##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547413_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1868982
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.348779174973327	31.0	31.0	33.0	30.0	34.0
2	31.759144282823485	31.0	31.0	34.0	30.0	34.0
3	31.851724093650983	31.0	31.0	34.0	30.0	34.0
4	35.64030365193458	37.0	35.0	37.0	33.0	37.0
5	35.52578516004969	37.0	35.0	37.0	33.0	37.0
6	35.57321900371432	37.0	35.0	37.0	33.0	37.0
7	35.94556929922278	37.0	35.0	37.0	35.0	37.0
8	35.92580720413573	37.0	35.0	37.0	35.0	37.0
9	37.64896933196788	39.0	37.0	39.0	35.0	39.0
10	36.97507145601188	39.0	37.0	39.0	32.0	39.0
11	36.70184785086213	39.0	35.0	39.0	32.0	39.0
12	36.213967282723964	38.0	35.0	39.0	32.0	39.0
13	35.95946830948613	38.0	35.0	39.0	31.0	39.0
14	37.08451445760312	39.0	35.0	41.0	31.0	41.0
15	37.29454804808179	39.0	35.0	41.0	32.0	41.0
16	37.40112157313447	39.0	35.0	41.0	32.0	41.0
17	37.337433961375766	39.0	35.0	41.0	32.0	41.0
18	37.23758334751218	39.0	35.0	41.0	32.0	41.0
19	37.14985965621927	38.0	35.0	41.0	32.0	41.0
20	36.96919231966921	38.0	35.0	41.0	32.0	41.0
21	36.85025644976784	38.0	35.0	41.0	31.0	41.0
22	36.74517946133243	38.0	35.0	41.0	31.0	41.0
23	36.64909667401826	38.0	35.0	40.0	31.0	41.0
24	36.51308412815105	38.0	35.0	40.0	31.0	41.0
25	36.42350648641881	38.0	35.0	40.0	31.0	41.0
26	36.277881220899935	38.0	35.0	40.0	31.0	41.0
27	36.16343389074908	38.0	35.0	40.0	30.0	41.0
28	36.22969670119883	38.0	35.0	40.0	30.0	41.0
29	36.259610311923815	38.0	35.0	40.0	30.0	41.0
30	36.19926195115844	38.0	35.0	40.0	30.0	41.0
31	36.079964387029946	38.0	35.0	40.0	30.0	41.0
32	35.92406133392403	38.0	35.0	40.0	30.0	41.0
33	35.70159798221706	38.0	35.0	40.0	29.0	41.0
34	35.497798801700604	38.0	34.0	40.0	28.0	41.0
35	35.3440552129448	38.0	34.0	40.0	27.0	41.0
36	35.146618854542204	38.0	34.0	40.0	26.0	41.0
37	35.03116990960854	38.0	34.0	40.0	25.0	41.0
38	34.9804690467859	37.0	34.0	40.0	25.0	41.0
39	34.95407018366148	37.0	34.0	40.0	25.0	41.0
40	34.779838971161844	37.0	34.0	40.0	24.0	41.0
41	34.72260674527631	37.0	34.0	40.0	24.0	41.0
42	34.6312719972691	37.0	34.0	40.0	24.0	41.0
43	34.563785526024326	37.0	33.0	40.0	24.0	41.0
44	34.42683129104507	37.0	33.0	40.0	23.0	41.0
45	34.35289424938282	36.0	33.0	40.0	23.0	41.0
46	34.25716994599199	36.0	33.0	40.0	23.0	41.0
47	34.1509490193057	36.0	33.0	40.0	23.0	41.0
48	34.10430062996861	36.0	33.0	40.0	23.0	41.0
49	33.98009451134361	36.0	33.0	40.0	23.0	41.0
50	33.89827349862117	36.0	33.0	40.0	23.0	41.0
51	33.4676058945458	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	7.0
13	18.0
14	28.0
15	74.0
16	181.0
17	378.0
18	832.0
19	1626.0
20	2877.0
21	4638.0
22	7111.0
23	10493.0
24	15080.0
25	21952.0
26	30285.0
27	35571.0
28	38887.0
29	42917.0
30	49079.0
31	60011.0
32	73498.0
33	95101.0
34	151994.0
35	218331.0
36	156416.0
37	177275.0
38	251464.0
39	422682.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.543481959697843	24.026181097517256	29.120077132899088	15.31025980988581
2	31.250755759017473	25.815925461026378	29.5636876117587	13.369631168197445
3	27.71326850659878	25.554071681803247	33.73815264138445	12.994507170213518
4	24.770757556787597	28.164851239872828	33.32386293714975	13.74052826618983
5	22.8009151506007	32.39587112128421	31.03395324299538	13.769260485119709
6	21.264303241015696	41.138116900002245	27.545262608200616	10.05231725078144
7	87.50983155536008	3.4660044880047	7.65598598595385	1.3681779706813657
8	87.73540890174438	3.156103162042224	7.384073254852106	1.724414681361297
9	83.19748397790883	5.981812558922451	8.744225466055854	2.0764779971128666
10	40.29899699408555	34.005356926926	15.420961785613773	10.274684293374682
11	34.880378730239244	24.019278944366505	26.07291027949975	15.027432045894503
12	34.07122165970566	22.779727145579788	26.621390682200257	16.5276605125143
13	21.508660864577614	33.27404972332532	26.89234032216469	18.324949089932378
14	16.276989291496655	34.86133092774569	29.120933213910032	19.74074656684762
15	15.824871507590762	29.53051447258454	37.80485847375737	16.839755546067327
16	17.86764131489763	25.732404057395954	38.39266509789822	18.007289529808208
17	17.86052514149414	26.77109784898945	28.973901300280048	26.394475709236364
18	19.625817691128113	29.40263737157447	31.69848612774227	19.27305880955515
19	23.46523401509485	30.271399082495176	26.279493328453675	19.983873573956306
20	26.007955132794216	27.07730732559222	29.302261873041047	17.612475668572518
21	21.798069751340567	30.851179947158396	29.203438021339963	18.14731228016107
22	23.09310630064923	27.55425145881555	26.725672050346123	22.6269701901891
23	19.85396328054524	32.184258596390976	27.44799040333187	20.51378771973192
24	19.039081168250952	27.972500537725885	35.57931537061352	17.40910292340964
25	17.76913849357565	31.254394103314	31.261563781780673	19.714903621329686
26	18.446726613739457	34.2652845238745	27.803585053253588	19.48440380913246
27	17.974223400760415	35.13827313478674	28.250726866283355	18.636776598169487
28	16.30256471169867	31.747068725113458	33.87822889680051	18.07213766638737
29	17.258646685735872	27.59887468151111	33.42386390024088	21.718614732512137
30	17.68609863551388	33.302139881496984	30.583815146427305	18.427946336561828
31	23.542655841522283	31.422400001712163	26.530218054534497	18.504726102231054
32	23.483639756830186	30.31645034569621	27.51096586269959	18.68894403477401
33	22.846661979623132	31.08430150745165	26.343057343516417	19.7259791694088
34	18.479311197218593	29.7855196037201	28.35918162935759	23.37598756970372
35	19.78462071865861	28.971012026868102	29.933728628740138	21.31063862573315
36	23.883911134510658	30.824587930755886	26.278369722126804	19.01313121260665
37	19.47536145345434	33.82012239818254	28.65843544774642	18.0460807006167
38	19.313936677827822	33.0216128352226	27.408182636322877	20.256267850626706
39	19.388576240969684	33.14911540079037	27.32728298078847	20.13502537745147
40	22.109148188693094	30.603130474236778	26.832842691903934	20.45487864516619
41	17.25126298701646	28.61959077187474	29.92907368824312	24.200072552865677
42	19.69692592009982	29.41799332470832	26.96248545999908	23.922595295192785
43	20.95996644162437	30.557116119898424	26.7072128035476	21.775704634929603
44	18.607348813418216	32.05263614095802	27.417171486937807	21.92284355868596
45	17.841316823811038	35.36764934065711	24.53463971295604	22.25639412257582
46	19.313348122132798	35.25106180797889	25.693987422029746	19.741602647858567
47	19.7765414541178	30.680284775348294	28.056556991988153	21.486616778545752
48	20.038555748530484	28.663946469254387	30.27626804324493	21.0212297389702
49	19.4559391155185	29.611574643308497	29.6351703761727	21.297315865000304
50	18.58022174638386	31.91844544249222	29.47674188408449	20.024590927039426
51	17.522854687739102	32.40598357822601	26.9114951347846	23.159666599250286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1636.0
1	2526.0
2	3416.0
3	18019.5
4	32623.0
5	21566.0
6	10509.0
7	11315.5
8	12122.0
9	14090.5
10	16059.0
11	17882.0
12	19705.0
13	19867.5
14	20030.0
15	18932.5
16	17835.0
17	16474.5
18	15114.0
19	14093.5
20	13073.0
21	12803.0
22	12533.0
23	12742.5
24	12952.0
25	14825.0
26	19801.5
27	22905.0
28	26108.0
29	29311.0
30	34921.0
31	40531.0
32	46189.0
33	51847.0
34	63248.0
35	74649.0
36	76220.5
37	77792.0
38	81578.0
39	85364.0
40	99431.0
41	113498.0
42	128411.5
43	143325.0
44	148917.0
45	154509.0
46	148622.5
47	142736.0
48	137854.5
49	132973.0
50	127669.5
51	122366.0
52	110715.5
53	99065.0
54	90489.0
55	81913.0
56	75386.0
57	68859.0
58	64970.0
59	61081.0
60	56873.0
61	52665.0
62	44964.0
63	37263.0
64	31994.0
65	26725.0
66	20882.5
67	15040.0
68	12980.5
69	10921.0
70	9502.5
71	8084.0
72	6129.5
73	4175.0
74	3447.0
75	2365.0
76	2011.0
77	1534.5
78	1058.0
79	742.5
80	427.0
81	469.5
82	512.0
83	358.5
84	205.0
85	143.0
86	81.0
87	57.0
88	33.0
89	24.5
90	16.0
91	12.0
92	8.0
93	7.5
94	7.0
95	3.5
96	0.0
97	1.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1868982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.009683093221188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.41295042563887	16.894506050335785
2	7.753265867524305	3.2578731762834856
3	2.8049263729141	1.767918425842308
4	1.446202585448903	1.2153703203551434
5	0.9066641730901711	0.952436347430097
6	0.5867655813548491	0.7396655348565045
7	0.44831511913399363	0.6593271005233436
8	0.3506883351701844	0.5894280629131925
9	0.2698715869014196	0.5102924864997176
>10	2.7090582892771646	13.250789896546678
>50	1.0513339161641373	16.074672986376363
>100	1.2535736890765097	41.66597539529289
>500	0.004596521979899649	0.6114583583649946
>1k	0.0015321739932998833	0.5064100591916456
>5k	0.0	0.0
>10k+	2.5536233221664723E-4	1.3038757991878973
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24303	1.3003335505638898	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2442	0.1306593642956433	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2210	0.11824618963692535	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.3505063184129116E-5	0.0	0.0	0.03895168599804599	0.0
2	5.3505063184129116E-5	0.0	0.0	0.14473119591306927	0.0
3	5.3505063184129116E-5	0.0	0.0	0.20166058314098262	0.0
4	5.3505063184129116E-5	0.0	0.0	0.3294841790878671	0.0
5	5.3505063184129116E-5	0.0	0.0	0.5831516836438232	0.0
6	5.3505063184129116E-5	0.0	0.0	0.9331818069943959	0.0
7	5.3505063184129116E-5	0.0	0.0	1.1489142217528046	0.0
8	5.3505063184129116E-5	0.0	0.0	1.5435675677989409	0.0
9	5.3505063184129116E-5	0.0	0.0	1.682841247267229	0.0
10	5.3505063184129116E-5	0.0	0.0	1.9005533493634503	0.0
11	5.3505063184129116E-5	0.0	0.0	2.2110432310209513	0.0
12	5.3505063184129116E-5	0.0	0.0	2.4529396216763995	0.0
13	5.3505063184129116E-5	0.0	0.0	2.5674404568904357	0.0
14	5.3505063184129116E-5	0.0	0.0	2.6101910023745547	0.0
15	5.3505063184129116E-5	0.0	0.0	2.665836268086049	0.0
16	5.3505063184129116E-5	0.0	0.0	2.7862761653135237	0.0
17	5.3505063184129116E-5	0.0	0.0	2.9281715928778342	0.0
18	5.3505063184129116E-5	0.0	0.0	3.102972634300384	0.0
19	5.3505063184129116E-5	0.0	0.0	3.205488335361175	0.0
20	5.3505063184129116E-5	0.0	0.0	3.306559399715995	0.0
21	5.3505063184129116E-5	0.0	0.0	3.45032750449175	0.0
22	5.3505063184129116E-5	0.0	0.0	3.598215499132683	0.0
23	5.3505063184129116E-5	0.0	0.0	3.768147579805477	0.0
24	5.3505063184129116E-5	0.0	0.0	3.8935099428458915	0.0
25	5.3505063184129116E-5	0.0	0.0	4.00881335400769	0.0
26	5.3505063184129116E-5	0.0	0.0	4.116519046197341	0.0
27	5.3505063184129116E-5	0.0	0.0	4.222245051049181	0.0
28	5.3505063184129116E-5	0.0	0.0	4.339207119169687	0.0
29	5.3505063184129116E-5	0.0	0.0	4.464462472083733	0.0
30	5.3505063184129116E-5	0.0	0.0	4.611815416092825	0.0
31	5.3505063184129116E-5	0.0	0.0	4.749002398096932	0.0
32	5.3505063184129116E-5	0.0	0.0	4.878056610497051	0.0
33	5.3505063184129116E-5	0.0	0.0	5.0140129760479235	0.0
34	5.3505063184129116E-5	0.0	0.0	5.146438007428643	0.0
35	5.3505063184129116E-5	0.0	0.0	5.312143188109891	0.0
36	5.3505063184129116E-5	0.0	0.0	5.449811715682655	0.0
37	5.3505063184129116E-5	0.0	0.0	5.602033620441502	0.0
38	5.3505063184129116E-5	0.0	0.0	5.750724191030197	0.0
39	5.3505063184129116E-5	0.0	0.0	5.907815056538801	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTAG	90	0.0	45.000004	1
TACCCGC	20	7.0344214E-4	45.0	29
GATCGAC	40	6.8193913E-9	45.0	9
CTATACG	35	1.2124838E-7	45.0	1
CACGCTA	20	7.0344214E-4	45.0	30
CAATTCG	20	7.0344214E-4	45.0	43
TAACGCG	40	6.8193913E-9	45.0	1
CGTTCGA	25	3.8917475E-5	45.0	14
CGTTCCA	20	7.0344214E-4	45.0	31
CCCGTTG	20	7.0344214E-4	45.0	11
AATGCGT	20	7.0344214E-4	45.0	25
AACGCGT	25	3.8917475E-5	45.0	42
ACTACGT	20	7.0344214E-4	45.0	13
GCGATCG	40	6.8193913E-9	45.0	9
ACGCTAG	50	2.1827873E-11	45.0	1
TTAACGC	20	7.0344214E-4	45.0	22
CGTACAC	20	7.0344214E-4	45.0	26
CGTCAAT	40	6.8193913E-9	45.0	12
GTACGAC	20	7.0344214E-4	45.0	34
TCGCACC	30	2.1662345E-6	44.999996	45
>>END_MODULE