FastQCFastQC Report
Thu 26 May 2016
SRR1547408_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547408_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2956767
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT736172.489780222790636No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA35480.11999592798485642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT393000.043.9351121
TACGGGA11850.040.063294
GACCGAT15000.039.39
GTTTTTT451650.038.6433072
TAGGGAC31500.038.571435
TAGCACG1000.038.251
CGAGGGA19400.038.0412374
CGGGTAT3500.037.928576
TAGGGAT46600.037.902365
TAAGGGA41150.037.7278254
TACGGGT3350.037.6119424
CGTAAGG3700.037.0945972
CACGACC8500.037.05882627
ATAGGGA45500.036.939564
ATAACGC1100.036.8181811
TTACGGG7350.036.7346953
GGACCGA17350.036.7002878
GTAGGGA39800.036.5766334
TATGGGA33900.036.5044254
GCGTAAG1850.036.486491
Produced by FastQC (version 0.11.5)