FastQCFastQC Report
Thu 26 May 2016
SRR1547415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547415_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2680645
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251720.9390277339968552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT138100.042.2302671
GTCAACG1000.040.51
TAGGGAC27500.039.190915
TACGCGG1100.038.8636362
CGTTAGG2850.038.684212
GGCGATA8600.038.45938
GGGCGAT39500.038.3924067
AGGGCGA23550.037.8343966
CGGTCTA1550.037.7419431
TCGAATA301.1403275E-437.50000445
CATACGA5050.037.42574318
CGTAAGG4400.037.3295442
TTAGGGA33900.037.0353974
GGCGATT11550.037.0129858
CACTCGA552.752131E-936.8181839
CGTATGG3500.036.6428572
GCGATAT2400.036.5625049
TAAGGGA32400.036.388894
AAGGGAT35850.036.3389135
TATGGGA30400.036.2664454
Produced by FastQC (version 0.11.5)