FastQCFastQC Report
Thu 26 May 2016
SRR1547412_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547412_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3822819
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235100.6149911884397351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT126850.041.5944021
TACGGGA11250.040.44
ATAGGGT43150.039.889924
CGTTCGA800.039.37500414
CGTTAGG7100.039.2957762
AAGGGTA42150.038.914595
TAGGGCG12200.038.9139335
CGCAATA356.2521067E-638.57142628
GGGCGAT61600.038.2426957
ACGGGAC8700.038.017245
TAGGGTA39950.038.016275
TAGGGTC30450.037.9802935
ACGGGTA13250.037.5282975
TAGGGAC31150.037.415735
GCGTAAG2050.037.3170741
AGGGTAC34500.037.3043486
TAGGGAT44900.037.182635
TAAGGGT37700.037.1816984
TATGGGT34450.037.097244
TACGGGT13150.036.9581764
Produced by FastQC (version 0.11.5)