##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547408_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2956767
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332613290123977	31.0	31.0	33.0	30.0	34.0
2	31.72977512262549	31.0	31.0	34.0	30.0	34.0
3	31.632000762995528	31.0	31.0	34.0	30.0	34.0
4	35.52185444439822	37.0	35.0	37.0	33.0	37.0
5	35.47444658304155	37.0	35.0	37.0	33.0	37.0
6	35.53384930229538	37.0	35.0	37.0	33.0	37.0
7	35.94844436507848	37.0	35.0	37.0	35.0	37.0
8	35.93998005253711	37.0	35.0	37.0	35.0	37.0
9	37.66302789499477	39.0	37.0	39.0	35.0	39.0
10	37.018425868524645	39.0	37.0	39.0	33.0	39.0
11	36.73618617902594	39.0	37.0	39.0	32.0	39.0
12	36.51313715284295	39.0	35.0	39.0	32.0	39.0
13	36.44054908621477	39.0	35.0	39.0	32.0	39.0
14	37.5867367973195	40.0	36.0	41.0	32.0	41.0
15	37.66159592554976	40.0	36.0	41.0	32.0	41.0
16	37.69394916812857	40.0	36.0	41.0	32.0	41.0
17	37.589203342705055	40.0	36.0	41.0	32.0	41.0
18	37.32662972767215	39.0	36.0	40.0	32.0	41.0
19	37.11595198404203	38.0	36.0	40.0	32.0	41.0
20	36.81615257475479	38.0	35.0	40.0	32.0	41.0
21	36.68714511491775	38.0	35.0	40.0	31.0	41.0
22	36.62763315472609	38.0	35.0	40.0	31.0	41.0
23	36.51677964479447	38.0	35.0	40.0	31.0	41.0
24	36.344671730981844	38.0	35.0	40.0	31.0	41.0
25	36.265116595254206	38.0	35.0	40.0	31.0	41.0
26	36.13086726143791	38.0	35.0	40.0	30.0	41.0
27	36.05498945300729	38.0	35.0	40.0	30.0	41.0
28	36.05429139326839	38.0	35.0	40.0	30.0	41.0
29	36.0236535377999	38.0	35.0	40.0	30.0	41.0
30	35.900845078425185	38.0	35.0	40.0	30.0	41.0
31	35.71225734053444	38.0	35.0	40.0	29.0	41.0
32	35.3981243703004	38.0	34.0	40.0	27.0	41.0
33	34.89599789229249	38.0	34.0	40.0	24.0	41.0
34	34.297322379477315	38.0	33.0	40.0	21.0	41.0
35	33.85395568876411	38.0	33.0	40.0	18.0	41.0
36	33.573103663562264	37.0	33.0	40.0	16.0	41.0
37	33.43698235268454	37.0	33.0	40.0	15.0	41.0
38	33.323300415622874	37.0	32.0	40.0	15.0	41.0
39	33.26144163540786	37.0	32.0	40.0	15.0	41.0
40	33.129552988111676	37.0	32.0	40.0	15.0	41.0
41	33.02142678134598	37.0	32.0	40.0	14.0	41.0
42	32.9369104836465	37.0	32.0	40.0	13.0	41.0
43	32.87007633675565	37.0	32.0	40.0	12.0	41.0
44	32.72984716076715	36.0	31.0	40.0	12.0	41.0
45	32.64089459872895	36.0	31.0	40.0	11.0	41.0
46	32.5515027054888	36.0	31.0	40.0	11.0	41.0
47	32.41679645369419	36.0	31.0	40.0	10.0	41.0
48	32.37151523944903	35.0	31.0	40.0	10.0	41.0
49	32.23410806465305	35.0	31.0	40.0	10.0	41.0
50	32.179129096070135	35.0	31.0	40.0	10.0	41.0
51	31.771770315347812	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	3.0
12	3.0
13	16.0
14	39.0
15	126.0
16	388.0
17	903.0
18	2211.0
19	4175.0
20	7014.0
21	11071.0
22	16852.0
23	24616.0
24	37443.0
25	58380.0
26	85552.0
27	96512.0
28	90276.0
29	85201.0
30	87026.0
31	96669.0
32	112220.0
33	138314.0
34	199004.0
35	251526.0
36	244952.0
37	287023.0
38	404328.0
39	614749.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.570033756464408	22.178548394242767	24.914239099665274	23.337178749627547
2	39.10017258715347	23.247418548705394	25.73418872707927	11.918220137061866
3	25.72153977638414	23.457884912811867	38.89904074281132	11.921534567992676
4	22.19082531697628	25.50231384481767	38.893798530624835	13.41306230758122
5	20.393862620896407	29.66987253307413	37.45678979777575	12.479475048253716
6	19.837477893929417	37.02760481295956	33.90162295507221	9.23329433803881
7	80.63824440681326	2.4581240253290164	15.628996129894576	1.2746354379631537
8	81.058737465617	2.14037832538039	15.650810496735115	1.1500737122674867
9	76.63776009404867	4.74217278534291	16.643414919065318	1.9766522015431043
10	34.61317716275919	32.975510075700925	22.793037124670292	9.618275636869594
11	25.27402395927714	24.540959771263683	34.153722630156516	16.031293639302657
12	24.683345018393403	21.365532015204444	35.84976428646559	18.101358679936567
13	22.008700719400615	23.949435312285345	36.125707571817465	17.91615639649658
14	18.62399032456734	25.358203740774975	37.072721658487126	18.945084276170558
15	18.15391608469656	25.30405676199714	39.47307988759344	17.068947265712854
16	21.99540917495359	24.023840904609663	37.63235317493736	16.34839674549939
17	20.81371308594827	24.750039485695016	35.63165443878398	18.804592989572733
18	21.209550837113646	24.509675601763682	36.96534086047362	17.31543270064905
19	22.429396702547074	26.542267280445163	33.75524686253601	17.27308915447176
20	23.40759349654538	26.11331904069546	35.04750289759051	15.431584565168647
21	23.12380380327567	25.629310662625766	35.84303396243262	15.403851571665944
22	23.26645961619566	22.91797087832758	35.525998497683446	18.28957100779331
23	20.360143359283974	25.907486115747368	35.732541657831	17.999828867137655
24	19.61821814163916	25.664010725227925	37.69928438730546	17.018486745827452
25	19.99142982859319	27.0505927589154	35.0984030868851	17.85957432560631
26	19.589369064251596	27.611712387212112	34.217982005345704	18.580936543190585
27	18.268399234704663	27.277394532609435	35.67250987311479	18.78169635957111
28	17.795720799102533	27.32538613965862	36.534667763810944	18.3442252974279
29	19.12118878491271	25.721877983621976	36.372700317610416	18.784232913854897
30	19.473634547463497	26.025791007543038	36.44994685073257	18.05062759426089
31	21.07633100612933	26.24092463153167	34.40944112268569	18.27330323965331
32	21.737154128140634	25.67111307722252	34.30513801053651	18.286594784100338
33	21.285512182732017	26.79757992428893	33.24387751892523	18.673030374053823
34	19.793815339524556	27.77699426434345	34.05648804927815	18.372702346853846
35	20.296458936399116	28.824286797032027	32.51717162698312	18.362082639585736
36	21.506327688316325	29.92055173775952	29.976660318516814	18.59646025540734
37	20.613020911015308	30.04765001773897	30.5505641804038	18.78876489084192
38	21.13500996189419	29.973785556995193	30.24529832753139	18.64590615357923
39	20.737819381777463	29.5794359176763	29.92521899764168	19.75752570290456
40	22.438156270007074	28.34721166733801	30.059825478301132	19.15480658435379
41	19.754549479211587	28.748257809966088	30.690480514697306	20.806712196125023
42	20.650561914415306	28.456790812397458	29.89643756170168	20.99620971148555
43	20.30082180976722	28.90745195681635	29.94615402566384	20.84557220775259
44	20.26906415013425	28.9980576758331	29.904486893962222	20.82839128007043
45	20.17264126662669	29.459270886072524	29.190125566201193	21.177962281099592
46	20.379252068221813	29.817770558180605	29.363896445002265	20.43908092859532
47	19.76801012727753	29.41547304877253	30.987189724452417	19.829327099497526
48	20.326593201290464	28.614429205953666	31.123385779129702	19.935591813626168
49	20.64444036341044	28.493317194083943	30.685238302510815	20.177004139994796
50	19.852494295289418	28.747378471147712	31.313052398109154	20.087074835453723
51	19.117028835887307	28.905388892665535	30.86976417147513	21.107818099972032
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2193.0
1	3098.5
2	4004.0
3	57766.5
4	111529.0
5	76193.0
6	40857.0
7	40394.0
8	39931.0
9	41568.5
10	43206.0
11	43930.0
12	44654.0
13	43730.0
14	42806.0
15	40994.0
16	39182.0
17	36451.0
18	33720.0
19	31686.0
20	29652.0
21	28988.5
22	28325.0
23	27685.5
24	27046.0
25	28850.0
26	34377.5
27	38101.0
28	42308.5
29	46516.0
30	54140.0
31	61764.0
32	69456.5
33	77149.0
34	86114.5
35	95080.0
36	101251.0
37	107422.0
38	114843.5
39	122265.0
40	131716.0
41	141167.0
42	149958.5
43	158750.0
44	165796.0
45	172842.0
46	178609.0
47	184376.0
48	183082.0
49	181788.0
50	179405.0
51	177022.0
52	166767.5
53	156513.0
54	149367.5
55	142222.0
56	134476.0
57	126730.0
58	119450.0
59	112170.0
60	103566.5
61	94963.0
62	86262.5
63	77562.0
64	68075.0
65	58588.0
66	48788.5
67	38989.0
68	32128.0
69	25267.0
70	20599.0
71	15931.0
72	13087.0
73	10243.0
74	8715.5
75	5702.5
76	4217.0
77	3307.0
78	2397.0
79	1670.0
80	943.0
81	668.5
82	394.0
83	320.5
84	247.0
85	184.5
86	122.0
87	80.0
88	38.0
89	26.0
90	14.0
91	12.0
92	10.0
93	9.5
94	9.0
95	8.0
96	7.0
97	4.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2956767.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.363320735650902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.36158301856881	21.44233129490433
2	6.565239807702991	3.5929352512928006
3	2.812061961946711	2.308420601798148
4	1.7466949705211596	1.9118149882287514
5	1.2110232155314709	1.6568808332453464
6	0.8773358241400724	1.4404092929292849
7	0.65948930691508	1.2632072198794617
8	0.55812172597078	1.221765103781881
9	0.4413147810643601	1.0868254109682878
>10	5.517521813741677	35.87592183610066
>50	1.146212798174099	20.95191735840958
>100	0.10001149361367684	3.8243600466561523
>500	0.0022595214060531356	0.4187130161188671
>1k	0.0010042317360236125	0.47583522660333477
>5k	0.0	0.0
>10k+	1.2552896700295156E-4	2.5286625190830647
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73617	2.489780222790636	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	3548	0.11999592798485642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.382072378378141E-5	0.0	0.0	0.026075778037295466	0.0
2	3.382072378378141E-5	0.0	0.0	0.06625479789242777	0.0
3	3.382072378378141E-5	0.0	0.0	0.10690730788053303	0.0
4	3.382072378378141E-5	0.0	0.0	0.15401957611134054	0.0
5	3.382072378378141E-5	0.0	0.0	0.2877128972286284	0.0
6	3.382072378378141E-5	0.0	0.0	0.5767786234086081	0.0
7	3.382072378378141E-5	0.0	0.0	0.7526463870842715	0.0
8	3.382072378378141E-5	0.0	0.0	1.0193566148431716	0.0
9	3.382072378378141E-5	0.0	3.382072378378141E-5	1.2033751729507263	0.0
10	3.382072378378141E-5	0.0	3.382072378378141E-5	1.4188808249009814	0.0
11	6.764144756756282E-5	0.0	3.382072378378141E-5	1.6177805014734	0.0
12	6.764144756756282E-5	0.0	3.382072378378141E-5	1.7659152716463624	0.0
13	6.764144756756282E-5	0.0	3.382072378378141E-5	1.8686626305014904	0.0
14	6.764144756756282E-5	0.0	3.382072378378141E-5	1.9344777589847288	0.0
15	6.764144756756282E-5	0.0	3.382072378378141E-5	1.9901466703328332	0.0
16	6.764144756756282E-5	0.0	3.382072378378141E-5	2.05934387119445	0.0
17	6.764144756756282E-5	0.0	3.382072378378141E-5	2.1389578549814714	0.0
18	6.764144756756282E-5	0.0	3.382072378378141E-5	2.2340617302614647	0.0
19	6.764144756756282E-5	0.0	3.382072378378141E-5	2.3019399228955137	0.0
20	1.0146217135134422E-4	0.0	3.382072378378141E-5	2.3756691007441573	0.0
21	1.0146217135134422E-4	0.0	3.382072378378141E-5	2.4632309546203675	0.0
22	1.0146217135134422E-4	0.0	3.382072378378141E-5	2.5633064762965767	0.0
23	1.0146217135134422E-4	0.0	3.382072378378141E-5	2.68255834835819	0.0
24	1.0146217135134422E-4	0.0	3.382072378378141E-5	2.773468453888994	0.0
25	1.0146217135134422E-4	0.0	3.382072378378141E-5	2.8647505873814203	0.0
26	1.0146217135134422E-4	0.0	3.382072378378141E-5	2.950012632040333	0.0
27	1.0146217135134422E-4	0.0	3.382072378378141E-5	3.0405168888857323	0.0
28	1.3528289513512563E-4	0.0	3.382072378378141E-5	3.1360604335749147	0.0
29	1.3528289513512563E-4	0.0	3.382072378378141E-5	3.2447602398159883	0.0
30	1.3528289513512563E-4	0.0	3.382072378378141E-5	3.3719261612430063	0.0
31	1.3528289513512563E-4	0.0	3.382072378378141E-5	3.4856652553278633	0.0
32	1.3528289513512563E-4	0.0	3.382072378378141E-5	3.6108357540516383	0.0
33	1.3528289513512563E-4	0.0	3.382072378378141E-5	3.7310007856554135	0.0
34	1.3528289513512563E-4	0.0	3.382072378378141E-5	3.847411716919189	0.0
35	1.3528289513512563E-4	0.0	3.382072378378141E-5	3.975998108745126	0.0
36	1.3528289513512563E-4	0.0	3.382072378378141E-5	4.098090921604577	0.0
37	1.3528289513512563E-4	0.0	3.382072378378141E-5	4.227387548629973	0.0
38	1.3528289513512563E-4	0.0	3.382072378378141E-5	4.37636783689753	0.0
39	1.3528289513512563E-4	0.0	3.382072378378141E-5	4.578581944400759	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	39300	0.0	43.935112	1
TACGGGA	1185	0.0	40.06329	4
GACCGAT	1500	0.0	39.3	9
GTTTTTT	45165	0.0	38.643307	2
TAGGGAC	3150	0.0	38.57143	5
TAGCACG	100	0.0	38.25	1
CGAGGGA	1940	0.0	38.041237	4
CGGGTAT	350	0.0	37.92857	6
TAGGGAT	4660	0.0	37.90236	5
TAAGGGA	4115	0.0	37.727825	4
TACGGGT	335	0.0	37.611942	4
CGTAAGG	370	0.0	37.094597	2
CACGACC	850	0.0	37.058826	27
ATAGGGA	4550	0.0	36.93956	4
ATAACGC	110	0.0	36.81818	11
TTACGGG	735	0.0	36.734695	3
GGACCGA	1735	0.0	36.700287	8
GTAGGGA	3980	0.0	36.576633	4
TATGGGA	3390	0.0	36.504425	4
GCGTAAG	185	0.0	36.48649	1
>>END_MODULE