FastQCFastQC Report
Thu 26 May 2016
SRR1547406_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547406_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4051921
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810612.0005572665409814No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT454350.044.044241
TACGGGA10800.040.6254
AGGGCGA35300.039.9008526
CTTAACG1250.039.61
GTTTTTT514050.039.3449062
GGCGATA13650.038.7362638
GGGCGAT62550.038.7050367
TAGGGTC19700.038.3756335
TAGGGTG30400.038.190795
ACGGGTA8350.037.994015
ACGGGAT13750.037.6363645
GCGCGAC3950.037.5949369
TGGGCGA18800.037.579796
CGTTCGC301.1404308E-437.49999621
CCGTCGA301.1404308E-437.49999610
GGCGATT18100.037.417138
AAGGGCG15050.037.2259145
CGACCCG4050.037.2222232
CGGGTAT7400.037.0945936
CGTAAGG4800.037.0312462
Produced by FastQC (version 0.11.5)