	0.0	0.0	4.408140662502971	0.0
35	5.258961796797923E-5	0.0	0.0	4.571326247057611	0.0
36	5.258961796797923E-5	0.0	0.0	4.737193902128618	0.0
37	5.258961796797923E-5	0.0	0.0	4.902746019491816	0.0
38	5.258961796797923E-5	0.0	0.0	5.090964262199214	0.0
39	5.258961796797923E-5	0.0	0.0	5.332587261953094	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATC	25	3.8925114E-5	45.0	44
TTACGCG	110	0.0	42.954544	1
CGTTTTT	14910	0.0	42.751507	1
TCGTCCC	1485	0.0	40.60606	38
AGGGCGA	3395	0.0	40.427097	6
CTCGTCC	1515	0.0	39.653465	37
GGGCGAT	6230	0.0	39.47432	7
CGTAAGG	665	0.0	39.24812	2
GGCGATA	1175	0.0	38.87234	8
CCGCGAA	35	6.252094E-6	38.57143	15
TAATCGT	270	0.0	38.333336	21
TACGGGT	765	0.0	38.235294	4
AGGGTAC	2250	0.0	38.2	6
CGTTAGG	655	0.0	38.129772	2
TAGTGCG	290	0.0	38.017242	1
TATACGG	385	0.0	37.987015	2
CGCATCG	90	0.0	37.5	21
GGCGATT	1855	0.0	37.479786	8
GTAGGGA	4230	0.0	37.39362	4
TAGGGCA	2510	0.0	37.290836	5
>>END_MODULE