FastQCFastQC Report
Thu 26 May 2016
SRR1547402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547402_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3588124
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT605011.6861457407826483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT298500.043.3793951
TACGGGA10900.040.8715634
TACGTCG403.4609548E-739.3750041
ACGGGAC8800.038.863645
AGGGCGA33850.038.8183176
GGGCGAT68550.038.3698047
GACCGAT21350.038.149889
CACGACC12500.037.9827
TAGGGCG13000.037.730775
GTTTTTT350700.037.6860542
TGGGCGA19600.037.5382656
ACGGGTA6250.037.445
ATAGGGT25850.037.3404274
CGGGTAT3750.037.26
CGTTAGG5100.037.0588232
CGAGGGA22850.037.024074
CGACCAA12850.036.94552229
TAGGGTC18900.036.9047625
TAGGGAT44350.036.832025
ACGACCA12950.036.48648528
Produced by FastQC (version 0.11.5)