FastQCFastQC Report
Thu 26 May 2016
SRR1547404_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547404_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1933334
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324731.6796373518491892No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA253.8917977E-545.031
CGTTTTT153700.043.5800251
CGGGTAT2150.040.8139536
GCGATAT2050.040.6097569
TAAGACG501.0822987E-940.51
TACGGGA5400.040.4166644
AACACGT10600.040.3301941
CGTTAGG2250.040.02
CGGTCTA850.039.70588331
TGTTACG403.4598452E-739.3751
CAGTACG403.4598452E-739.3751
CACGACC11700.039.03846427
TATTGCG1500.039.01
CGACCAA11600.038.9870729
TCGATAG1100.038.8636361
TATGACG1100.038.8636361
ACACGAC11800.038.5169526
GTTTTTT177800.038.4322282
GGGCGAT35800.038.4008377
CGTAAGG3350.038.283582
Produced by FastQC (version 0.11.5)