FastQCFastQC Report
Thu 26 May 2016
SRR1547403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547403_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2397615
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163680.6826784116715987No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG207.0348225E-445.01
CTTAACG603.6379788E-1241.2499961
CGTTTTT82550.040.966081
GGGCGAT36550.040.13687
TACGGGA7250.040.034484
TCGTTGA4650.039.6774224
TCGTAAG1250.039.61
GGCGATA7900.039.30388
TTAATCG4650.039.1935520
TAATCGT4750.038.3684221
AACGGGA7750.038.3225824
TAGGGAC24000.038.255
AGGGCGA20100.037.947766
GCGGGAT13650.037.5824175
GACCGAT14750.037.525429
GTCGACA301.14029186E-437.49999633
TTAGGGA28950.037.4611434
CGTAAGG5050.037.4257432
TCGTCCC6100.037.254138
ATTGGGA19850.036.9521454
Produced by FastQC (version 0.11.5)