FastQCFastQC Report
Thu 26 May 2016
SRR1547413_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547413_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1868982
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243031.3003335505638898No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT24420.1306593642956433No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22100.11824618963692535No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG900.045.0000041
TACCCGC207.0344214E-445.029
GATCGAC406.8193913E-945.09
CTATACG351.2124838E-745.01
CACGCTA207.0344214E-445.030
CAATTCG207.0344214E-445.043
TAACGCG406.8193913E-945.01
CGTTCGA253.8917475E-545.014
CGTTCCA207.0344214E-445.031
CCCGTTG207.0344214E-445.011
AATGCGT207.0344214E-445.025
AACGCGT253.8917475E-545.042
ACTACGT207.0344214E-445.013
GCGATCG406.8193913E-945.09
ACGCTAG502.1827873E-1145.01
TTAACGC207.0344214E-445.022
CGTACAC207.0344214E-445.026
CGTCAAT406.8193913E-945.012
GTACGAC207.0344214E-445.034
TCGCACC302.1662345E-644.99999645