##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547415_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2680645
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.280830919424243	31.0	31.0	33.0	30.0	34.0
2	31.696377550925245	31.0	31.0	34.0	30.0	34.0
3	31.79913341751705	31.0	31.0	34.0	30.0	34.0
4	35.58596457195936	37.0	35.0	37.0	33.0	37.0
5	35.475386707303656	37.0	35.0	37.0	33.0	37.0
6	35.51345254593577	37.0	35.0	37.0	33.0	37.0
7	35.9168882862147	37.0	35.0	37.0	35.0	37.0
8	35.89137726181572	37.0	35.0	37.0	35.0	37.0
9	37.60030626957318	39.0	37.0	39.0	35.0	39.0
10	36.874587645883736	39.0	37.0	39.0	32.0	39.0
11	36.60423144429792	39.0	35.0	39.0	32.0	39.0
12	36.225760591200995	38.0	35.0	39.0	32.0	39.0
13	36.06208244657536	38.0	35.0	39.0	31.0	39.0
14	37.13990886521714	39.0	35.0	41.0	31.0	41.0
15	37.272019980265945	39.0	35.0	41.0	32.0	41.0
16	37.33822941866603	39.0	35.0	41.0	32.0	41.0
17	37.26143558733066	39.0	35.0	41.0	32.0	41.0
18	37.10696604735055	39.0	35.0	41.0	32.0	41.0
19	37.05535085772267	39.0	35.0	41.0	31.0	41.0
20	36.903144579009904	39.0	35.0	41.0	31.0	41.0
21	36.78769437952433	38.0	35.0	41.0	31.0	41.0
22	36.7059371904896	38.0	35.0	40.0	31.0	41.0
23	36.603784909975026	38.0	35.0	40.0	31.0	41.0
24	36.46381076196214	38.0	35.0	40.0	30.0	41.0
25	36.386133188094654	38.0	35.0	40.0	30.0	41.0
26	36.244938438323615	38.0	35.0	40.0	30.0	41.0
27	36.155865845719966	38.0	35.0	40.0	30.0	41.0
28	36.21727569297688	38.0	35.0	40.0	30.0	41.0
29	36.23077169860239	38.0	35.0	40.0	30.0	41.0
30	36.16834120146457	38.0	35.0	40.0	30.0	41.0
31	36.03465770364968	38.0	35.0	40.0	30.0	41.0
32	35.91555502500331	38.0	35.0	40.0	29.0	41.0
33	35.68290057057163	38.0	34.0	40.0	28.0	41.0
34	35.42918439405442	38.0	34.0	40.0	27.0	41.0
35	35.25063594769169	38.0	34.0	40.0	26.0	41.0
36	35.10649339990935	38.0	34.0	40.0	25.0	41.0
37	35.01355793102033	38.0	34.0	40.0	25.0	41.0
38	34.923209526065556	38.0	34.0	40.0	24.0	41.0
39	34.86497801835006	38.0	34.0	40.0	24.0	41.0
40	34.703515758334284	38.0	34.0	40.0	24.0	41.0
41	34.61222914634351	37.0	33.0	40.0	23.0	41.0
42	34.539260886838804	37.0	33.0	40.0	23.0	41.0
43	34.48152515532642	37.0	33.0	40.0	23.0	41.0
44	34.360820250350194	37.0	33.0	40.0	23.0	41.0
45	34.276169727808046	37.0	33.0	40.0	23.0	41.0
46	34.18816553478734	37.0	33.0	40.0	23.0	41.0
47	34.03464166273415	36.0	33.0	40.0	23.0	41.0
48	33.978904703905215	36.0	33.0	40.0	23.0	41.0
49	33.85942301199898	36.0	33.0	40.0	22.0	41.0
50	33.796523224820895	36.0	33.0	40.0	22.0	41.0
51	33.34001742118035	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	8.0
13	20.0
14	45.0
15	111.0
16	272.0
17	701.0
18	1497.0
19	2852.0
20	4797.0
21	7658.0
22	11506.0
23	16934.0
24	23621.0
25	34097.0
26	45931.0
27	54055.0
28	58885.0
29	64960.0
30	74860.0
31	88494.0
32	107387.0
33	135569.0
34	205291.0
35	260689.0
36	220147.0
37	271950.0
38	386937.0
39	601215.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.4758780069722	24.133594713212677	27.828265212290326	15.562262067524793
2	31.46634485357069	25.74589324584195	29.301343519936434	13.486418380650925
3	28.153709275193094	25.30058996995126	32.901372617411106	13.644328137444534
4	24.761204859278273	27.98762984281768	32.636921337961574	14.614243959942478
5	22.12157894835012	32.21235933889046	32.122418298581124	13.543643414178305
6	21.91002538568143	40.23770398542142	28.069363903090487	9.782906725806662
7	88.4466984624969	2.9091505962184474	7.452982397893044	1.191168543391609
8	88.69936153425762	2.473919523099851	7.410418015067269	1.4163009275752665
9	83.72425293166384	5.141449166152176	8.920502341787143	2.213795560396845
10	39.071939775688314	32.29625705753653	16.595259722939815	12.036543443835345
11	31.95689843302638	25.324502125421304	25.91648651723746	16.80211292431486
12	30.36205838520207	21.958260045623348	29.22057937548612	18.45910219368846
13	24.081853434527883	26.363804233682565	30.779905582425126	18.774436749364426
14	20.00335740092403	28.14781517134869	31.701735962799997	20.147091464927282
15	19.210525824941385	26.72188969445786	36.20464477765613	17.862939702944626
16	23.471701773267252	24.512570668626392	34.1159310539068	17.89979650419955
17	23.287231244719088	25.234561085111977	29.67699937888083	21.801208291288106
18	23.08168369925895	25.58943836278209	32.067170401153454	19.26170753680551
19	25.577389023910293	27.2099811799026	28.373245991170037	18.839383805017075
20	26.332281969451383	27.019765765328867	29.416950025087246	17.231002240132508
21	25.864894456371506	26.249204948808963	30.66702230246825	17.21887829235128
22	25.045315586360744	23.910625987402288	30.425102913664436	20.618955512572533
23	22.11303622822119	26.98925072137489	30.472293048874434	20.42542000152948
24	22.12751035664924	25.772304799777668	32.80893217863611	19.291252664936984
25	22.06413008809447	27.50733498840764	30.575775606244022	19.85275931725387
26	20.966856857211607	29.08016540795219	29.121349525953644	20.831628208882563
27	20.417287630402384	29.02570090407346	31.18189838639581	19.375113079128344
28	19.02974843740965	28.19847462084685	32.70347248516682	20.068304456576683
29	20.98771004739531	27.050094287009287	31.927651740532596	20.034543925062813
30	21.983776292645988	27.257320532931438	31.1984615642877	19.560441610134873
31	23.920810103538514	27.243853624780602	29.366141357770235	19.469194913910645
32	25.017411854236578	26.928892113651752	29.22229538040285	18.831400651708822
33	24.35786909493797	27.327676734517254	28.600206293634557	19.71424787691022
34	21.407795511901053	28.10599687761714	30.234924803545415	20.25128280693639
35	21.260032566788965	28.151545616819835	30.25040615225067	20.33801566414053
36	24.051226477209774	28.40025441638113	27.80103296035096	19.747486146058133
37	21.89204463851051	30.444799665752086	28.204741769238375	19.458413926499034
38	22.725015807762684	30.047171482982645	26.956422801228808	20.271389908025867
39	21.30293268970714	29.817487955324186	28.04492948525448	20.83464986971419
40	23.256529678491557	27.6113771125979	27.8958981886822	21.23619502022834
41	20.166564390286666	27.32607264296466	29.151155785268095	23.356207181480578
42	21.39541043293685	27.913282064577743	28.194893393194548	22.49641410929086
43	21.168935088383577	27.537588901178633	28.73789703597455	22.555578974463238
44	20.444743709070018	28.62669245647969	28.814333863678332	22.11422997077196
45	20.79320462052976	29.891425384562297	27.407470963145066	21.907899031762877
46	21.784831635669775	29.76802224837679	27.471112362882817	20.976033753070624
47	20.990619794862802	28.618783912080858	29.08445542024401	21.306140872812325
48	21.41797962803728	27.631111169140265	29.975658843300774	20.975250359521684
49	21.72186171611683	27.299847611302503	30.07500060619739	20.903290066383278
50	20.994984416064042	28.705777900467982	28.84447586308519	21.45476182038278
51	19.5276509944435	29.212185873176043	28.396337448636427	22.863825683744025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1870.0
1	3055.0
2	4240.0
3	22709.5
4	41179.0
5	28362.0
6	15545.0
7	15486.5
8	15428.0
9	16381.5
10	17335.0
11	17694.5
12	18054.0
13	17797.0
14	17540.0
15	17017.0
16	16494.0
17	15439.0
18	14384.0
19	13955.5
20	13527.0
21	14658.0
22	15789.0
23	16859.0
24	17929.0
25	21185.5
26	28914.5
27	33387.0
28	40981.0
29	48575.0
30	54469.5
31	60364.0
32	66458.0
33	72552.0
34	81482.5
35	90413.0
36	98451.0
37	106489.0
38	114379.0
39	122269.0
40	133090.0
41	143911.0
42	157500.0
43	171089.0
44	178267.5
45	185446.0
46	185301.5
47	185157.0
48	183381.5
49	181606.0
50	175667.0
51	169728.0
52	163991.0
53	158254.0
54	148946.0
55	139638.0
56	135544.5
57	131451.0
58	121322.5
59	111194.0
60	104182.5
61	97171.0
62	86548.0
63	75925.0
64	65301.5
65	54678.0
66	46840.5
67	39003.0
68	32095.5
69	25188.0
70	21525.0
71	17862.0
72	14506.0
73	11150.0
74	8632.5
75	5125.5
76	4136.0
77	3221.5
78	2307.0
79	1762.0
80	1217.0
81	776.5
82	336.0
83	243.5
84	151.0
85	106.5
86	62.0
87	47.0
88	32.0
89	21.5
90	11.0
91	11.0
92	11.0
93	9.5
94	8.0
95	5.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2680645.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.908587480625876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.286349825711	17.589605186567216
2	6.7819972345797686	2.9716795941430725
3	2.3386980583531436	1.5371271300659919
4	1.273789945264005	1.1162775379103238
5	0.8840424835022774	0.9684061043199701
6	0.646432452635544	0.8497453163328816
7	0.4728377125574953	0.7251444472793442
8	0.395575075742347	0.6933192921645134
9	0.3295479243680188	0.6497936577067878
>10	4.16937647989375	21.822659290098674
>50	1.5735982903113086	24.906340342900066
>100	0.8463785829635171	24.974435765554436
>500	6.879670590202113E-4	0.09812123609112917
>1k	5.159752942651585E-4	0.1488398166512904
>5k	0.0	0.0
>10k+	1.7199176475505282E-4	0.9485052822142487
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25172	0.9390277339968552	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.460890942291874E-5	0.0	0.0	0.01827918280861509	0.0
2	7.460890942291874E-5	0.0	0.0	0.05938869190064332	0.0
3	7.460890942291874E-5	0.0	0.0	0.09139591404307545	0.0
4	7.460890942291874E-5	0.0	0.0	0.1628712492702316	0.0
5	1.1191336413437811E-4	0.0	0.0	0.3022406920722438	0.0
6	1.1191336413437811E-4	0.0	0.0	0.5174127868479415	0.0
7	1.1191336413437811E-4	0.0	0.0	0.6352202548267302	0.0
8	1.1191336413437811E-4	0.0	0.0	0.8691937947770033	0.0
9	1.1191336413437811E-4	0.0	0.0	0.9748027060651447	0.0
10	1.1191336413437811E-4	0.0	0.0	1.1579302742436988	0.0
11	1.1191336413437811E-4	0.0	0.0	1.3545247505730897	0.0
12	1.1191336413437811E-4	0.0	0.0	1.5079579728013222	0.0
13	1.1191336413437811E-4	0.0	0.0	1.5844694094145253	0.0
14	1.1191336413437811E-4	0.0	0.0	1.6226318665843482	0.0
15	1.1191336413437811E-4	0.0	0.0	1.6674345166928108	0.0
16	1.1191336413437811E-4	0.0	0.0	1.752861717982053	0.0
17	1.1191336413437811E-4	0.0	0.0	1.8537702679765504	0.0
18	1.1191336413437811E-4	0.0	0.0	1.9753454858811965	0.0
19	1.1191336413437811E-4	0.0	0.0	2.0531998828640123	0.0
20	1.1191336413437811E-4	0.0	0.0	2.139858131158732	0.0
21	1.1191336413437811E-4	0.0	0.0	2.2637089208007772	0.0
22	1.1191336413437811E-4	0.0	0.0	2.395057905839826	0.0
23	1.1191336413437811E-4	0.0	0.0	2.5484538236133467	0.0
24	1.1191336413437811E-4	0.0	0.0	2.6616355392079143	0.0
25	1.1191336413437811E-4	0.0	0.0	2.7680278440449966	0.0
26	1.1191336413437811E-4	0.0	0.0	2.870391267773241	0.0
27	1.1191336413437811E-4	0.0	0.0	2.9736873028692723	0.0
28	1.1191336413437811E-4	0.0	0.0	3.0842577066340375	0.0
29	1.1191336413437811E-4	0.0	0.0	3.211204766017134	0.0
30	1.1191336413437811E-4	0.0	0.0	3.348932812811842	0.0
31	1.1191336413437811E-4	0.0	0.0	3.481363627037523	0.0
32	1.1191336413437811E-4	0.0	0.0	3.6124887853483023	0.0
33	1.1191336413437811E-4	0.0	0.0	3.7424948100177384	0.0
34	1.1191336413437811E-4	0.0	0.0	3.8819761661838847	0.0
35	1.1191336413437811E-4	0.0	0.0	4.025635621277715	0.0
36	1.1191336413437811E-4	0.0	0.0	4.1690339451885645	0.0
37	1.1191336413437811E-4	0.0	0.0	4.318550199672094	0.0
38	1.1191336413437811E-4	0.0	0.0	4.48716633496789	0.0
39	1.1191336413437811E-4	0.0	0.0	4.7296079861376645	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	13810	0.0	42.230267	1
GTCAACG	100	0.0	40.5	1
TAGGGAC	2750	0.0	39.19091	5
TACGCGG	110	0.0	38.863636	2
CGTTAGG	285	0.0	38.68421	2
GGCGATA	860	0.0	38.4593	8
GGGCGAT	3950	0.0	38.392406	7
AGGGCGA	2355	0.0	37.834396	6
CGGTCTA	155	0.0	37.74194	31
TCGAATA	30	1.1403275E-4	37.500004	45
CATACGA	505	0.0	37.425743	18
CGTAAGG	440	0.0	37.329544	2
TTAGGGA	3390	0.0	37.035397	4
GGCGATT	1155	0.0	37.012985	8
CACTCGA	55	2.752131E-9	36.81818	39
CGTATGG	350	0.0	36.642857	2
GCGATAT	240	0.0	36.562504	9
TAAGGGA	3240	0.0	36.38889	4
AAGGGAT	3585	0.0	36.338913	5
TATGGGA	3040	0.0	36.266445	4
>>END_MODULE