##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547403_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2397615
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.280617613753666	31.0	31.0	33.0	30.0	34.0
2	31.680414078156836	31.0	31.0	34.0	30.0	34.0
3	31.797718149077312	31.0	31.0	34.0	30.0	34.0
4	35.57885023241847	37.0	35.0	37.0	33.0	37.0
5	35.465335343664435	37.0	35.0	37.0	33.0	37.0
6	35.4937560867779	37.0	35.0	37.0	33.0	37.0
7	35.89254488314429	37.0	35.0	37.0	35.0	37.0
8	35.88312218600568	37.0	35.0	37.0	35.0	37.0
9	37.62393461836033	39.0	37.0	39.0	35.0	39.0
10	36.91489250776292	39.0	37.0	39.0	32.0	39.0
11	36.573110361755326	39.0	35.0	39.0	32.0	39.0
12	35.97200217716355	37.0	35.0	39.0	31.0	39.0
13	35.67859727270642	38.0	35.0	39.0	30.0	39.0
14	36.671374261505704	39.0	35.0	41.0	31.0	41.0
15	36.903162934833155	39.0	35.0	41.0	31.0	41.0
16	37.03435956148089	39.0	35.0	41.0	32.0	41.0
17	36.950138783749686	38.0	35.0	41.0	31.0	41.0
18	36.827109022924866	38.0	35.0	40.0	31.0	41.0
19	36.77642365433984	38.0	35.0	40.0	31.0	41.0
20	36.64306237657005	38.0	35.0	40.0	31.0	41.0
21	36.49724246803594	38.0	35.0	40.0	31.0	41.0
22	36.40223054994234	38.0	35.0	40.0	30.0	41.0
23	36.326132010351955	38.0	35.0	40.0	30.0	41.0
24	36.20966085047016	38.0	35.0	40.0	30.0	41.0
25	36.09943130986418	38.0	35.0	40.0	30.0	41.0
26	35.857205180981936	38.0	34.0	40.0	30.0	41.0
27	35.85066659993368	38.0	34.0	40.0	30.0	41.0
28	35.90644327800752	38.0	34.0	40.0	30.0	41.0
29	35.94336121520761	38.0	35.0	40.0	30.0	41.0
30	35.93871493129631	38.0	35.0	40.0	30.0	41.0
31	35.845605320287035	38.0	34.0	40.0	30.0	41.0
32	35.69378277997093	38.0	34.0	40.0	29.0	41.0
33	35.53281198190702	38.0	34.0	40.0	28.0	41.0
34	35.33630336813876	38.0	34.0	40.0	27.0	41.0
35	35.20446151696582	38.0	34.0	40.0	27.0	41.0
36	34.977573964126854	37.0	34.0	40.0	25.0	41.0
37	34.930685702249946	37.0	34.0	40.0	25.0	41.0
38	34.84730534301796	37.0	34.0	40.0	25.0	41.0
39	34.7128755033648	37.0	33.0	40.0	24.0	41.0
40	34.58355741017636	37.0	33.0	40.0	24.0	41.0
41	34.567583619555265	37.0	33.0	40.0	24.0	41.0
42	34.48144551981866	36.0	33.0	40.0	24.0	41.0
43	34.36756109717365	36.0	33.0	40.0	24.0	41.0
44	34.26796128652849	36.0	33.0	40.0	23.0	41.0
45	34.11332887056513	36.0	33.0	40.0	23.0	41.0
46	34.041444518823916	36.0	33.0	40.0	23.0	41.0
47	34.00338836718989	35.0	33.0	40.0	23.0	41.0
48	33.8854490816916	35.0	33.0	40.0	23.0	41.0
49	33.75408729091201	35.0	33.0	40.0	23.0	41.0
50	33.615212200457535	35.0	33.0	39.0	23.0	41.0
51	33.14081910565291	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	4.0
13	13.0
14	35.0
15	118.0
16	311.0
17	678.0
18	1449.0
19	2683.0
20	4676.0
21	7192.0
22	10660.0
23	15124.0
24	21053.0
25	28714.0
26	38904.0
27	46847.0
28	53175.0
29	60293.0
30	70835.0
31	83804.0
32	103120.0
33	131489.0
34	208346.0
35	268607.0
36	191311.0
37	237477.0
38	328676.0
39	481885.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.99061775973207	23.90750808616062	28.95940340713584	14.142470746971469
2	31.092398070582643	25.534458201170747	30.024461808922613	13.348681919323996
3	29.221664028628446	25.60340171378641	31.37855744145745	13.796376816127692
4	25.88209533223641	28.671534003582728	30.69587902978585	14.750491634395013
5	23.385030540766554	32.72431145117127	29.61693182600209	14.27372618206009
6	23.17469652133474	41.44155754781314	25.847269056958687	9.536476873893433
7	90.39754088959236	2.66348016674904	5.9613407490360215	0.9776381946225728
8	90.57271496883361	2.2231676061419368	5.8624925186070325	1.341624906417419
9	85.5900968253869	4.809612886138933	7.348844580969005	2.2514457075051664
10	45.62700850636987	31.090521205447914	12.997833263472241	10.284637024709973
11	37.725865078421684	23.342738513064024	23.414768426123462	15.516627982390835
12	35.228716870723616	20.559806307518098	26.529905760516183	17.68157106124211
13	26.030117429195265	28.386292211218233	27.818102572765017	17.765487786821488
14	20.84137778584135	29.497896868346253	29.536768830692168	20.123956515120234
15	19.695447350804862	24.008066349267917	38.18949247481351	18.106993825113708
16	24.142992098397784	22.46782740348221	35.4842207777312	17.904959720388803
17	24.39165587469214	22.588572393816357	27.736688334031946	25.28308339745956
18	24.559948115106053	23.433203412557894	31.226281116859877	20.78056735547617
19	27.886211923098582	24.938240710038933	26.47309930910509	20.702448057757397
20	29.246730605205588	25.453627876035146	26.776317298648866	18.5233242201104
21	27.0560953280656	25.88330486754546	28.43288017467358	18.627719629715365
22	26.5539713423548	23.615301038740583	28.016716612133308	21.81401100677131
23	24.49692715469331	26.16913057350742	27.795163110007238	21.538779161792032
24	24.16943504274039	23.07793369661101	32.94861768882827	19.80401357182033
25	23.438959132304394	25.122590574383292	30.407008631494215	21.0314416618181
26	21.800831242714114	29.00119493746911	27.216504734913656	21.98146908490312
27	21.704777455930163	28.274097384275628	28.815969202728546	21.205155957065667
28	20.2127113819358	27.123787597258108	32.23211399661747	20.43138702418862
29	21.559341262045827	24.75585112705751	31.779872915376323	21.90493469552034
30	22.808540987606435	26.26743659845305	30.297900204995383	20.626122208945137
31	26.43485296847075	25.328503533719964	27.586539123253733	20.650104374555546
32	27.911987537615506	25.436152176225125	26.869952014814725	19.781908271344648
33	26.58003891367046	26.192445409292155	26.743242764163554	20.484272912873834
34	22.335988054796122	26.465842097250807	29.06096266498166	22.137207182971412
35	22.622355966241454	26.608984344859373	29.37619259138769	21.392467097511485
36	26.808140589711027	26.860525981026978	25.752716762282517	20.578616666979478
37	23.30699465927599	29.235302581940804	27.28457237713311	20.173130381650097
38	23.728330027965292	29.019588215789437	25.59335005828709	21.65873169795818
39	23.16410266035206	28.42670737378603	27.048963240553636	21.360226725308276
40	25.27132170928193	25.534124536257906	26.38868208615645	22.80587166830371
41	20.814184095444848	25.205923386365196	28.182256117016287	25.79763640117367
42	22.5460718255433	26.154407609228336	26.567401355096624	24.732119210131735
43	22.24719147986645	25.94415700602474	27.695063636155098	24.113587877953716
44	21.80633671377598	26.691900075700225	28.001284609914435	23.500478600609355
45	21.326609985339598	29.049534641716875	26.25229655303291	23.37155881991062
46	22.91931773866947	28.39354942307251	26.46196324263904	22.22516959561898
47	21.83277965811859	27.12057607247202	28.258081468459284	22.78856280095011
48	23.04473403778338	25.203170650834267	29.726373917413763	22.02572139396859
49	23.041731053567815	24.589352335550117	30.096783678780785	22.272132932101275
50	22.03564792512559	28.009375984050816	28.024390905128637	21.93058518569495
51	20.96345743582685	28.685130848780975	26.93547546207377	23.4159362533184
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1215.0
1	2509.5
2	3804.0
3	15262.5
4	26721.0
5	18202.5
6	9684.0
7	9785.0
8	9886.0
9	10426.5
10	10967.0
11	11156.5
12	11346.0
13	11281.5
14	11217.0
15	10848.0
16	10479.0
17	10080.0
18	9681.0
19	10061.5
20	10442.0
21	10014.0
22	9586.0
23	10761.5
24	11937.0
25	14451.0
26	20180.0
27	23395.0
28	25853.0
29	28311.0
30	33514.0
31	38717.0
32	43178.5
33	47640.0
34	55724.5
35	63809.0
36	69280.5
37	74752.0
38	83390.5
39	92029.0
40	108253.0
41	124477.0
42	141765.5
43	159054.0
44	163236.0
45	167418.0
46	168311.0
47	169204.0
48	165524.0
49	161844.0
50	161090.5
51	160337.0
52	152640.5
53	144944.0
54	138147.0
55	131350.0
56	127262.0
57	123174.0
58	121487.0
59	119800.0
60	115371.5
61	110943.0
62	99340.5
63	87738.0
64	77849.0
65	67960.0
66	57787.5
67	47615.0
68	41138.0
69	34661.0
70	29400.5
71	24140.0
72	20208.5
73	16277.0
74	13654.5
75	8882.0
76	6732.0
77	5116.5
78	3501.0
79	2425.5
80	1350.0
81	1055.5
82	761.0
83	523.5
84	286.0
85	271.0
86	256.0
87	181.5
88	107.0
89	74.0
90	41.0
91	30.0
92	19.0
93	13.5
94	8.0
95	5.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2397615.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.176021518201622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.38087336687066	16.419422522200236
2	5.61978994537822	2.267700057314482
3	1.809869155365488	1.0954787707135043
4	0.9608371216363327	0.7754348176648624
5	0.6261580576669065	0.6316689222641418
6	0.4335197175249178	0.5248021889608459
7	0.3571601860323149	0.5044250119183017
8	0.3195935645035741	0.5158501307602297
9	0.2751630158997797	0.4996525436826489
>10	5.335691545073959	28.57456979349784
>50	2.3203026096961112	33.11368270995458
>100	0.5591646244960787	14.059055170714712
>500	0.0012513932371060082	0.18123954256727193
>1k	4.1713107903533606E-4	0.14824864371128374
>5k	0.0	0.0
>10k+	2.0856553951766803E-4	0.688769174075019
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16368	0.6826784116715987	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.170811410505857E-5	0.0	0.0	0.01689178621254872	0.0
2	4.170811410505857E-5	0.0	0.0	0.059267230143288226	0.0
3	4.170811410505857E-5	0.0	0.0	0.08312427141138173	0.0
4	4.170811410505857E-5	0.0	0.0	0.14501911274328863	0.0
5	4.170811410505857E-5	0.0	0.0	0.2555873232357989	0.0
6	4.170811410505857E-5	0.0	0.0	0.43109506738988534	0.0
7	4.170811410505857E-5	0.0	0.0	0.5234785401325901	0.0
8	1.251243423151757E-4	0.0	0.0	0.7130002106259762	0.0
9	1.251243423151757E-4	0.0	0.0	0.7905355947472801	0.0
10	1.251243423151757E-4	0.0	0.0	0.9089449306915414	0.0
11	1.251243423151757E-4	0.0	0.0	1.062472498712262	0.0
12	1.251243423151757E-4	0.0	0.0	1.1804647535154726	0.0
13	1.251243423151757E-4	0.0	0.0	1.2429852165589554	0.0
14	1.251243423151757E-4	0.0	0.0	1.2770190376686832	0.0
15	1.251243423151757E-4	0.0	0.0	1.3135970537388195	0.0
16	1.251243423151757E-4	0.0	0.0	1.379370749682497	0.0
17	1.251243423151757E-4	0.0	0.0	1.452818738621505	0.0
18	1.6683245642023427E-4	0.0	0.0	1.5450353789077895	0.0
19	1.6683245642023427E-4	0.0	0.0	1.6078895068641128	0.0
20	1.6683245642023427E-4	0.0	0.0	1.6800028361517592	0.0
21	1.6683245642023427E-4	0.0	0.0	1.7687577029673238	0.0
22	1.6683245642023427E-4	0.0	0.0	1.8708174581824022	0.0
23	1.6683245642023427E-4	0.0	0.0	1.9799675927953404	0.0
24	1.6683245642023427E-4	0.0	0.0	2.0690561245237453	0.0
25	1.6683245642023427E-4	0.0	0.0	2.152096979706917	0.0
26	1.6683245642023427E-4	0.0	0.0	2.2334695103258864	0.0
27	1.6683245642023427E-4	0.0	0.0	2.3205560525772486	0.0
28	1.6683245642023427E-4	0.0	0.0	2.404723026841257	0.0
29	1.6683245642023427E-4	0.0	0.0	2.5020280570483586	0.0
30	1.6683245642023427E-4	0.0	0.0	2.6416668230720948	0.0
31	1.6683245642023427E-4	0.0	0.0	2.7563224287469006	0.0
32	1.6683245642023427E-4	0.0	0.0	2.8586324326466093	0.0
33	1.6683245642023427E-4	0.0	0.0	2.960900728432213	0.0
34	1.6683245642023427E-4	0.0	0.0	3.077182950557116	0.0
35	1.6683245642023427E-4	0.0	0.0	3.206603228625113	0.0
36	1.6683245642023427E-4	0.0	0.0	3.3229271588641214	0.0
37	1.6683245642023427E-4	0.0	0.0	3.4490942040319235	0.0
38	1.6683245642023427E-4	0.0	0.0	3.594738938486788	0.0
39	2.0854057052529284E-4	0.0	0.0	3.821088873734941	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCG	20	7.0348225E-4	45.0	1
CTTAACG	60	3.6379788E-12	41.249996	1
CGTTTTT	8255	0.0	40.96608	1
GGGCGAT	3655	0.0	40.1368	7
TACGGGA	725	0.0	40.03448	4
TCGTTGA	465	0.0	39.67742	24
TCGTAAG	125	0.0	39.6	1
GGCGATA	790	0.0	39.3038	8
TTAATCG	465	0.0	39.19355	20
TAATCGT	475	0.0	38.36842	21
AACGGGA	775	0.0	38.322582	4
TAGGGAC	2400	0.0	38.25	5
AGGGCGA	2010	0.0	37.94776	6
GCGGGAT	1365	0.0	37.582417	5
GACCGAT	1475	0.0	37.52542	9
GTCGACA	30	1.14029186E-4	37.499996	33
TTAGGGA	2895	0.0	37.461143	4
CGTAAGG	505	0.0	37.425743	2
TCGTCCC	610	0.0	37.2541	38
ATTGGGA	1985	0.0	36.952145	4
>>END_MODULE