##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547412_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3822819
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25584104295809	31.0	31.0	33.0	30.0	34.0
2	31.68733779967087	31.0	31.0	34.0	30.0	34.0
3	31.862434240281843	31.0	31.0	34.0	30.0	34.0
4	35.645169703300105	37.0	35.0	37.0	33.0	37.0
5	35.51824295107877	37.0	35.0	37.0	33.0	37.0
6	35.54866500349611	37.0	35.0	37.0	33.0	37.0
7	35.93090517756661	37.0	35.0	37.0	35.0	37.0
8	35.90779631470912	37.0	35.0	37.0	35.0	37.0
9	37.605300957225545	39.0	37.0	39.0	35.0	39.0
10	36.63814766014295	39.0	35.0	39.0	32.0	39.0
11	36.55770231339752	39.0	35.0	39.0	32.0	39.0
12	36.34213076789668	38.0	35.0	39.0	32.0	39.0
13	36.25142231426599	39.0	35.0	39.0	32.0	39.0
14	37.35580340057952	39.0	36.0	41.0	32.0	41.0
15	37.48560237876813	39.0	36.0	41.0	32.0	41.0
16	37.527859937914926	39.0	36.0	41.0	32.0	41.0
17	37.42743614071187	39.0	36.0	41.0	32.0	41.0
18	37.30164546111129	39.0	36.0	41.0	32.0	41.0
19	37.283289373627156	39.0	36.0	41.0	32.0	41.0
20	37.1692844992138	39.0	35.0	41.0	32.0	41.0
21	37.059316959552625	39.0	35.0	41.0	31.0	41.0
22	36.97676557535159	39.0	35.0	41.0	31.0	41.0
23	36.86385675073814	39.0	35.0	41.0	31.0	41.0
24	36.73817724563993	38.0	35.0	40.0	31.0	41.0
25	36.66587641214507	38.0	35.0	40.0	31.0	41.0
26	36.55622146902587	38.0	35.0	40.0	30.0	41.0
27	36.47792506001461	38.0	35.0	40.0	30.0	41.0
28	36.552616537691165	38.0	35.0	40.0	30.0	41.0
29	36.58615958537404	38.0	35.0	41.0	30.0	41.0
30	36.53524035535033	38.0	35.0	41.0	30.0	41.0
31	36.45295029662665	38.0	35.0	41.0	30.0	41.0
32	36.37299150182104	38.0	35.0	41.0	30.0	41.0
33	36.201709523783364	38.0	35.0	41.0	30.0	41.0
34	36.000424032631415	38.0	35.0	41.0	29.0	41.0
35	35.86476733530936	38.0	35.0	40.0	29.0	41.0
36	35.735706555816535	38.0	35.0	40.0	27.0	41.0
37	35.656418731831145	38.0	35.0	40.0	27.0	41.0
38	35.564772226987465	38.0	34.0	40.0	27.0	41.0
39	35.5303873921313	38.0	34.0	40.0	27.0	41.0
40	35.36808334373142	38.0	34.0	40.0	26.0	41.0
41	35.29011418013775	38.0	34.0	40.0	26.0	41.0
42	35.223633397239055	38.0	34.0	40.0	26.0	41.0
43	35.16928292969141	38.0	34.0	40.0	26.0	41.0
44	35.045867983809856	38.0	34.0	40.0	25.0	41.0
45	34.953642063618496	38.0	34.0	40.0	25.0	41.0
46	34.86968255624972	38.0	34.0	40.0	24.0	41.0
47	34.73285473364028	37.0	33.0	40.0	24.0	41.0
48	34.68503347922044	37.0	33.0	40.0	24.0	41.0
49	34.56361234994385	37.0	33.0	40.0	24.0	41.0
50	34.49375395486943	37.0	33.0	40.0	24.0	41.0
51	34.056945149639574	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	7.0
12	7.0
13	22.0
14	44.0
15	108.0
16	324.0
17	747.0
18	1716.0
19	3300.0
20	5553.0
21	8745.0
22	13378.0
23	19454.0
24	27450.0
25	38683.0
26	53057.0
27	64751.0
28	73860.0
29	83646.0
30	98503.0
31	118329.0
32	145391.0
33	183001.0
34	271856.0
35	349516.0
36	310775.0
37	398503.0
38	596114.0
39	955731.0
40	248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.612111638034655	24.282499380692624	29.00922068243357	14.096168298839155
2	30.398954279551294	26.19485777380514	29.696854598661353	13.709333347982207
3	28.12704446640032	26.159752789760642	31.80603109903974	13.907171644799297
4	25.04774617893235	28.85198069801369	31.00337735058866	15.096895772465293
5	23.080192915228263	33.06570360773032	29.697194661845096	14.156908815196326
6	22.351332877648666	41.843911521837676	25.7019492683279	10.102806332185752
7	90.88162426732734	2.6247646043404096	5.436616277150448	1.0569948511818112
8	91.45410755779963	2.000460916407499	5.408338715487184	1.1370928103056934
9	86.05625848359549	4.1465996689877285	7.916043108501867	1.881098738914921
10	33.403046285999935	28.0007240729943	29.07697173211706	9.519257908888703
11	31.83543348507999	25.559855174937656	24.695179133513776	17.90953220646858
12	29.358465572134072	23.490387590937473	28.128535512667487	19.02261132426097
13	24.21472217230269	26.749082287181263	28.60276670174549	20.433428838770553
14	20.15094096790876	28.866472621382282	29.87711424474975	21.105472165959206
15	20.314040502571533	27.404724105431093	33.41952627105809	18.861709120939285
16	23.46786494469134	25.990976815800064	32.21164800112169	18.329510238386906
17	23.820431990109917	25.37459921591893	28.55314363562596	22.25182515834519
18	23.33819623686081	26.05817853264829	30.963354529733163	19.64027070075774
19	25.27901530258168	27.407261499955922	27.095135814695908	20.218587382766486
20	26.777595277202504	26.6459908250953	28.3181338169555	18.258280080746694
21	25.930027029791365	26.50604174563326	30.00429787546834	17.559633349107035
22	25.60932128881854	23.422688858666863	30.156515388251442	20.811474464263153
23	22.8907515631789	26.087528601275658	29.827072639327156	21.194647196218288
24	21.814765491120557	26.15745082359379	33.14096220616252	18.886821479123128
25	21.79179814686492	28.26163101104185	29.85045852288586	20.09611231920737
26	21.69930096088776	29.624578092763482	27.465778526265566	21.21034242008319
27	20.389953068664774	29.21501122600887	29.869711330826803	20.52532437449955
28	19.31412394884508	28.670046894713035	31.650177526061263	20.365651630380615
29	20.416059457693393	26.391440452713038	31.4631950924174	21.72930499717617
30	21.374331350764972	27.816776049297655	31.004999190388034	19.80389340954934
31	23.450757150678598	27.764589429946852	28.81904688660384	19.96560653277071
32	23.886587358700478	26.827166025909154	28.869036174613548	20.41721044077682
33	23.495959395409514	27.860591882587173	27.780180018985988	20.86326870301733
34	20.88874728309135	27.75446601055399	30.15452732656189	21.202259379792764
35	21.484485663590142	27.80076692095545	29.594704850007286	21.12004256544712
36	23.56467831723134	29.072524752022005	27.3765511785936	19.98624575215306
37	22.052966671976883	29.33235918310545	28.43278742728861	20.18188671762906
38	21.66636714947791	29.316585483121226	28.333985993059045	20.68306137434181
39	21.638429650998386	29.50775330979573	27.890517442756245	20.963299596449637
40	23.517043312801363	27.30414387916352	28.21996019168054	20.95885261635458
41	20.661846663417755	27.156922679310743	29.14890817483119	23.032322482440314
42	21.746281997656702	27.112792941543923	28.08961135748253	23.051313703316843
43	21.534370316774087	27.288579448830824	28.286743369225693	22.890306865169393
44	20.941195489506566	28.349262677620885	28.332050248782377	22.37749158409017
45	21.10432118287578	29.403824769103636	27.30338527667671	22.18846877134387
46	21.931328687023896	28.762413287157983	27.831974257740168	21.474283768077957
47	21.372290971662537	28.2816424214696	29.064049331134957	21.28201727573291
48	21.35426762292434	27.33754854728932	30.17085035938139	21.137333470404958
49	21.72292227280444	27.733617521520117	29.371204862171084	21.17225534350436
50	21.029507282453082	29.037132022206645	29.09112359230191	20.84223710303836
51	20.608064362974023	28.796027224935315	28.298907167720994	22.297001244369667
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2179.0
1	3709.5
2	5240.0
3	22359.5
4	39479.0
5	27643.0
6	15807.0
7	15747.0
8	15687.0
9	16304.5
10	16922.0
11	17359.0
12	17796.0
13	17883.5
14	17971.0
15	17295.0
16	16619.0
17	15247.0
18	13875.0
19	15174.5
20	16474.0
21	16374.0
22	16274.0
23	19934.0
24	23594.0
25	27808.5
26	37391.5
27	42760.0
28	52843.0
29	62926.0
30	73112.5
31	83299.0
32	95942.5
33	108586.0
34	124235.5
35	139885.0
36	151098.5
37	162312.0
38	169368.5
39	176425.0
40	202610.5
41	228796.0
42	250266.0
43	271736.0
44	275531.5
45	279327.0
46	278640.5
47	277954.0
48	277870.0
49	277786.0
50	270698.5
51	263611.0
52	249452.0
53	235293.0
54	221123.0
55	206953.0
56	193521.0
57	180089.0
58	165837.5
59	151586.0
60	137617.0
61	123648.0
62	112121.5
63	100595.0
64	85922.0
65	71249.0
66	59361.5
67	47474.0
68	38320.5
69	29167.0
70	24794.5
71	20422.0
72	16544.5
73	12667.0
74	10460.5
75	6552.5
76	4851.0
77	3783.5
78	2716.0
79	2011.0
80	1306.0
81	1035.0
82	764.0
83	473.0
84	182.0
85	139.5
86	97.0
87	112.5
88	128.0
89	72.5
90	17.0
91	13.5
92	10.0
93	7.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3822819.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.77821638048255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.890417429032	14.558655605413703
2	6.720856744697705	2.3896969093892273
3	2.0121908673617366	1.0731949391636346
4	1.0024179448508312	0.712848125089468
5	0.6689104998900286	0.5946017803110839
6	0.4418488659296958	0.4713170847581374
7	0.34857398351581564	0.433791659248565
8	0.26273992086570896	0.37368377319531487
9	0.2348629810473703	0.3757900407142789
>10	3.243263036977309	14.602596636180623
>50	1.6110637138850117	20.893887509782523
>100	1.5597337726795515	42.40481085390775
>500	0.0022287423338327246	0.255859328495661
>1k	7.429141112775748E-4	0.23824027294057057
>5k	0.0	0.0
>10k+	1.4858282225551495E-4	0.6210254814095592
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23510	0.6149911884397351	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0463482576601194E-4	0.0	0.0	0.014701193020124678	0.0
2	1.0463482576601194E-4	0.0	0.0	0.052578999947421	0.0
3	1.0463482576601194E-4	0.0	0.0	0.08012411783032364	0.0
4	1.0463482576601194E-4	0.0	0.0	0.1559058903913578	0.0
5	1.0463482576601194E-4	0.0	0.0	0.2820431728522852	0.0
6	1.0463482576601194E-4	0.0	0.0	0.4687640194317335	0.0
7	1.0463482576601194E-4	0.0	0.0	0.5580436845165831	0.0
8	1.0463482576601194E-4	0.0	0.0	0.753475380341052	0.0
9	1.0463482576601194E-4	0.0	0.0	0.8120185653571357	0.0
10	1.0463482576601194E-4	0.0	0.0	0.9266983343966847	0.0
11	1.3079353220751494E-4	0.0	0.0	1.065941128784805	0.0
12	1.3079353220751494E-4	0.0	0.0	1.1752845217102876	0.0
13	1.3079353220751494E-4	0.0	0.0	1.2239135569850417	0.0
14	1.3079353220751494E-4	0.0	0.0	1.2464100445247341	0.0
15	1.3079353220751494E-4	0.0	0.0	1.278899157925081	0.0
16	1.3079353220751494E-4	0.0	0.0	1.350155474271735	0.0
17	1.3079353220751494E-4	0.0	0.0	1.4301749572762927	0.0
18	1.3079353220751494E-4	0.0	0.0	1.5262035686230502	0.0
19	1.3079353220751494E-4	0.0	0.0	1.5845636426940433	0.0
20	1.3079353220751494E-4	0.0	0.0	1.6504312655137479	0.0
21	1.3079353220751494E-4	0.0	0.0	1.7374351231381868	0.0
22	1.3079353220751494E-4	0.0	0.0	1.8395587130858144	0.0
23	1.3079353220751494E-4	0.0	0.0	1.944219697558268	0.0
24	1.3079353220751494E-4	0.0	0.0	2.0268027337940926	0.0
25	1.3079353220751494E-4	0.0	0.0	2.109045706846178	0.0
26	1.5695223864901792E-4	0.0	0.0	2.1840427182139672	0.0
27	1.5695223864901792E-4	0.0	0.0	2.259955284307209	5.231741288300597E-5
28	1.5695223864901792E-4	0.0	0.0	2.3380390230350954	5.231741288300597E-5
29	1.831109450905209E-4	0.0	0.0	2.4296729716996803	5.231741288300597E-5
30	1.831109450905209E-4	0.0	0.0	2.540271982534355	5.231741288300597E-5
31	1.831109450905209E-4	0.0	0.0	2.6352280869170106	5.231741288300597E-5
32	1.831109450905209E-4	0.0	0.0	2.731492126621742	5.231741288300597E-5
33	1.831109450905209E-4	0.0	0.0	2.8296134344838193	5.231741288300597E-5
34	1.831109450905209E-4	0.0	0.0	2.932207881147394	5.231741288300597E-5
35	1.831109450905209E-4	0.0	0.0	3.053584279035968	5.231741288300597E-5
36	1.831109450905209E-4	0.0	0.0	3.1616982127587	5.231741288300597E-5
37	1.831109450905209E-4	0.0	0.0	3.274494554934461	5.231741288300597E-5
38	1.831109450905209E-4	0.0	0.0	3.403116914507331	5.231741288300597E-5
39	1.831109450905209E-4	0.0	0.0	3.5815454511448226	5.231741288300597E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12685	0.0	41.594402	1
TACGGGA	1125	0.0	40.4	4
ATAGGGT	4315	0.0	39.88992	4
CGTTCGA	80	0.0	39.375004	14
CGTTAGG	710	0.0	39.295776	2
AAGGGTA	4215	0.0	38.91459	5
TAGGGCG	1220	0.0	38.913933	5
CGCAATA	35	6.2521067E-6	38.571426	28
GGGCGAT	6160	0.0	38.242695	7
ACGGGAC	870	0.0	38.01724	5
TAGGGTA	3995	0.0	38.01627	5
TAGGGTC	3045	0.0	37.980293	5
ACGGGTA	1325	0.0	37.528297	5
TAGGGAC	3115	0.0	37.41573	5
GCGTAAG	205	0.0	37.317074	1
AGGGTAC	3450	0.0	37.304348	6
TAGGGAT	4490	0.0	37.18263	5
TAAGGGT	3770	0.0	37.181698	4
TATGGGT	3445	0.0	37.09724	4
TACGGGT	1315	0.0	36.958176	4
>>END_MODULE