##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547411_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2785597
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.341611510925667	31.0	31.0	33.0	30.0	34.0
2	31.75219387441902	31.0	31.0	34.0	30.0	34.0
3	31.781412386644586	31.0	31.0	34.0	30.0	34.0
4	35.613299052231895	37.0	35.0	37.0	33.0	37.0
5	35.53260288548559	37.0	35.0	37.0	33.0	37.0
6	35.56878615248365	37.0	35.0	37.0	33.0	37.0
7	35.966149087610304	37.0	35.0	37.0	35.0	37.0
8	35.95357978917984	37.0	35.0	37.0	35.0	37.0
9	37.7007295025088	39.0	37.0	39.0	35.0	39.0
10	36.9219887155249	39.0	37.0	39.0	32.0	39.0
11	36.72107200000574	39.0	35.0	39.0	32.0	39.0
12	36.33199490091352	38.0	35.0	39.0	32.0	39.0
13	36.15166838562793	38.0	35.0	39.0	32.0	39.0
14	37.26171517272599	39.0	35.0	41.0	32.0	41.0
15	37.41056477300916	39.0	35.0	41.0	32.0	41.0
16	37.48635247668633	39.0	35.0	41.0	32.0	41.0
17	37.40998895389391	39.0	35.0	41.0	32.0	41.0
18	37.23442586993021	39.0	35.0	41.0	32.0	41.0
19	37.10159258500063	38.0	35.0	41.0	32.0	41.0
20	36.892367776099704	38.0	35.0	41.0	32.0	41.0
21	36.760105643422214	38.0	35.0	40.0	31.0	41.0
22	36.67218804442997	38.0	35.0	40.0	31.0	41.0
23	36.595197008038134	38.0	35.0	40.0	31.0	41.0
24	36.45330390576957	38.0	35.0	40.0	31.0	41.0
25	36.34506606662773	38.0	35.0	40.0	31.0	41.0
26	36.18942294955085	38.0	35.0	40.0	30.0	41.0
27	36.08696053305629	38.0	35.0	40.0	30.0	41.0
28	36.125464308010095	38.0	35.0	40.0	30.0	41.0
29	36.113791406294595	38.0	35.0	40.0	30.0	41.0
30	36.02171132435883	38.0	35.0	40.0	30.0	41.0
31	35.86746108643856	38.0	35.0	40.0	30.0	41.0
32	35.66839065378086	38.0	34.0	40.0	29.0	41.0
33	35.33763354857145	38.0	34.0	40.0	27.0	41.0
34	34.99062678485079	38.0	34.0	40.0	25.0	41.0
35	34.72772694686274	38.0	34.0	40.0	24.0	41.0
36	34.51932206991894	37.0	34.0	40.0	23.0	41.0
37	34.38508405918013	37.0	33.0	40.0	23.0	41.0
38	34.29899874245987	37.0	33.0	40.0	22.0	41.0
39	34.24204865240736	37.0	33.0	40.0	22.0	41.0
40	34.077710092306965	37.0	33.0	40.0	21.0	41.0
41	33.991930993607475	37.0	33.0	40.0	21.0	41.0
42	33.92036249321061	37.0	33.0	40.0	21.0	41.0
43	33.858189106320836	37.0	33.0	40.0	20.0	41.0
44	33.72285402375146	36.0	33.0	40.0	20.0	41.0
45	33.62196110923439	36.0	33.0	40.0	20.0	41.0
46	33.53720333558659	36.0	33.0	40.0	20.0	41.0
47	33.39697486750596	36.0	33.0	40.0	20.0	41.0
48	33.351812914789896	36.0	33.0	40.0	20.0	41.0
49	33.2414918597342	36.0	32.0	40.0	18.0	41.0
50	33.15380473198385	35.0	32.0	40.0	18.0	41.0
51	32.72607380033795	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	3.0
11	3.0
12	9.0
13	14.0
14	32.0
15	99.0
16	222.0
17	670.0
18	1534.0
19	3028.0
20	5213.0
21	8321.0
22	12609.0
23	18833.0
24	28126.0
25	42015.0
26	58469.0
27	65979.0
28	67266.0
29	69659.0
30	76391.0
31	90259.0
32	109267.0
33	139721.0
34	215393.0
35	299165.0
36	239796.0
37	267976.0
38	379784.0
39	585574.0
40	166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.791674818719287	23.170903759589056	28.650483181881658	18.386938239809993
2	32.95753118631302	25.928014712824577	29.1844082255976	11.930045875264799
3	26.885583234042826	24.71969204447018	36.17917451806561	12.215550203421385
4	23.554950698180676	27.228777170567025	35.36556077566138	13.850711355590919
5	22.132060021603987	31.011449251273604	34.0036265116598	12.852864215462608
6	20.01093481935829	39.53012585811946	30.95314936080129	9.505789961720952
7	85.17965807688621	2.957678371997098	10.73245699216362	1.1302065589530719
8	85.84831186995103	2.4124810588179124	10.345933026205872	1.3932740450251777
9	82.08592269448883	4.305899238116641	11.709698136521544	1.8984799308729872
10	37.91155001961877	24.708204381322926	24.30304886169823	13.077196737360069
11	33.0758182177824	25.46046682273136	25.593939108923507	15.869775850562734
12	29.816480991327893	23.45113094248737	28.830013817504828	17.902374248679905
13	22.109730876361514	29.754734801911404	29.752939854544646	18.38259446718244
14	18.280569658855896	31.880921755731357	31.011664644957616	18.826843940455134
15	18.39052813454351	27.124418930663698	37.67497595668002	16.81007697811277
16	20.4051770589931	24.918859404285687	37.03260019306454	17.643363343656674
17	20.3530158885151	26.138813331576678	30.29648581614641	23.211684963761808
18	21.145377454096913	27.64416389018225	33.099691017760286	18.11076763796055
19	23.906329594697297	28.404252302109747	29.246190313961424	18.443227789231535
20	25.6003650204965	27.922919216239823	29.530007391593255	16.946708371670418
21	22.637481301135807	28.795048242800377	31.40615817722377	17.161312278840047
22	22.626137233777893	25.860596489729133	30.86810475456428	20.645161521928692
23	20.70489737029441	29.210829850836284	29.66635159357222	20.417921185297082
24	20.009642457254227	27.760655974284866	35.03561355070385	17.194088017757057
25	19.318264630526237	28.876179863777857	32.961695464203906	18.843860041492004
26	19.14160591068988	32.0887048629073	29.545982423157408	19.223706803245406
27	18.347485296688646	32.603926555061626	30.583785091669757	18.464803056579974
28	17.484474602751224	30.516295070679643	33.974943252738996	18.024287073830134
29	18.616619704860394	28.46567540100022	33.73700502980151	19.18069986433788
30	19.07601853390853	30.646321057927615	32.30600119112707	17.971659217036777
31	22.190072720497618	29.46355844007586	29.956989471197737	18.389379368228788
32	23.160816155387877	28.95214203633907	29.96793865013496	17.919103158138093
33	23.094941587027844	29.729713235618792	28.238399165421274	18.936946011932093
34	19.54274074821304	29.982836713279053	30.078435610032606	20.395986928475295
35	20.087866263497556	29.740698313503355	30.708533933659467	19.46290148933963
36	24.008641594602523	29.965964208031526	27.85252138051556	18.17287281685039
37	20.28897216646916	32.798068062250216	29.014642103649596	17.89831766763103
38	21.352981066536188	32.15536920810871	27.12484971803172	19.366800007323384
39	21.011689774220752	31.58594010547829	28.278641885383994	19.123728234916967
40	22.25982437516985	29.34889720228734	28.40249325369032	19.988785168852495
41	19.007990028708388	28.879697960616703	29.68010089040159	22.432211120273323
42	20.50048876416797	29.51345797687174	27.86813742260636	22.11791583635393
43	20.642756292457236	29.395458136981052	28.48990719045145	21.471878380110258
44	20.67621411137361	30.70322088945386	27.72425444168701	20.896310557485524
45	19.495820824045975	33.00965645784369	26.398183226073265	21.096339492037075
46	21.277665075027006	32.11889587761618	27.020563276023058	19.582875771333757
47	20.26395060017655	30.509510169633298	28.431463704189802	20.79507552600035
48	20.58531797672097	29.65486393042497	30.14506405628668	19.614754036567387
49	21.129007534112077	28.69205416289578	29.475405092696466	20.703533210295674
50	20.118416267679784	31.329370328873846	28.332310811650068	20.219902591796302
51	18.778523957342	31.8556488968074	27.515825153459023	21.850001992391576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2293.0
1	3060.5
2	3828.0
3	36875.0
4	69922.0
5	46168.0
6	22414.0
7	23473.5
8	24533.0
9	28432.5
10	32332.0
11	35239.0
12	38146.0
13	38179.5
14	38213.0
15	35595.0
16	32977.0
17	30110.0
18	27243.0
19	26006.5
20	24770.0
21	22823.5
22	20877.0
23	21206.0
24	21535.0
25	22621.0
26	26286.0
27	28865.0
28	32844.5
29	36824.0
30	44396.0
31	51968.0
32	58678.5
33	65389.0
34	71984.5
35	78580.0
36	84302.5
37	90025.0
38	101074.0
39	112123.0
40	130472.0
41	148821.0
42	170403.5
43	191986.0
44	196749.5
45	201513.0
46	207536.0
47	213559.0
48	213617.0
49	213675.0
50	201748.0
51	189821.0
52	179439.0
53	169057.0
54	155621.0
55	142185.0
56	130901.0
57	119617.0
58	109424.0
59	99231.0
60	88545.5
61	77860.0
62	69698.5
63	61537.0
64	51318.5
65	41100.0
66	33329.5
67	25559.0
68	20869.0
69	16179.0
70	13392.5
71	10606.0
72	8350.5
73	6095.0
74	5422.0
75	3880.0
76	3011.0
77	2227.0
78	1443.0
79	1134.0
80	825.0
81	582.5
82	340.0
83	223.5
84	107.0
85	96.0
86	85.0
87	65.5
88	46.0
89	29.5
90	13.0
91	10.0
92	7.0
93	6.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2785597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.760403577335865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.27929330626358	18.271891145538767
2	7.39057890001676	3.3642511686904886
3	2.669773486834888	1.822953660612997
4	1.4225727624560514	1.2951332076650108
5	0.9864650214291226	1.122617100132605
6	0.7264358481497022	0.9920383846158898
7	0.5444104023661154	0.8673700328686812
8	0.4134515582548089	0.7528259460446272
9	0.31035131690099516	0.6357349101081875
>10	3.184224848179644	16.78049076527382
>50	1.0970541569953023	18.183895410055325
>100	0.9714059492909478	33.30215757052803
>500	0.0030266565751605025	0.4473726885844648
>1k	7.964885724106585E-4	0.29697947096161365
>5k	0.0	0.0
>10k+	1.5929771448213172E-4	1.8642885383195227
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51419	1.8458879730269668	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2944	0.1056865009547325	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.589894733516729E-5	0.0	0.0	0.04756610521909666	0.0
2	3.589894733516729E-5	0.0	0.0	0.17310472405017668	0.0
3	3.589894733516729E-5	0.0	0.0	0.2524413976608964	0.0
4	3.589894733516729E-5	0.0	0.0	0.3822160922775261	0.0
5	3.589894733516729E-5	0.0	0.0	0.6428065509835055	0.0
6	3.589894733516729E-5	0.0	0.0	0.9864312748757268	0.0
7	3.589894733516729E-5	0.0	0.0	1.1659619104988985	0.0
8	3.589894733516729E-5	0.0	0.0	1.4769544912634527	0.0
9	3.589894733516729E-5	0.0	0.0	1.6054368237760164	0.0
10	3.589894733516729E-5	0.0	0.0	1.8520625919686158	0.0
11	3.589894733516729E-5	0.0	0.0	2.1904101706025676	0.0
12	3.589894733516729E-5	0.0	0.0	2.4220301788090666	0.0
13	3.589894733516729E-5	0.0	0.0	2.53862995975369	0.0
14	3.589894733516729E-5	0.0	0.0	2.592729673387787	0.0
15	3.589894733516729E-5	0.0	0.0	2.6623736312180117	0.0
16	3.589894733516729E-5	0.0	0.0	2.8144415721297804	0.0
17	3.589894733516729E-5	0.0	0.0	2.9899156267040783	0.0
18	3.589894733516729E-5	0.0	0.0	3.1764465570576075	0.0
19	3.589894733516729E-5	0.0	0.0	3.2862973359032193	0.0
20	3.589894733516729E-5	0.0	0.0	3.4007072810603973	0.0
21	3.589894733516729E-5	0.0	0.0	3.5612114745959302	0.0
22	3.589894733516729E-5	0.0	0.0	3.7322699586480024	0.0
23	3.589894733516729E-5	0.0	0.0	3.9274884342566425	0.0
24	3.589894733516729E-5	0.0	0.0	4.07614597517157	0.0
25	3.589894733516729E-5	0.0	0.0	4.212920964518557	0.0
26	3.589894733516729E-5	0.0	0.0	4.332679852828676	0.0
27	3.589894733516729E-5	0.0	0.0	4.462598143234646	0.0
28	3.589894733516729E-5	0.0	0.0	4.590829183115863	0.0
29	3.589894733516729E-5	0.0	0.0	4.732953115615791	0.0
30	3.589894733516729E-5	0.0	0.0	4.897513890200198	0.0
31	7.179789467033458E-5	0.0	0.0	5.041217376382872	0.0
32	7.179789467033458E-5	0.0	0.0	5.179715515201948	0.0
33	7.179789467033458E-5	0.0	0.0	5.3229882140166005	0.0
34	7.179789467033458E-5	0.0	0.0	5.469061030723396	0.0
35	7.179789467033458E-5	0.0	0.0	5.63304742215044	0.0
36	7.179789467033458E-5	0.0	0.0	5.782817830432759	0.0
37	1.0769684200550187E-4	0.0	0.0	5.937865383973346	0.0
38	1.0769684200550187E-4	0.0	0.0	6.103718520661818	0.0
39	1.0769684200550187E-4	0.0	0.0	6.304860322580761	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTCG	20	7.0350175E-4	45.000004	11
CGTTTTT	23130	0.0	43.6965	1
CGTACAA	65	0.0	41.538464	37
TACGCGG	220	0.0	39.886364	2
GCGATAC	170	0.0	39.705883	9
CGCTATC	40	3.460591E-7	39.375004	44
ATAGGGT	1670	0.0	39.341316	4
GGCGATA	1130	0.0	39.225662	8
TCGATAG	75	0.0	39.000004	1
TACGGGA	595	0.0	38.94958	4
GGGCGAT	4420	0.0	38.78959	7
GGTACGC	35	6.251492E-6	38.57143	8
CGTATAG	70	0.0	38.57143	14
GTTTTTT	26865	0.0	38.525963	2
TATTGCG	235	0.0	38.29787	1
TAGGGCG	1165	0.0	38.04721	5
CACGACG	185	0.0	37.7027	26
ATTGGGC	1155	0.0	37.5974	4
TGGGCGA	1450	0.0	37.551723	6
CGCGAAC	30	1.140339E-4	37.500004	44
>>END_MODULE