##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547414_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1830981
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.345273380772383	31.0	31.0	33.0	30.0	34.0
2	31.750916038997673	31.0	31.0	34.0	30.0	34.0
3	31.74704925938609	31.0	31.0	34.0	30.0	34.0
4	35.57871873056028	37.0	35.0	37.0	33.0	37.0
5	35.49084616388701	37.0	35.0	37.0	33.0	37.0
6	35.53363306336876	37.0	35.0	37.0	33.0	37.0
7	35.95603340504353	37.0	35.0	37.0	35.0	37.0
8	35.95677453780241	37.0	35.0	37.0	35.0	37.0
9	37.693920909064595	39.0	37.0	39.0	35.0	39.0
10	36.97340332859817	39.0	37.0	39.0	32.0	39.0
11	36.67364653155877	39.0	35.0	39.0	32.0	39.0
12	36.15417909852697	38.0	35.0	39.0	32.0	39.0
13	35.88532759214869	38.0	35.0	39.0	31.0	39.0
14	36.99981813028098	39.0	35.0	41.0	31.0	41.0
15	37.215009877218826	39.0	35.0	41.0	32.0	41.0
16	37.3256101510611	39.0	35.0	41.0	32.0	41.0
17	37.2656297361906	39.0	35.0	41.0	32.0	41.0
18	37.11796353976366	39.0	35.0	40.0	32.0	41.0
19	36.9731662971926	38.0	35.0	40.0	32.0	41.0
20	36.744949838365336	38.0	35.0	40.0	32.0	41.0
21	36.607995932235234	38.0	35.0	40.0	31.0	41.0
22	36.51696658785645	38.0	35.0	40.0	31.0	41.0
23	36.42869368933921	38.0	35.0	40.0	31.0	41.0
24	36.265564743708424	38.0	35.0	40.0	31.0	41.0
25	36.17233603188673	37.0	35.0	40.0	31.0	41.0
26	35.979437798644554	37.0	35.0	40.0	30.0	41.0
27	35.867218720456414	37.0	35.0	40.0	30.0	41.0
28	35.90304978587981	37.0	35.0	40.0	30.0	41.0
29	35.90031791700733	37.0	35.0	40.0	30.0	41.0
30	35.81940719210085	37.0	35.0	40.0	30.0	41.0
31	35.6423070474243	37.0	34.0	40.0	30.0	41.0
32	35.41622332509185	37.0	34.0	40.0	29.0	41.0
33	35.08607790031682	37.0	34.0	40.0	27.0	41.0
34	34.748197824008	37.0	34.0	40.0	24.0	41.0
35	34.48329775131473	36.0	34.0	40.0	23.0	41.0
36	34.25413207455457	36.0	33.0	40.0	23.0	41.0
37	34.110315726924526	36.0	33.0	40.0	22.0	41.0
38	34.04412771077362	36.0	33.0	40.0	22.0	41.0
39	34.02176210457673	36.0	33.0	40.0	22.0	41.0
40	33.86369601869162	36.0	33.0	40.0	21.0	41.0
41	33.79771335693817	36.0	33.0	40.0	21.0	41.0
42	33.70660809697097	36.0	33.0	40.0	20.0	41.0
43	33.6200954570255	35.0	33.0	40.0	20.0	41.0
44	33.49679871063654	35.0	33.0	40.0	19.0	41.0
45	33.41542429986985	35.0	33.0	40.0	20.0	41.0
46	33.33701005089621	35.0	33.0	40.0	20.0	41.0
47	33.21121082086597	35.0	33.0	40.0	19.0	41.0
48	33.15749535358368	35.0	33.0	40.0	18.0	41.0
49	33.06005578430361	35.0	32.0	40.0	18.0	41.0
50	32.93856135044547	35.0	32.0	39.0	18.0	41.0
51	32.47456636633586	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	4.0
13	10.0
14	14.0
15	67.0
16	183.0
17	420.0
18	953.0
19	1936.0
20	3371.0
21	5558.0
22	8375.0
23	13242.0
24	19493.0
25	29152.0
26	39581.0
27	43727.0
28	44453.0
29	46048.0
30	51714.0
31	60734.0
32	74459.0
33	95455.0
34	154208.0
35	229819.0
36	161379.0
37	165220.0
38	224337.0
39	356917.0
40	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.702864748459977	24.187634934496863	29.373270394395135	17.736229922648022
2	34.53329117014322	25.63379958612351	27.623388773559093	12.209520470174185
3	26.298088292560106	24.306915254718646	35.62085024366719	13.774146209054052
4	23.59642180885547	26.504480385105033	35.51593380816076	14.383163997878734
5	21.49694617257088	30.91326452868708	34.81882116745068	12.770968131291369
6	19.761810745168848	39.6447041230903	31.13183588469787	9.46164924704298
7	83.86624437937914	3.634226679577778	11.236708627779315	1.262820313263764
8	84.49694453410494	2.870210013102266	10.86985610445985	1.7629893483329429
9	80.47816989908688	5.16979695583952	12.239832090010765	2.1122010550628323
10	41.987983490817214	26.05291917283686	20.67711243317107	11.281984903174855
11	35.27338623393689	25.29785945348422	24.531002779384384	14.8977515331945
12	31.724250552026483	23.384076623405704	28.74513716963748	16.146535654930336
13	20.03374147519827	35.121445826035334	29.471687581684353	15.373125117082045
14	16.400716337307706	36.79470185654575	30.72828172438709	16.076300081759452
15	15.517692428266598	29.085828853494384	40.489879468984114	14.906599249254906
16	16.79383893115221	26.217694230579124	40.353722949610074	16.634743888658594
17	16.845887532421145	26.901535297198603	30.580983636640685	25.67159353373956
18	19.38976974638186	29.196152226593284	33.41771432909462	17.996363697930235
19	22.527595862545816	31.317747153028897	28.144857865810728	18.009799118614556
20	25.012820995957902	28.81488120302723	29.68649046603979	16.485807334975075
21	20.3316145825653	31.175582925218777	31.02888560831598	17.46391688389994
22	21.242055488287427	28.81772121065156	28.580143649770264	21.360079651290757
23	17.975172871810248	32.92977917302255	27.93726423157859	21.15778372358861
24	18.004064487834665	29.960441970724982	36.041881373973844	15.993612167466512
25	16.692144811988765	31.56335319700204	33.79188533359986	17.952616657409333
26	16.883790711099678	36.122275435954826	28.97856395014476	18.01536990280074
27	16.88335378684978	36.21807107774466	30.305939821330753	16.592635314074805
28	15.596174946654278	33.26271545144379	34.86180359053426	16.279306011367677
29	15.911634255079655	29.670051191137432	35.00418628046933	19.414128273313594
30	17.171833022844037	33.9523457643744	31.77050990698429	17.105311305797276
31	22.33414765090408	32.220104960127934	28.638691499256407	16.80705588971158
32	22.94633314054051	31.007257857946097	29.84187165240928	16.204537349104115
33	22.318090684720378	31.9465357641614	27.610991047968277	18.12438250314995
34	18.469552660568297	32.60732907659883	28.91133223119191	20.011786031640963
35	18.476598064097878	31.400325836259363	30.47989028832085	19.64318581132191
36	24.501783470172548	31.209389939054528	26.89093988413861	17.397886706634313
37	19.048804984868767	34.980373908849955	29.370703464426995	16.600117641854283
38	19.068302729520404	35.208557598358475	27.039712591228415	18.6834270808927
39	18.834329793700753	35.14913590037253	27.86134864315905	18.155185662767664
40	21.48498537122996	31.554560096472873	26.640800751072785	20.319653781224382
41	17.176366111936716	30.579618248359758	28.8173389019329	23.426676737770627
42	19.84007480143158	31.314306374560957	26.78848114753785	22.05713767646961
43	20.103321661994308	31.804644614007465	27.309240237883408	20.78279348611482
44	18.37501317599691	33.9631050240281	27.448673689131674	20.213208110843315
45	17.303292606531688	37.249758462813105	25.288137888924023	20.15881104173118
46	20.464166476877697	35.856680107548904	25.839317830168635	17.839835585404764
47	19.572021774119992	32.625952972750675	27.96517276804074	19.836852485088592
48	19.89927803729258	30.527296569434636	30.575576699048213	18.997848694224572
49	20.000808309862308	30.527624262622062	29.203634554372766	20.267932873142865
50	18.56491137810824	34.37954844971084	28.11154239175611	18.94399778042481
51	17.046053454405044	35.22991227107217	26.37209233738635	21.35194193713643
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1995.0
1	2491.0
2	2987.0
3	24686.0
4	46385.0
5	30400.0
6	14415.0
7	15735.0
8	17055.0
9	20687.5
10	24320.0
11	27521.0
12	30722.0
13	31637.5
14	32553.0
15	29872.5
16	27192.0
17	24592.0
18	21992.0
19	20220.5
20	18449.0
21	17489.5
22	16530.0
23	16577.5
24	16625.0
25	17097.5
26	19477.0
27	21384.0
28	23295.0
29	25206.0
30	31163.5
31	37121.0
32	41265.5
33	45410.0
34	50244.5
35	55079.0
36	57893.5
37	60708.0
38	69228.0
39	77748.0
40	92241.0
41	106734.0
42	126906.0
43	147078.0
44	151804.0
45	156530.0
46	153687.5
47	150845.0
48	147264.5
49	143684.0
50	134501.0
51	125318.0
52	113325.0
53	101332.0
54	88371.5
55	75411.0
56	67148.5
57	58886.0
58	53384.0
59	47882.0
60	42918.5
61	37955.0
62	30839.5
63	23724.0
64	19588.5
65	15453.0
66	13327.0
67	11201.0
68	9260.0
69	7319.0
70	5767.5
71	4216.0
72	3501.0
73	2786.0
74	2059.0
75	1152.5
76	973.0
77	741.0
78	509.0
79	341.5
80	174.0
81	137.5
82	101.0
83	76.0
84	51.0
85	37.5
86	24.0
87	14.5
88	5.0
89	4.5
90	4.0
91	3.5
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1830981.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.0381276562879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.34513445004822	19.866536052656343
2	7.8669609926270745	3.9394794720086805
3	3.0434281584638514	2.286052282330773
4	1.7437291450824774	1.7463885173025933
5	1.1563417098649564	1.44763156729445
6	0.8110266984908444	1.2183954005682887
7	0.59003454609071	1.0341352200647358
8	0.44118501360800727	0.8837157352606718
9	0.3731133220460152	0.8407853088884807
>10	2.9906341140343606	16.42692118228898
>50	0.8076066961223001	14.565624381283213
>100	0.8268562513403123	32.942380605977824
>500	0.0028519849083598683	0.4627261871163997
>1k	8.775338179568826E-4	0.3221066430734292
>5k	0.0	0.0
>10k+	2.1938345448922064E-4	2.017121443885141
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36722	2.0055915380880522	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027471612212251247	0.0
2	0.0	0.0	0.0	0.18421818686267089	0.0
3	0.0	0.0	0.0	0.3008769615850738	0.0
4	0.0	0.0	0.0	0.49263209175846173	0.0
5	0.0	0.0	0.0	0.8914893163828571	0.0
6	0.0	0.0	0.0	1.386961415765647	0.0
7	0.0	0.0	0.0	1.6626060019191897	0.0
8	0.0	0.0	0.0	2.109033354251082	0.0
9	0.0	0.0	0.0	2.302317719299108	0.0
10	0.0	0.0	0.0	2.6254232020976733	0.0
11	0.0	0.0	0.0	3.1422499741941614	0.0
12	0.0	0.0	0.0	3.4856724346129204	0.0
13	0.0	0.0	0.0	3.6495736438553976	0.0
14	0.0	0.0	0.0	3.7188261374640152	0.0
15	0.0	0.0	0.0	3.806047140849632	0.0
16	0.0	0.0	0.0	3.989555325806221	0.0
17	0.0	0.0	0.0	4.216755935752474	0.0
18	0.0	0.0	0.0	4.461706593350778	0.0
19	0.0	0.0	0.0	4.606219289004091	0.0
20	0.0	0.0	0.0	4.74259427050308	0.0
21	0.0	0.0	0.0	4.945709431173781	0.0
22	0.0	0.0	0.0	5.158054616623548	0.0
23	0.0	0.0	0.0	5.402131425722058	0.0
24	0.0	0.0	0.0	5.586841152365863	0.0
25	0.0	0.0	0.0	5.7441884978598905	0.0
26	0.0	0.0	0.0	5.888865040106915	0.0
27	0.0	0.0	0.0	6.028790031136315	0.0
28	0.0	0.0	0.0	6.184171217505807	0.0
29	0.0	0.0	0.0	6.348727813123129	0.0
30	0.0	0.0	0.0	6.5475283468261	0.0
31	0.0	0.0	0.0	6.7286334484082575	0.0
32	0.0	0.0	0.0	6.900890833930008	0.0
33	0.0	0.0	0.0	7.073748990295366	0.0
34	0.0	0.0	0.0	7.252724086159278	0.0
35	0.0	0.0	0.0	7.453490778986784	0.0
36	0.0	0.0	0.0	7.641477437504813	0.0
37	0.0	0.0	0.0	7.827661783492019	0.0
38	0.0	0.0	0.0	8.017887678790768	0.0
39	5.461553123708001E-5	0.0	0.0	8.21592359505642	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAT	20	7.0343824E-4	45.000004	15
CGTGGCA	20	7.0343824E-4	45.000004	12
AGACTCG	20	7.0343824E-4	45.000004	37
TCGCATA	20	7.0343824E-4	45.000004	12
ACGTTAG	30	2.1662108E-6	45.000004	1
ATCCGAA	30	2.1662108E-6	45.000004	11
ACGATTC	20	7.0343824E-4	45.000004	24
GTTACGA	30	2.1662108E-6	45.000004	18
CACTACG	30	2.1662108E-6	45.000004	1
ACTACGC	20	7.0343824E-4	45.000004	22
ACAGCGT	20	7.0343824E-4	45.000004	25
TGTTACG	65	0.0	45.000004	1
TCGTACT	20	7.0343824E-4	45.000004	44
TTACGAT	30	2.1662108E-6	45.000004	19
TAATGCG	80	0.0	45.000004	1
AGTACGC	40	6.8193913E-9	45.000004	12
GCGTAAT	40	6.8193913E-9	45.000004	11
GTACGAT	20	7.0343824E-4	45.000004	26
CGCATAT	25	3.891716E-5	45.0	13
CGCGACA	25	3.891716E-5	45.0	19
>>END_MODULE