FastQCFastQC Report
Thu 26 May 2016
SRR1547405_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547405_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3016436
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT916133.037127258791501No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT356200.043.8882641
TACGGGA8950.039.9720654
GTTTTTT401850.039.37852
TATGGGC13750.039.2727284
AGGGCGA24100.038.8381736
TAGGGCG9000.038.5000045
GGGCGAT45350.038.4509357
GCGATAT2400.038.4375049
TTAACGG2900.038.0172422
TAAGGGA31900.037.8761754
GGCGATA11300.037.4336288
TAGGGAC24100.037.34445
TCGTCCC9650.037.0725438
TAGGGCA18450.036.4634175
AGGGATT30650.036.4110956
ATAGGGC16550.036.027194
ACGGGAT8750.036.05
TAAGGGT12950.035.9652524
GGCGATT11450.035.9606978
ATAGGGA32500.035.9307674
Produced by FastQC (version 0.11.5)