##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547402_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3588124
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30377517610874	31.0	31.0	33.0	30.0	34.0
2	31.706748986378397	31.0	31.0	34.0	30.0	34.0
3	31.729182157584297	31.0	31.0	34.0	30.0	34.0
4	35.56467474368221	37.0	35.0	37.0	33.0	37.0
5	35.4902492221562	37.0	35.0	37.0	33.0	37.0
6	35.53340129828289	37.0	35.0	37.0	33.0	37.0
7	35.93878667515393	37.0	35.0	37.0	35.0	37.0
8	35.946620295173744	37.0	35.0	37.0	35.0	37.0
9	37.69848227095831	39.0	37.0	39.0	35.0	39.0
10	36.87267859193272	39.0	37.0	39.0	32.0	39.0
11	36.67666752876991	39.0	35.0	39.0	32.0	39.0
12	36.43600026086055	39.0	35.0	39.0	32.0	39.0
13	36.329233605081654	39.0	35.0	39.0	32.0	39.0
14	37.41891640311204	39.0	36.0	41.0	32.0	41.0
15	37.53118537709399	40.0	36.0	41.0	32.0	41.0
16	37.59633111899143	40.0	36.0	41.0	32.0	41.0
17	37.49937125918725	39.0	36.0	41.0	32.0	41.0
18	37.32391327612981	39.0	36.0	41.0	32.0	41.0
19	37.19816121181988	39.0	36.0	41.0	32.0	41.0
20	37.00157575379223	39.0	35.0	41.0	32.0	41.0
21	36.880617838179504	38.0	35.0	41.0	31.0	41.0
22	36.79492263923989	38.0	35.0	40.0	31.0	41.0
23	36.686733513111584	38.0	35.0	40.0	31.0	41.0
24	36.57972857125339	38.0	35.0	40.0	31.0	41.0
25	36.50527211434164	38.0	35.0	40.0	31.0	41.0
26	36.305985523354266	38.0	35.0	40.0	30.0	41.0
27	36.28539342564527	38.0	35.0	40.0	30.0	41.0
28	36.30175322814931	38.0	35.0	40.0	30.0	41.0
29	36.29413838540697	38.0	35.0	40.0	30.0	41.0
30	36.23709855066324	38.0	35.0	40.0	30.0	41.0
31	36.12845682033285	38.0	35.0	40.0	30.0	41.0
32	35.883709983267025	38.0	35.0	40.0	29.0	41.0
33	35.593746760145414	38.0	34.0	40.0	27.0	41.0
34	35.2671008582758	38.0	34.0	40.0	25.0	41.0
35	35.04052479791668	38.0	34.0	40.0	24.0	41.0
36	34.802760997111584	38.0	34.0	40.0	23.0	41.0
37	34.755979726453155	38.0	34.0	40.0	23.0	41.0
38	34.660930057043736	38.0	34.0	40.0	23.0	41.0
39	34.528794712780275	38.0	33.0	40.0	23.0	41.0
40	34.42222648938554	38.0	33.0	40.0	22.0	41.0
41	34.38043501283679	38.0	33.0	40.0	22.0	41.0
42	34.2919614818217	37.0	33.0	40.0	22.0	41.0
43	34.17853312761766	37.0	33.0	40.0	21.0	41.0
44	34.08883890300335	37.0	33.0	40.0	21.0	41.0
45	33.94823646005545	37.0	33.0	40.0	20.0	41.0
46	33.87191774866197	37.0	33.0	40.0	20.0	41.0
47	33.833360552756815	37.0	33.0	40.0	20.0	41.0
48	33.70402332806781	36.0	33.0	40.0	20.0	41.0
49	33.576520488143665	36.0	33.0	40.0	20.0	41.0
50	33.472158152839754	36.0	32.0	40.0	20.0	41.0
51	33.036001821564696	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	3.0
13	10.0
14	42.0
15	120.0
16	321.0
17	798.0
18	1735.0
19	3514.0
20	5922.0
21	9602.0
22	14808.0
23	22062.0
24	32664.0
25	49897.0
26	71388.0
27	84107.0
28	86447.0
29	89315.0
30	98570.0
31	114561.0
32	138287.0
33	173710.0
34	254904.0
35	329469.0
36	304402.0
37	370137.0
38	520666.0
39	810447.0
40	212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.335490077823398	23.410172000744677	27.684355390170463	18.569982531261463
2	34.68740210761947	24.467298231610723	27.874287510688035	12.971012150081771
3	26.797122953387344	24.68936413568762	35.40808511634492	13.105427794580121
4	23.350530806627642	27.341390654280623	35.134209408593456	14.173869130498277
5	21.820204652904973	31.20469080778702	33.38298787890274	13.59211666040527
6	21.03399993980141	39.32860179860005	29.796183186534243	9.841215075064296
7	86.49274662748556	2.2389137053234505	10.136857031696787	1.1314826354942025
8	87.16928400467765	1.6442296865994597	9.98923114139868	1.1972551673242062
9	82.41092002394566	3.8092607724816645	11.746723357386756	2.033095846185918
10	34.2007411115112	30.25271701869835	24.434774272015126	11.111767597775328
11	28.437757446509654	25.138540362596164	29.65608211979296	16.767620071101224
12	27.380352518474837	23.254965547455996	31.136214913419934	18.22846702064923
13	23.14044888080791	25.58470666008198	32.74649928486307	18.528345174247043
14	19.98699041616176	27.653670831888753	33.06747481413686	19.29186393781263
15	19.61498543528596	25.79498367391985	37.12706138360882	17.46296950718537
16	22.88900829514253	24.57429007470199	35.77320627715207	16.763495353003407
17	22.22175153367052	24.83899664560088	32.20850227026714	20.730749550461468
18	22.54506811916199	25.25559317348007	33.215184313585596	18.984154393772346
19	23.75767950048549	27.149563393015402	30.695148774122632	18.39760833237647
20	25.709981037444635	26.868051382839614	30.416312256767046	17.005655322948705
21	24.473513178474324	26.342651480272146	32.17048797644674	17.01334736480679
22	23.509388192827227	24.090555398865813	32.19398214777416	20.2060742605328
23	21.616170455647577	26.709528433242554	32.26053503167672	19.413766079433152
24	20.677100345473008	26.736394840312094	34.60914394262852	17.977360871586377
25	21.313003675458262	27.42349484020062	32.148275812095676	19.11522567224544
26	20.332324077986154	29.658283827426253	30.70699340379541	19.30239869079218
27	19.73025458428973	28.93656963917635	32.09788736398185	19.235288412552073
28	18.61005918413076	28.671472892241184	33.80390421289788	18.914563710730175
29	19.917065296517066	26.783940577304467	33.56015566909059	19.73883845708788
30	20.908446865269987	27.37154568794167	33.06393536009347	18.656072086694884
31	22.76050103062213	26.94820468857821	31.04143000632085	19.24986427447881
32	23.31142959384904	27.3615125898659	30.555354274267	18.771703542018056
33	22.476229918475504	27.531239165647563	30.152441777374474	19.84008913850246
34	20.26262191607648	27.94318145080828	31.85734383761542	19.93685279549982
35	20.069596256985545	28.359359932934314	30.95333383127227	20.617709978807866
36	22.07515682289687	29.37457010961717	28.915137826897848	19.635135240588117
37	20.940942955148707	29.51213503212264	29.636907754581504	19.910014258147154
38	20.839134879396585	29.917444324666594	28.96009168022064	20.283329115716178
39	20.653355346693704	29.675702400474453	28.716120178678327	20.954822074153512
40	22.17016468773097	28.14192040185902	29.080433117696042	20.607481792713962
41	19.796473031589766	27.914252684689828	29.690696308154347	22.598577975566062
42	20.6983092000165	28.716231657545837	28.51869110432081	22.066768038116855
43	20.66308187788382	28.321401378547677	29.318050323790374	21.697466419778134
44	20.475853119903327	29.290292085780763	29.117471971425736	21.116382822890177
45	20.081385147224566	29.858555612905242	28.2428087769542	21.817250462915997
46	20.91070431233703	29.38387859505413	28.503251281170883	21.202165811437954
47	20.502858875557255	28.89888978195848	29.594044130024493	21.004207212459768
48	20.896184189844053	27.86369701827473	30.44334588213785	20.796772909743364
49	20.640368058628965	27.99493551504909	30.233904959806296	21.13079146651565
50	20.08339176683972	28.88236303985035	30.17451459314115	20.85973060016878
51	19.539235544813945	29.09768447244298	29.2967021206625	22.066377862080575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1416.0
1	2491.5
2	3567.0
3	46309.0
4	89051.0
5	58820.0
6	28589.0
7	28743.0
8	28897.0
9	30870.5
10	32844.0
11	34654.0
12	36464.0
13	36657.5
14	36851.0
15	35524.0
16	34197.0
17	32298.0
18	30399.0
19	28843.0
20	27287.0
21	27087.5
22	26888.0
23	27698.5
24	28509.0
25	30459.5
26	36772.5
27	41135.0
28	47592.5
29	54050.0
30	60190.0
31	66330.0
32	77334.0
33	88338.0
34	99010.0
35	109682.0
36	121120.0
37	132558.0
38	145539.0
39	158520.0
40	173121.5
41	187723.0
42	205161.0
43	222599.0
44	231740.0
45	240881.0
46	249907.0
47	258933.0
48	262213.5
49	265494.0
50	258866.0
51	252238.0
52	233152.0
53	214066.0
54	199031.0
55	183996.0
56	172996.0
57	161996.0
58	150786.5
59	139577.0
60	127875.5
61	116174.0
62	101475.5
63	86777.0
64	73254.5
65	59732.0
66	49775.0
67	39818.0
68	32223.0
69	24628.0
70	19956.0
71	15284.0
72	13400.0
73	11516.0
74	10183.5
75	7235.5
76	5620.0
77	3855.5
78	2091.0
79	1542.0
80	993.0
81	848.0
82	703.0
83	454.5
84	206.0
85	160.5
86	115.0
87	93.0
88	71.0
89	58.5
90	46.0
91	28.0
92	10.0
93	6.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3588124.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.903849693201078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.19892765526319	18.692554128409164
2	6.879893475627248	3.28911879093257
3	2.7211566605909137	1.951383594192545
4	1.496864328218313	1.4312327965137983
5	1.0195950002499632	1.2186122816957217
6	0.7321664060161578	1.0500957431892883
7	0.5582390262448018	0.9340843243364273
8	0.463429592359524	0.8862201055314807
9	0.37968580402340474	0.81683571510159
>10	5.72719300258882	33.71267104506514
>50	1.5547188032376587	25.629095033313053
>100	0.2653001573750992	8.053353898249139
>500	0.0017688051281177192	0.28272063783229284
>1k	9.433627349958396E-4	0.346649890733871
>5k	0.0	0.0
>10k+	1.1792034187447995E-4	1.7053720149040437
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60501	1.6861457407826483	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.786971687712019E-5	0.0	0.0	0.04592929341349407	0.0
2	2.786971687712019E-5	0.0	0.0	0.11749872635393871	0.0
3	2.786971687712019E-5	0.0	0.0	0.20386697895613418	0.0
4	2.786971687712019E-5	0.0	0.0	0.29809449171767755	0.0
5	2.786971687712019E-5	0.0	0.0	0.5035221748189305	0.0
6	2.786971687712019E-5	0.0	0.0	0.9478212012739805	0.0
7	2.786971687712019E-5	0.0	0.0	1.2077620505868805	0.0
8	2.786971687712019E-5	0.0	0.0	1.6161927514210768	0.0
9	2.786971687712019E-5	0.0	0.0	1.8721761009374258	0.0
10	2.786971687712019E-5	0.0	0.0	2.174423180469794	0.0
11	2.786971687712019E-5	0.0	0.0	2.475806298778972	0.0
12	2.786971687712019E-5	0.0	0.0	2.7086020438535567	0.0
13	2.786971687712019E-5	0.0	0.0	2.8782728802014645	0.0
14	2.786971687712019E-5	0.0	0.0	2.99986845493634	0.0
15	2.786971687712019E-5	0.0	0.0	3.1010633969171635	0.0
16	2.786971687712019E-5	0.0	0.0	3.2306297106788953	0.0
17	2.786971687712019E-5	0.0	0.0	3.371566868926492	0.0
18	2.786971687712019E-5	0.0	0.0	3.524376526563742	0.0
19	2.786971687712019E-5	0.0	0.0	3.643268738761537	0.0
20	2.786971687712019E-5	0.0	0.0	3.762216690393086	0.0
21	2.786971687712019E-5	0.0	0.0	3.901175098742407	0.0
22	2.786971687712019E-5	0.0	0.0	4.05008299601686	0.0
23	2.786971687712019E-5	0.0	0.0	4.215629114266954	0.0
24	2.786971687712019E-5	0.0	0.0	4.357569582322127	0.0
25	2.786971687712019E-5	0.0	0.0	4.491344223332304	0.0
26	2.786971687712019E-5	0.0	0.0	4.610793829867641	0.0
27	2.786971687712019E-5	0.0	0.0	4.741279844286318	0.0
28	2.786971687712019E-5	0.0	0.0	4.876754538025999	0.0
29	2.786971687712019E-5	0.0	0.0	5.0239066431371935	0.0
30	2.786971687712019E-5	0.0	0.0	5.195166053347097	0.0
31	2.786971687712019E-5	0.0	0.0	5.352713562853458	0.0
32	2.786971687712019E-5	0.0	0.0	5.530633835396993	0.0
33	2.786971687712019E-5	2.786971687712019E-5	0.0	5.683554971901751	0.0
34	2.786971687712019E-5	2.786971687712019E-5	0.0	5.845310808656557	0.0
35	2.786971687712019E-5	2.786971687712019E-5	0.0	6.010968405774159	0.0
36	2.786971687712019E-5	2.786971687712019E-5	0.0	6.175929259969834	0.0
37	2.786971687712019E-5	2.786971687712019E-5	0.0	6.340695026147368	0.0
38	2.786971687712019E-5	2.786971687712019E-5	0.0	6.528063132712248	0.0
39	2.786971687712019E-5	2.786971687712019E-5	0.0	6.807986569026043	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	29850	0.0	43.379395	1
TACGGGA	1090	0.0	40.871563	4
TACGTCG	40	3.4609548E-7	39.375004	1
ACGGGAC	880	0.0	38.86364	5
AGGGCGA	3385	0.0	38.818317	6
GGGCGAT	6855	0.0	38.369804	7
GACCGAT	2135	0.0	38.14988	9
CACGACC	1250	0.0	37.98	27
TAGGGCG	1300	0.0	37.73077	5
GTTTTTT	35070	0.0	37.686054	2
TGGGCGA	1960	0.0	37.538265	6
ACGGGTA	625	0.0	37.44	5
ATAGGGT	2585	0.0	37.340427	4
CGGGTAT	375	0.0	37.2	6
CGTTAGG	510	0.0	37.058823	2
CGAGGGA	2285	0.0	37.02407	4
CGACCAA	1285	0.0	36.945522	29
TAGGGTC	1890	0.0	36.904762	5
TAGGGAT	4435	0.0	36.83202	5
ACGACCA	1295	0.0	36.486485	28
>>END_MODULE