FastQCFastQC Report
Thu 26 May 2016
SRR1547414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547414_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1830981
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367222.0055915380880522No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAT207.0343824E-445.00000415
CGTGGCA207.0343824E-445.00000412
AGACTCG207.0343824E-445.00000437
TCGCATA207.0343824E-445.00000412
ACGTTAG302.1662108E-645.0000041
ATCCGAA302.1662108E-645.00000411
ACGATTC207.0343824E-445.00000424
GTTACGA302.1662108E-645.00000418
CACTACG302.1662108E-645.0000041
ACTACGC207.0343824E-445.00000422
ACAGCGT207.0343824E-445.00000425
TGTTACG650.045.0000041
TCGTACT207.0343824E-445.00000444
TTACGAT302.1662108E-645.00000419
TAATGCG800.045.0000041
AGTACGC406.8193913E-945.00000412
GCGTAAT406.8193913E-945.00000411
GTACGAT207.0343824E-445.00000426
CGCATAT253.891716E-545.013
CGCGACA253.891716E-545.019