##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547406_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4051921
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.293440074473317	31.0	31.0	33.0	30.0	34.0
2	31.6919029270314	31.0	31.0	34.0	30.0	34.0
3	31.668055966540315	31.0	31.0	34.0	30.0	34.0
4	35.535558319128135	37.0	35.0	37.0	33.0	37.0
5	35.469944009273625	37.0	35.0	37.0	33.0	37.0
6	35.52220341906962	37.0	35.0	37.0	33.0	37.0
7	35.94065481533327	37.0	35.0	37.0	35.0	37.0
8	35.932057905373775	37.0	35.0	37.0	35.0	37.0
9	37.66809742835559	39.0	37.0	39.0	35.0	39.0
10	36.86237293372699	39.0	37.0	39.0	32.0	39.0
11	36.65615889352236	39.0	35.0	39.0	32.0	39.0
12	36.43803247891556	39.0	35.0	39.0	32.0	39.0
13	36.32318991411728	39.0	35.0	39.0	32.0	39.0
14	37.45713033398233	40.0	36.0	41.0	32.0	41.0
15	37.57055554636924	40.0	36.0	41.0	32.0	41.0
16	37.62317256432196	40.0	36.0	41.0	32.0	41.0
17	37.54387486824151	39.0	36.0	41.0	32.0	41.0
18	37.32877294498091	39.0	36.0	41.0	32.0	41.0
19	37.15444007916245	38.0	36.0	41.0	32.0	41.0
20	36.88749928737505	38.0	35.0	41.0	32.0	41.0
21	36.77836315170014	38.0	35.0	40.0	31.0	41.0
22	36.69978067193314	38.0	35.0	40.0	31.0	41.0
23	36.598616063837376	38.0	35.0	40.0	31.0	41.0
24	36.419467704330856	38.0	35.0	40.0	31.0	41.0
25	36.31414630245752	38.0	35.0	40.0	31.0	41.0
26	36.16512266650806	38.0	35.0	40.0	30.0	41.0
27	36.08099146059363	38.0	35.0	40.0	30.0	41.0
28	36.08791953248842	38.0	35.0	40.0	30.0	41.0
29	36.05651393499528	38.0	35.0	40.0	30.0	41.0
30	35.932501398719275	38.0	35.0	40.0	30.0	41.0
31	35.759904746415344	38.0	35.0	40.0	29.0	41.0
32	35.5018353023171	38.0	34.0	40.0	27.0	41.0
33	35.0928265876852	38.0	34.0	40.0	25.0	41.0
34	34.643213182093135	38.0	34.0	40.0	23.0	41.0
35	34.31031429289959	38.0	33.0	40.0	21.0	41.0
36	34.06245136566088	38.0	33.0	40.0	18.0	41.0
37	33.930963116013366	38.0	33.0	40.0	18.0	41.0
38	33.81527132439157	37.0	33.0	40.0	18.0	41.0
39	33.75221259249625	37.0	33.0	40.0	18.0	41.0
40	33.593288220574884	37.0	33.0	40.0	17.0	41.0
41	33.480129548429005	37.0	33.0	40.0	15.0	41.0
42	33.38915590901205	37.0	33.0	40.0	15.0	41.0
43	33.314819316566144	37.0	33.0	40.0	15.0	41.0
44	33.17427091989207	37.0	32.0	40.0	15.0	41.0
45	33.07440470828528	36.0	32.0	40.0	15.0	41.0
46	32.985345963062954	36.0	32.0	40.0	15.0	41.0
47	32.84318573832017	36.0	31.0	40.0	14.0	41.0
48	32.77923533059998	36.0	31.0	40.0	13.0	41.0
49	32.64849413401693	36.0	31.0	40.0	13.0	41.0
50	32.56531260110945	36.0	31.0	40.0	12.0	41.0
51	32.16283288840034	35.0	31.0	39.0	11.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	7.0
12	3.0
13	18.0
14	56.0
15	187.0
16	454.0
17	1148.0
18	2606.0
19	5262.0
20	8897.0
21	14196.0
22	21821.0
23	32919.0
24	49520.0
25	75434.0
26	105657.0
27	115267.0
28	109701.0
29	107711.0
30	114774.0
31	130602.0
32	154665.0
33	191699.0
34	280005.0
35	378798.0
36	338434.0
37	387114.0
38	553378.0
39	871341.0
40	244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.24099458010164	23.282462812083455	26.889887537294037	20.58665507052087
2	35.81019965591629	24.451439206243162	27.69646792225219	12.04189321558836
3	25.92271665711153	23.62792858004882	38.05878742453271	12.390567338306942
4	22.404879068471473	26.265368944754847	37.853279962763345	13.476472024010338
5	20.923853155083723	30.136668508591356	36.37097070747431	12.568507628850611
6	19.434090644906455	38.47449148194153	32.68425026055542	9.407167612596593
7	82.86792857017696	2.7171803201493807	13.33436658809488	1.080524521578777
8	83.5059469323316	2.205373698055811	12.926239183834037	1.362440185778548
9	79.38511140765084	4.1779442392879815	14.591079144929035	1.8458652081321427
10	34.35039824320366	25.775082979159762	27.868016182941375	12.006502594695208
11	27.84358332751305	25.32825788064476	30.696723850242886	16.131434941599306
12	27.170717296808107	23.00923438536931	31.836331458584706	17.983716859237877
13	21.889814732320794	27.257540312360483	32.89262056195074	17.960024393367984
14	18.527138115476586	29.440578925403532	33.66682124355337	18.365461715566518
15	18.26467001701168	27.116545460782675	38.05335790110419	16.565426621101448
16	19.924179173285957	25.46086165056031	37.5626030221221	17.05235615403163
17	19.87084644542675	26.255052850240663	33.42634271497396	20.447757989358628
18	20.61891137561665	26.54489068271568	35.29506128080977	17.5411366608579
19	22.530424457929954	28.723462278756173	31.118276985163334	17.627836278150536
20	23.8704555197399	27.660509669364234	32.20963587394719	16.25939893694867
21	21.88278103151567	28.336386617606806	33.32093592150488	16.459896429372638
22	21.65883786974129	25.75933736121706	33.04595523950245	19.535869529539198
23	19.812972661609148	28.420124676665708	32.79400067276731	18.97290198895783
24	18.94000401291141	27.298656612505525	36.931815797988165	16.829523576594905
25	19.153137487132646	28.952267331964272	33.79577242498065	18.098822755922438
26	18.834918054917654	31.00307236987098	31.580230710322336	18.581778864889024
27	18.109977958602848	30.869678851093095	32.65399300726742	18.36635018303664
28	17.089523709865023	29.68814051409196	35.34402571027421	17.878310065768805
29	18.363339265499008	27.160722037769247	34.77836808763053	19.697570609101213
30	19.12569371416669	29.736142437130436	33.56647377873359	17.571690069969282
31	20.857859765775295	29.049480480986674	31.48849644403235	18.60416330920568
32	22.138264788479344	28.26049668786731	31.115809019968555	18.485429503684795
33	20.6740210384161	29.5258471228832	30.64124399266422	19.158887846036485
34	18.515415280801378	30.110434038570844	31.001344794234637	20.372805886393145
35	18.99496559779917	29.446378643611265	31.29268808547847	20.2659676731111
36	21.17524008982406	30.711334204195985	28.698165635509675	19.41526007047028
37	19.064932411071194	31.828705446132833	29.718422447031912	19.387939695764057
38	19.352549074870907	31.697162901251037	29.34630265496292	19.60398536891514
39	19.093486768374802	31.79489432296434	28.89774998081157	20.213868927849283
40	21.299205981557883	30.222627736325556	28.750535856942918	19.72763042517364
41	18.541279556042678	29.06411551459172	30.42021302981968	21.97439189954592
42	19.814009206990956	29.94379702861926	28.543942490487844	21.698251273901935
43	19.746806514737084	30.532949679917255	28.5753596874174	21.14488411792826
44	19.697990163184325	31.005046742026803	28.346529954557358	20.95043314023151
45	19.056911524188155	32.391821064625894	27.041593357817195	21.50967405336876
46	20.28203412652912	32.20931503847188	27.15593418529137	20.352716649707634
47	19.706085089023205	30.347556134485348	29.208071924403267	20.738286852088184
48	19.90357166390954	29.407483512141525	30.27852714798734	20.4104176759616
49	19.74256161460206	29.44033212888405	29.634462271105484	21.182643985408404
50	19.110910602649952	30.825625672366268	30.026695979511942	20.03676774547184
51	17.827025749021267	31.439309897700372	28.935263051772232	21.798401301506125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3036.0
1	3841.0
2	4646.0
3	59032.5
4	113419.0
5	77509.0
6	41599.0
7	42827.5
8	44056.0
9	48955.5
10	53855.0
11	57089.5
12	60324.0
13	60812.0
14	61300.0
15	58609.0
16	55918.0
17	51713.5
18	47509.0
19	43893.0
20	40277.0
21	38482.0
22	36687.0
23	36269.0
24	35851.0
25	38079.5
26	43512.5
27	46717.0
28	53749.0
29	60781.0
30	67605.5
31	74430.0
32	83647.0
33	92864.0
34	108089.5
35	123315.0
36	132388.5
37	141462.0
38	157059.5
39	172657.0
40	194525.5
41	216394.0
42	243332.0
43	270270.0
44	279258.0
45	288246.0
46	293170.5
47	298095.0
48	301164.0
49	304233.0
50	294124.0
51	284015.0
52	262740.0
53	241465.0
54	221124.0
55	200783.0
56	184809.0
57	168835.0
58	151321.0
59	133807.0
60	118994.5
61	104182.0
62	89390.5
63	74599.0
64	61035.5
65	47472.0
66	36761.0
67	26050.0
68	21486.0
69	16922.0
70	13262.5
71	9603.0
72	7850.0
73	6097.0
74	5159.5
75	3373.0
76	2524.0
77	2033.5
78	1543.0
79	1124.5
80	706.0
81	585.5
82	465.0
83	333.0
84	201.0
85	142.0
86	83.0
87	63.0
88	43.0
89	35.5
90	28.0
91	20.5
92	13.0
93	11.5
94	10.0
95	7.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4051921.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.006601397793435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.32920537162966	19.284312139582003
2	7.06337200036765	3.3913511227432207
3	2.6082817617448026	1.8784794176202566
4	1.471427750927956	1.4129591800871648
5	0.9894718721362932	1.1876928414352208
6	0.7351217882532627	1.058866544965751
7	0.591980173137583	0.994800243633749
8	0.4659748676684956	0.8949178327605692
9	0.36447348055785495	0.7874792611036954
>10	3.5044713121281212	19.108599570285357
>50	1.0651271708990153	18.310823124182217
>100	0.8066879624086433	28.676698506756672
>500	0.0030411941924163603	0.48314160930382705
>1k	0.0012584251830683269	0.48913336080251524
>5k	0.0	0.0
>10k+	1.0486876525569391E-4	2.0407452447379013
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81061	2.0005572665409814	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.467965194780451E-5	0.0	0.0	0.03361368595290974	0.0
2	2.467965194780451E-5	0.0	0.0	0.09294356923543179	0.0
3	2.467965194780451E-5	0.0	0.0	0.146572452918011	0.0
4	2.467965194780451E-5	0.0	0.0	0.2641709944492995	0.0
5	2.467965194780451E-5	0.0	0.0	0.4770576721510612	0.0
6	2.467965194780451E-5	0.0	0.0	0.9505861540735864	0.0
7	2.467965194780451E-5	0.0	0.0	1.2370181945798056	0.0
8	2.467965194780451E-5	0.0	0.0	1.7140018277750233	0.0
9	2.467965194780451E-5	0.0	0.0	1.928788838676766	0.0
10	2.467965194780451E-5	0.0	0.0	2.207693585338905	0.0
11	2.467965194780451E-5	0.0	0.0	2.4652257534142445	0.0
12	2.467965194780451E-5	0.0	0.0	2.653062584388985	0.0
13	2.467965194780451E-5	0.0	0.0	2.764417173977479	0.0
14	2.467965194780451E-5	0.0	0.0	2.822710511878193	0.0
15	2.467965194780451E-5	0.0	0.0	2.875771763565973	0.0
16	2.467965194780451E-5	0.0	0.0	2.9708624625208637	0.0
17	2.467965194780451E-5	0.0	0.0	3.0868074673716492	0.0
18	2.467965194780451E-5	0.0	0.0	3.223187224035217	0.0
19	2.467965194780451E-5	0.0	0.0	3.311885893135626	0.0
20	2.467965194780451E-5	0.0	0.0	3.4095679555450364	0.0
21	2.467965194780451E-5	0.0	0.0	3.535631617694422	0.0
22	2.467965194780451E-5	0.0	0.0	3.681167525230625	0.0
23	2.467965194780451E-5	0.0	0.0	3.8486436433484266	0.0
24	2.467965194780451E-5	0.0	0.0	3.9818397249107274	0.0
25	2.467965194780451E-5	0.0	0.0	4.1049665084783244	0.0
26	2.467965194780451E-5	0.0	0.0	4.218961820825233	0.0
27	2.467965194780451E-5	0.0	0.0	4.340484427016222	0.0
28	2.467965194780451E-5	0.0	0.0	4.477061620895372	0.0
29	2.467965194780451E-5	0.0	0.0	4.626916467522442	0.0
30	2.467965194780451E-5	0.0	0.0	4.79454066355193	2.467965194780451E-5
31	2.467965194780451E-5	0.0	0.0	4.9483936137945435	2.467965194780451E-5
32	2.467965194780451E-5	0.0	0.0	5.100938542483923	2.467965194780451E-5
33	2.467965194780451E-5	0.0	0.0	5.263380011604372	2.467965194780451E-5
34	2.467965194780451E-5	0.0	0.0	5.429079194781932	2.467965194780451E-5
35	2.467965194780451E-5	0.0	0.0	5.609215974348957	2.467965194780451E-5
36	4.935930389560902E-5	0.0	0.0	5.779974486175816	2.467965194780451E-5
37	4.935930389560902E-5	0.0	0.0	5.959864469223364	2.467965194780451E-5
38	4.935930389560902E-5	0.0	0.0	6.156610654551261	2.467965194780451E-5
39	4.935930389560902E-5	0.0	0.0	6.41123062369676	2.467965194780451E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	45435	0.0	44.04424	1
TACGGGA	1080	0.0	40.625	4
AGGGCGA	3530	0.0	39.900852	6
CTTAACG	125	0.0	39.6	1
GTTTTTT	51405	0.0	39.344906	2
GGCGATA	1365	0.0	38.736263	8
GGGCGAT	6255	0.0	38.705036	7
TAGGGTC	1970	0.0	38.375633	5
TAGGGTG	3040	0.0	38.19079	5
ACGGGTA	835	0.0	37.99401	5
ACGGGAT	1375	0.0	37.636364	5
GCGCGAC	395	0.0	37.594936	9
TGGGCGA	1880	0.0	37.57979	6
CGTTCGC	30	1.1404308E-4	37.499996	21
CCGTCGA	30	1.1404308E-4	37.499996	10
GGCGATT	1810	0.0	37.41713	8
AAGGGCG	1505	0.0	37.225914	5
CGACCCG	405	0.0	37.22222	32
CGGGTAT	740	0.0	37.094593	6
CGTAAGG	480	0.0	37.031246	2
>>END_MODULE