FastQCFastQC Report
Thu 26 May 2016
SRR1547411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547411_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2785597
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT514191.8458879730269668No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29440.1056865009547325No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCG207.0350175E-445.00000411
CGTTTTT231300.043.69651
CGTACAA650.041.53846437
TACGCGG2200.039.8863642
GCGATAC1700.039.7058839
CGCTATC403.460591E-739.37500444
ATAGGGT16700.039.3413164
GGCGATA11300.039.2256628
TCGATAG750.039.0000041
TACGGGA5950.038.949584
GGGCGAT44200.038.789597
GGTACGC356.251492E-638.571438
CGTATAG700.038.5714314
GTTTTTT268650.038.5259632
TATTGCG2350.038.297871
TAGGGCG11650.038.047215
CACGACG1850.037.702726
ATTGGGC11550.037.59744
TGGGCGA14500.037.5517236
CGCGAAC301.140339E-437.50000444
Produced by FastQC (version 0.11.5)