FastQCFastQC Report
Thu 26 May 2016
SRR1547409_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547409_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2602681
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT808443.1061816642147075No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG600.045.0000041
TTACGCG302.1665637E-645.0000041
ACGACCG351.2127202E-745.00000429
CGCGAAA207.034933E-445.010
CGTTTTT410050.044.2263151
CGTAAGG3650.043.767122
CGCACGG650.041.538462
GCGATAT1700.039.7058839
GATCGAC403.4604636E-739.3759
TGCGTCC403.4604636E-739.37521
GTTTTTT463300.039.356792
GACCGAT11200.038.9732179
TAGGGAC23950.038.611695
TATAGCG1400.038.571431
AGGGCGA17350.038.5158546
GGGCGAT33950.038.240067
CGACGGT1250.037.80000328
TCGTAAG1250.037.8000031
GGCGATA7800.037.788468
CGAGGGA15300.037.647064
Produced by FastQC (version 0.11.5)