##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547405_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3016436
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255887079984458	31.0	31.0	33.0	30.0	34.0
2	31.64234646450314	31.0	31.0	34.0	30.0	34.0
3	31.58916018771822	31.0	31.0	34.0	30.0	34.0
4	35.45104023423669	37.0	35.0	37.0	33.0	37.0
5	35.38599492911502	37.0	35.0	37.0	33.0	37.0
6	35.433414798126	37.0	35.0	37.0	33.0	37.0
7	35.88784081611544	37.0	35.0	37.0	35.0	37.0
8	35.897298997890225	37.0	35.0	37.0	35.0	37.0
9	37.64950590697101	39.0	37.0	39.0	35.0	39.0
10	36.8936383864932	39.0	37.0	39.0	32.0	39.0
11	36.59559261326943	39.0	35.0	39.0	32.0	39.0
12	36.207071192626	38.0	35.0	39.0	32.0	39.0
13	36.02414902885392	38.0	35.0	39.0	31.0	39.0
14	37.109231225194236	39.0	35.0	41.0	31.0	41.0
15	37.270570965205295	39.0	35.0	41.0	32.0	41.0
16	37.37271700775352	39.0	35.0	41.0	32.0	41.0
17	37.292461699833844	39.0	35.0	41.0	32.0	41.0
18	37.05694037599339	39.0	35.0	40.0	32.0	41.0
19	36.85596379303257	38.0	35.0	40.0	31.0	41.0
20	36.585924912711555	38.0	35.0	40.0	31.0	41.0
21	36.45440977365341	38.0	35.0	40.0	31.0	41.0
22	36.361875405279605	38.0	35.0	40.0	31.0	41.0
23	36.28605049137459	38.0	35.0	40.0	31.0	41.0
24	36.13429225748532	38.0	35.0	40.0	30.0	41.0
25	36.014039084535526	37.0	35.0	40.0	30.0	41.0
26	35.770214252846735	37.0	34.0	40.0	30.0	41.0
27	35.69905113186555	37.0	34.0	40.0	30.0	41.0
28	35.66526423898932	37.0	34.0	40.0	30.0	41.0
29	35.61208326647739	37.0	34.0	40.0	29.0	41.0
30	35.5365050012664	37.0	34.0	40.0	29.0	41.0
31	35.3859551470676	37.0	34.0	40.0	28.0	41.0
32	35.08261604091716	37.0	34.0	40.0	26.0	41.0
33	34.70217733775886	37.0	33.0	40.0	24.0	41.0
34	34.281473898335655	37.0	33.0	40.0	22.0	41.0
35	33.97371069699473	36.0	33.0	40.0	21.0	41.0
36	33.6655781856469	36.0	33.0	40.0	18.0	41.0
37	33.59104287311251	36.0	33.0	40.0	18.0	41.0
38	33.48623408552344	36.0	33.0	40.0	18.0	41.0
39	33.331527670403084	36.0	33.0	40.0	18.0	41.0
40	33.19604758728513	36.0	32.0	40.0	18.0	41.0
41	33.15358721351953	36.0	32.0	40.0	17.0	41.0
42	33.05827174851381	35.0	32.0	40.0	17.0	41.0
43	32.93657249814019	35.0	32.0	40.0	16.0	41.0
44	32.82679891103275	35.0	32.0	40.0	15.0	41.0
45	32.697733020027606	35.0	31.0	40.0	15.0	41.0
46	32.61129889710904	35.0	31.0	40.0	15.0	41.0
47	32.58038857777854	35.0	31.0	40.0	15.0	41.0
48	32.44578900397688	35.0	31.0	39.0	15.0	41.0
49	32.31844434955689	35.0	31.0	39.0	15.0	41.0
50	32.18307698223997	35.0	31.0	39.0	14.0	41.0
51	31.78658224474181	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	5.0
13	11.0
14	45.0
15	144.0
16	429.0
17	1075.0
18	2426.0
19	4485.0
20	7768.0
21	11985.0
22	17696.0
23	25641.0
24	37654.0
25	55430.0
26	78167.0
27	88523.0
28	87189.0
29	87545.0
30	93309.0
31	106287.0
32	125799.0
33	155844.0
34	233076.0
35	312341.0
36	272991.0
37	284482.0
38	374410.0
39	551503.0
40	174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.844106090764065	22.540541221494507	26.859943323843105	21.755409363898323
2	37.10100264020188	23.723891373793442	26.872441517075117	12.302664468929558
3	25.81914550814272	23.588532957437188	38.0702590739535	12.522062460466591
4	22.288555102776918	26.502567931161146	37.77454585477697	13.434331111284973
5	20.64850704606363	29.96741850316068	36.55787823776138	12.826196213014299
6	20.19916882042251	37.46441164340964	33.120510430189796	9.21590910597805
7	82.1714102337991	2.5411445825470853	14.02575754963805	1.2616876340157723
8	82.64471714301249	2.0430070454005986	13.864573954163125	1.447701857423794
9	78.53718759489676	4.137995966100391	15.138428264349052	2.1863881746537968
10	38.60754877610531	26.589226491130592	22.64463757891764	12.15858715384646
11	29.944676432717287	24.189838604233604	30.27921029983729	15.586274663211816
12	28.60226439413931	21.438313294232	32.55434559195024	17.405076719678455
13	21.469641656577497	27.614774521985545	33.69761533147065	17.217968489966303
14	18.26294342064609	29.24905418182252	34.23964572760701	18.248356669924373
15	17.242202387188062	26.139556748427616	39.976880000106085	16.64136086427824
16	20.078861278674566	24.770126069308283	38.48909772990376	16.661914922113382
17	20.366651240072724	25.676195351069936	32.649789354058896	21.307364054798445
18	20.89992958577606	26.331074155062467	35.20416146737408	17.564834791787394
19	22.371401216535013	28.37003669230841	31.15968646442358	18.098875626733005
20	23.97843017388733	27.58616459954728	31.889753338045296	16.545651888520094
21	21.54867532412423	28.46445275152531	33.4010070162271	16.585864908123362
22	21.677801219717573	26.12947862974716	33.10257535714333	19.090144793391936
23	19.791237075807345	28.40255851607659	32.60622801213087	19.199976395985196
24	19.827670800905437	27.17949261976717	36.21462547191454	16.778211107412854
25	19.365668623501378	28.267995740668788	33.899078249961214	18.46725738586862
26	18.31098024290918	30.89500324223686	32.01529884937058	18.77871766548337
27	18.273817180275	30.550059739374547	33.17706724094262	17.99905583940783
28	17.38694273639487	29.41617193270469	35.15652246558522	18.040362865315227
29	17.96984918625822	27.578838072480238	35.065952004285855	19.38536073697569
30	18.58093458637942	29.22047741109044	34.08194306128159	18.11664494124855
31	21.017584991029146	28.405177500865257	32.25442210608811	18.322815402017483
32	22.355554700978242	27.65296528751149	31.933811955566107	18.057668055944166
33	21.44428060134543	28.60156820830941	31.296271493908705	18.657879696436456
34	18.417165157822012	29.421078385220174	32.25127269400047	19.910483762957345
35	18.802785804174196	30.06587907053224	31.669791767503106	19.461543357790454
36	22.37070503070511	29.754451942623678	29.103849708729108	18.7709933179421
37	19.682035355631612	32.06373349210791	29.886263126418065	18.36796802584242
38	19.978908884524653	31.841318695307976	28.72817457423264	19.45159784593474
39	19.929711752545057	31.996568135375654	28.711731327964525	19.361988784114764
40	21.90087905064122	30.203193437553455	28.273532075601803	19.62239543620352
41	18.525670692167843	29.20864225198214	29.87923496470669	22.386452091143322
42	19.699440001379113	29.758430147365967	28.75738122738225	21.784748623872677
43	19.688798303693495	29.88921362826859	29.539860948483575	20.882127119554337
44	19.259881529062774	30.703187470246345	29.230887046832756	20.80604395385813
45	18.451278263487108	32.35384407293906	28.025656768451245	21.16922089512259
46	19.894073668395418	32.00979566614375	27.96890104746131	20.12722961799952
47	19.005508487499817	30.369217182131496	29.92528268459865	20.69999164577004
48	19.960575991003953	29.298549679157787	30.60038402936446	20.140490300473804
49	19.63731370398709	29.181258942672745	30.398258076750178	20.783169276589987
50	18.48326965995632	31.09172546674287	30.430779900518363	19.994224972782447
51	18.057469145707053	31.787380869343824	28.64648877019105	21.508661214758078
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1690.0
1	2857.5
2	4025.0
3	62184.0
4	120343.0
5	76857.5
6	33372.0
7	34158.0
8	34944.0
9	39896.0
10	44848.0
11	49768.0
12	54688.0
13	55258.0
14	55828.0
15	52018.0
16	48208.0
17	42996.5
18	37785.0
19	34915.5
20	32046.0
21	29376.0
22	26706.0
23	26454.5
24	26203.0
25	27398.0
26	30700.5
27	32808.0
28	36956.5
29	41105.0
30	46826.0
31	52547.0
32	58753.5
33	64960.0
34	73520.5
35	82081.0
36	86633.5
37	91186.0
38	98785.0
39	106384.0
40	123336.0
41	140288.0
42	160117.0
43	179946.0
44	188172.0
45	196398.0
46	202392.0
47	208386.0
48	205739.5
49	203093.0
50	193039.0
51	182985.0
52	176031.0
53	169077.0
54	156336.5
55	143596.0
56	134423.5
57	125251.0
58	117040.0
59	108829.0
60	101905.5
61	94982.0
62	84709.5
63	74437.0
64	65584.5
65	56732.0
66	47883.0
67	39034.0
68	32772.5
69	26511.0
70	21925.0
71	17339.0
72	14586.0
73	11833.0
74	9883.0
75	6542.5
76	5152.0
77	3614.5
78	2077.0
79	1594.0
80	1111.0
81	906.0
82	701.0
83	463.5
84	226.0
85	167.0
86	108.0
87	72.0
88	36.0
89	28.0
90	20.0
91	12.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3016436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.005629013298474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.68191572978164	20.461727105243153
2	7.076454607802962	3.680553065199408
3	2.9052569616478046	2.266591041987466
4	1.736915748504861	1.8067834633189217
5	1.1760249008153107	1.5291633640502054
6	0.8499505317784132	1.32620989254511
7	0.604430121417306	1.1003009851428989
8	0.46394397559678296	0.965212392985979
9	0.3792009674923373	0.8875223713880621
>10	4.503865888416926	28.178979329824756
>50	1.328763744493229	24.025514742406152
>100	0.2899134345237119	9.728313187891834
>500	0.001811054930890736	0.34798414910569175
>1k	0.0014229717314141498	0.6131774058149114
>5k	0.0	0.0
>10k+	1.2936106649219544E-4	3.0819675030954894
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	91613	3.037127258791501	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.630341237142111E-5	0.0	0.0	0.05794918241262205	0.0
2	6.630341237142111E-5	0.0	0.0	0.14944789148518317	0.0
3	6.630341237142111E-5	0.0	0.0	0.22241479679993212	0.0
4	6.630341237142111E-5	0.0	0.0	0.3360588456045479	0.0
5	6.630341237142111E-5	0.0	0.0	0.6092289045748028	0.0
6	6.630341237142111E-5	0.0	0.0	1.128417775149216	0.0
7	6.630341237142111E-5	0.0	0.0	1.4837709137538473	0.0
8	6.630341237142111E-5	0.0	0.0	1.9312194921423826	0.0
9	6.630341237142111E-5	0.0	0.0	2.1842333137517254	0.0
10	6.630341237142111E-5	0.0	0.0	2.459060958031266	0.0
11	6.630341237142111E-5	0.0	0.0	2.7595480228985463	0.0
12	6.630341237142111E-5	0.0	0.0	2.9569995849406387	0.0
13	6.630341237142111E-5	0.0	0.0	3.0903357472195663	0.0
14	6.630341237142111E-5	0.0	0.0	3.1724856751477573	0.0
15	6.630341237142111E-5	0.0	0.0	3.2411760103645495	0.0
16	6.630341237142111E-5	0.0	0.0	3.340631128921681	0.0
17	6.630341237142111E-5	0.0	0.0	3.454474087963411	0.0
18	6.630341237142111E-5	0.0	0.0	3.588738498015539	0.0
19	6.630341237142111E-5	0.0	0.0	3.6842817152427565	0.0
20	6.630341237142111E-5	0.0	0.0	3.7774380096246034	0.0
21	6.630341237142111E-5	0.0	0.0	3.9014916941715323	0.0
22	6.630341237142111E-5	0.0	0.0	4.031380079007146	0.0
23	6.630341237142111E-5	0.0	0.0	4.181988280208829	0.0
24	6.630341237142111E-5	0.0	0.0	4.300605084941302	0.0
25	6.630341237142111E-5	0.0	0.0	4.410105170472703	0.0
26	6.630341237142111E-5	0.0	0.0	4.514367286426763	0.0
27	6.630341237142111E-5	0.0	0.0	4.632652574097379	0.0
28	6.630341237142111E-5	0.0	0.0	4.74795420821128	0.0
29	6.630341237142111E-5	0.0	0.0	4.874726332665437	0.0
30	6.630341237142111E-5	0.0	0.0	5.042507117671318	0.0
31	9.945511855713166E-5	0.0	0.0	5.178959540331703	0.0
32	9.945511855713166E-5	0.0	0.0	5.306958277914731	0.0
33	9.945511855713166E-5	0.0	0.0	5.441189536260673	0.0
34	9.945511855713166E-5	0.0	0.0	5.574525698539601	0.0
35	9.945511855713166E-5	0.0	0.0	5.718172041442285	0.0
36	9.945511855713166E-5	0.0	0.0	5.860724378040841	0.0
37	9.945511855713166E-5	0.0	0.0	6.009774449051795	0.0
38	9.945511855713166E-5	0.0	0.0	6.171985747418477	0.0
39	9.945511855713166E-5	0.0	0.0	6.413263865038078	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	35620	0.0	43.888264	1
TACGGGA	895	0.0	39.972065	4
GTTTTTT	40185	0.0	39.3785	2
TATGGGC	1375	0.0	39.272728	4
AGGGCGA	2410	0.0	38.838173	6
TAGGGCG	900	0.0	38.500004	5
GGGCGAT	4535	0.0	38.450935	7
GCGATAT	240	0.0	38.437504	9
TTAACGG	290	0.0	38.017242	2
TAAGGGA	3190	0.0	37.876175	4
GGCGATA	1130	0.0	37.433628	8
TAGGGAC	2410	0.0	37.3444	5
TCGTCCC	965	0.0	37.07254	38
TAGGGCA	1845	0.0	36.463417	5
AGGGATT	3065	0.0	36.411095	6
ATAGGGC	1655	0.0	36.02719	4
ACGGGAT	875	0.0	36.0	5
TAAGGGT	1295	0.0	35.965252	4
GGCGATT	1145	0.0	35.960697	8
ATAGGGA	3250	0.0	35.930767	4
>>END_MODULE