FastQCFastQC Report
Thu 26 May 2016
SRR1547401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547401_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3352100
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248660.7418036454759703No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCG253.892411E-545.00000424
CCGTCGA253.892411E-545.00000417
CGGTCGA351.2128658E-745.0000046
TATCCGA302.166751E-645.00000444
ATACTCG1250.043.244
TTTCGCG1100.042.9545481
CGTTTTT167350.042.9429361
CGTTAGG4300.040.8139572
TACGGGA9250.040.1351364
CAATCGT451.9301297E-840.00000419
GACCGAT22150.039.8194129
TAGTACG1250.039.6000021
TATCTCG403.4608638E-739.37500419
AGGGCGA30600.038.8235326
CGTATTA356.251872E-638.5714342
TTAGCGA356.251872E-638.5714329
TACCTCG356.251872E-638.571431
CCGAACG700.038.571431
ATAGGGT23650.038.5306554
TAGGGTC19850.038.425695
Produced by FastQC (version 0.11.5)