##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547401_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3352100
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25696697592554	31.0	31.0	33.0	30.0	34.0
2	31.660701948032578	31.0	31.0	34.0	30.0	34.0
3	31.747276632558695	31.0	31.0	34.0	30.0	34.0
4	35.542027385817846	37.0	35.0	37.0	33.0	37.0
5	35.44197189821306	37.0	35.0	37.0	33.0	37.0
6	35.47225142448018	37.0	35.0	37.0	33.0	37.0
7	35.91360490438829	37.0	35.0	37.0	35.0	37.0
8	35.9172259180812	37.0	35.0	37.0	35.0	37.0
9	37.6790895259688	39.0	37.0	39.0	35.0	39.0
10	36.752748426359595	39.0	37.0	39.0	32.0	39.0
11	36.54124578622356	39.0	35.0	39.0	32.0	39.0
12	36.01031771128546	37.0	35.0	39.0	31.0	39.0
13	35.706678500044745	38.0	35.0	39.0	30.0	39.0
14	36.69232809283733	39.0	35.0	41.0	31.0	41.0
15	36.959147400137226	39.0	35.0	41.0	31.0	41.0
16	37.1097595537126	39.0	35.0	41.0	32.0	41.0
17	37.050648548670985	39.0	35.0	41.0	32.0	41.0
18	36.94206288595209	38.0	35.0	40.0	32.0	41.0
19	36.86107813012738	38.0	35.0	40.0	31.0	41.0
20	36.677023656812146	38.0	35.0	40.0	31.0	41.0
21	36.54420244026133	38.0	35.0	40.0	31.0	41.0
22	36.44502819128308	38.0	35.0	40.0	31.0	41.0
23	36.389138152203095	38.0	35.0	40.0	31.0	41.0
24	36.277367620297724	38.0	35.0	40.0	31.0	41.0
25	36.176814832493065	38.0	35.0	40.0	30.0	41.0
26	35.96749380985054	38.0	34.0	40.0	30.0	41.0
27	35.94299483905611	38.0	34.0	40.0	30.0	41.0
28	36.02080695683303	38.0	35.0	40.0	30.0	41.0
29	36.07501327526028	38.0	35.0	40.0	30.0	41.0
30	36.06962680110975	38.0	35.0	40.0	30.0	41.0
31	35.93893797917723	38.0	35.0	40.0	30.0	41.0
32	35.73422272605232	38.0	34.0	40.0	29.0	41.0
33	35.52172250231198	38.0	34.0	40.0	28.0	41.0
34	35.302610602309	38.0	34.0	40.0	27.0	41.0
35	35.1546970555771	38.0	34.0	40.0	26.0	41.0
36	34.90866978908744	38.0	34.0	40.0	25.0	41.0
37	34.8441755914203	38.0	34.0	40.0	24.0	41.0
38	34.78600578741684	37.0	34.0	40.0	24.0	41.0
39	34.68114346230721	37.0	34.0	40.0	24.0	41.0
40	34.54782971868381	37.0	33.0	40.0	23.0	41.0
41	34.55457504251066	37.0	34.0	40.0	23.0	41.0
42	34.46753766295755	37.0	33.0	40.0	23.0	41.0
43	34.36801378240506	37.0	33.0	40.0	23.0	41.0
44	34.27188150711494	37.0	33.0	40.0	23.0	41.0
45	34.10832940544733	37.0	33.0	40.0	23.0	41.0
46	34.04829629187673	36.0	33.0	40.0	23.0	41.0
47	34.023522567942486	36.0	33.0	40.0	23.0	41.0
48	33.946835416604515	36.0	33.0	40.0	23.0	41.0
49	33.84175293099848	36.0	33.0	40.0	23.0	41.0
50	33.70762059604427	36.0	33.0	40.0	22.0	41.0
51	33.24822201008323	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	1.0
11	3.0
12	1.0
13	17.0
14	29.0
15	100.0
16	314.0
17	848.0
18	1878.0
19	3565.0
20	6029.0
21	9645.0
22	14439.0
23	21166.0
24	30344.0
25	43694.0
26	60700.0
27	70825.0
28	76464.0
29	83297.0
30	95715.0
31	113683.0
32	139196.0
33	176198.0
34	279975.0
35	384743.0
36	248734.0
37	307455.0
38	456525.0
39	726319.0
40	195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.307777214283583	24.862414605769516	30.188120879448704	15.641687300498194
2	30.86685958056144	25.413949464514786	30.21022642522598	13.508964529697801
3	27.230124399630085	25.193281823334623	33.69156648071358	13.88502729632171
4	23.911309328480655	28.05617374183348	33.69663792846275	14.335879001223114
5	21.8448733629665	32.32176844366218	32.17329435279377	13.66006384057755
6	20.948987202052443	41.72718594314012	28.029921541720114	9.293905313087318
7	89.56937442200412	2.7024850093970945	6.819903940813221	0.9082366277855672
8	90.29838011992483	1.8658452910116048	6.564332806300528	1.271441782763044
9	86.35729840995197	3.441305450314728	8.399182601951017	1.8022135377822859
10	40.00829330867218	25.447271859431403	23.254765669281944	11.289669162614482
11	36.062259479132486	23.113868917991706	24.628740192715014	16.195131410160794
12	34.01974881417619	22.597864025536232	26.03818501834671	17.344202141940873
13	23.503594761492796	32.13734673786581	27.209599952268725	17.14945854837266
14	18.764028519435577	34.18889651263387	28.466394200650342	18.580680767280214
15	17.86653142806002	25.502848960353212	40.023865636466695	16.60675397512007
16	19.831061125861403	23.088422183109095	39.48205602458161	17.5984606664479
17	20.412577190417945	22.882312580173622	29.86969362489186	26.83541660451657
18	22.340652128516453	24.807672802124042	32.91539631872558	19.93627875063393
19	25.99451090361266	26.131827809432895	27.830166164493896	20.04349512246055
20	27.458369380388415	26.071746069627995	28.104352495450613	18.36553205453298
21	23.407267086304106	27.45407356582441	30.733092688165627	18.405566659705855
22	23.236031144655588	24.34118910533695	29.66662689060589	22.756152859401567
23	21.200083529727635	28.440678977357475	28.69997911756809	21.6592583753468
24	20.882491572447123	25.643268398914117	35.57745890635721	17.896781122281556
25	19.86307091077235	26.20957012022314	33.91590346350049	20.01145550550401
26	19.776319322215922	31.413501983831033	28.761761283971243	20.048417409981802
27	19.180752364189612	31.74791921482056	30.083678887861343	18.987649533128486
28	16.808657259628294	29.281196861668807	35.07449061782167	18.83565526088124
29	17.562990364249277	26.226753378479163	35.0026848841025	21.207571373169056
30	20.06273679186182	28.67011723993914	31.69216312162525	19.57498284657379
31	24.377285880492824	27.396229229438262	28.984099519704067	19.242385370364843
32	25.690820679574	26.228155484621578	29.165120372303928	18.91590346350049
33	24.719071626741446	27.5555920169446	27.65749828465738	20.067838071656574
34	19.776349154261506	28.03755854538946	29.694221532770506	22.491870767578533
35	19.60394976283524	27.38531070075475	31.317323468870264	21.69341606753975
36	25.65794576534113	27.10026550520569	27.740461203424722	19.501327526028458
37	20.31058142656842	30.54356970257451	29.956534709585036	19.18931416127204
38	21.092688165627518	30.236985770114256	27.675069359505983	20.995256704752244
39	20.541421795292504	29.733003192028878	28.909101757107486	20.816473255571136
40	23.342203394886788	26.657736941022048	28.112794964350705	21.88726469974046
41	19.109483607290954	26.308015870648248	29.130515199427226	25.451985322633575
42	21.234300886011752	26.631574237045434	27.36084842337639	24.77327645356642
43	21.47394170818293	27.472300945675844	27.89511052772889	23.158646818412336
44	20.711553951254437	28.879389039706453	28.708719906924017	21.700337102115093
45	19.268965722979626	32.06676411801557	26.14644551176874	22.51782464723606
46	21.370692998418903	30.12025297574655	27.442946212821813	21.06610781301274
47	20.783061364517767	27.850392291399423	28.613943498105666	22.752602845977147
48	21.56418364607261	26.2175352763939	30.437218460069808	21.78106261746368
49	21.13206646579756	25.40479102652069	31.316995316368846	22.14614719131291
50	20.016079472569434	30.314429760448675	28.301124668118494	21.368366098863397
51	19.19545956266221	30.994898720205242	27.0613346857194	22.748307031413145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1524.0
1	2402.0
2	3280.0
3	21318.0
4	39356.0
5	27810.5
6	16265.0
7	16912.5
8	17560.0
9	19022.0
10	20484.0
11	21230.0
12	21976.0
13	21919.5
14	21863.0
15	20793.0
16	19723.0
17	18744.0
18	17765.0
19	17367.0
20	16969.0
21	17221.0
22	17473.0
23	18609.5
24	19746.0
25	20991.0
26	26480.5
27	30725.0
28	36233.0
29	41741.0
30	46292.5
31	50844.0
32	65380.0
33	79916.0
34	86086.0
35	92256.0
36	106640.5
37	121025.0
38	139511.5
39	157998.0
40	185045.0
41	212092.0
42	241022.5
43	269953.0
44	275569.0
45	281185.0
46	289839.0
47	298493.0
48	288705.0
49	278917.0
50	265091.0
51	251265.0
52	238728.0
53	226191.0
54	208215.0
55	190239.0
56	168238.0
57	146237.0
58	130633.5
59	115030.0
60	101183.0
61	87336.0
62	76102.5
63	64869.0
64	53037.5
65	41206.0
66	32677.5
67	24149.0
68	18501.0
69	12853.0
70	10196.0
71	7539.0
72	6305.5
73	5072.0
74	4402.0
75	3094.0
76	2456.0
77	1920.5
78	1385.0
79	1059.0
80	733.0
81	477.0
82	221.0
83	167.0
84	113.0
85	79.5
86	46.0
87	40.5
88	35.0
89	23.5
90	12.0
91	11.0
92	10.0
93	6.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3352100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.734945974621542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.8866159440643	16.16027941700692
2	7.4769377456241015	2.9511392493100046
3	2.4167407003120642	1.4308274146598288
4	1.1462436019867677	0.9048422223585784
5	0.6638947107080574	0.6550963124330256
6	0.47059477628624746	0.5572297491568923
7	0.3109273442657786	0.4295294038782385
8	0.2534833982242011	0.40019849355344683
9	0.19908861389435795	0.35361027354306984
>10	2.5622783296498453	12.33845238620557
>50	1.2123494307055087	17.506684201450216
>100	1.3955062820066777	44.77143036805148
>500	0.004728936869517777	0.6307888053686966
>1k	4.576390518888171E-4	0.16130226789316454
>5k	0.0	0.0
>10k+	1.5254635062960569E-4	0.7485894351309126
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24866	0.7418036454759703	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.949613675009695E-5	0.0	0.0	0.02792279466603025	0.0
2	8.949613675009695E-5	0.0	0.0	0.11867187733062856	0.0
3	8.949613675009695E-5	0.0	0.0	0.17520360371110646	0.0
4	8.949613675009695E-5	0.0	0.0	0.3064944363234987	0.0
5	8.949613675009695E-5	0.0	0.0	0.5164523731392262	0.0
6	1.1932818233346261E-4	0.0	0.0	0.7998269741356164	0.0
7	1.1932818233346261E-4	0.0	0.0	0.93228125652576	0.0
8	1.1932818233346261E-4	0.0	0.0	1.23692610602309	0.0
9	1.1932818233346261E-4	0.0	0.0	1.3274663643686047	0.0
10	1.1932818233346261E-4	0.0	0.0	1.5026998001252947	0.0
11	1.1932818233346261E-4	0.0	0.0	1.752632678022732	0.0
12	1.1932818233346261E-4	0.0	0.0	1.9380090092777662	0.0
13	1.1932818233346261E-4	0.0	0.0	2.021240416455356	0.0
14	1.1932818233346261E-4	0.0	0.0	2.060201067987232	0.0
15	1.1932818233346261E-4	0.0	0.0	2.112198323439038	0.0
16	1.1932818233346261E-4	0.0	0.0	2.236031144655589	0.0
17	1.1932818233346261E-4	0.0	0.0	2.376271590942991	0.0
18	1.1932818233346261E-4	0.0	0.0	2.541421795292503	0.0
19	1.1932818233346261E-4	0.0	0.0	2.6366456847946065	0.0
20	1.1932818233346261E-4	0.0	0.0	2.7443692013961396	0.0
21	1.1932818233346261E-4	0.0	0.0	2.8993765102473077	0.0
22	1.1932818233346261E-4	0.0	0.0	3.0683153843859072	0.0
23	1.1932818233346261E-4	0.0	0.0	3.24632320038185	0.0
24	1.1932818233346261E-4	0.0	0.0	3.385489693028251	0.0
25	1.1932818233346261E-4	0.0	0.0	3.5184511201933115	0.0
26	1.1932818233346261E-4	0.0	0.0	3.6470869007487843	0.0
27	1.4916022791682825E-4	0.0	0.0	3.7797798395035946	0.0
28	1.4916022791682825E-4	0.0	0.0	3.912950090987739	0.0
29	1.4916022791682825E-4	0.0	0.0	4.063691417320485	0.0
30	1.4916022791682825E-4	0.0	0.0	4.236448793293756	0.0
31	1.4916022791682825E-4	0.0	0.0	4.396080069210345	0.0
32	1.4916022791682825E-4	0.0	0.0	4.546075594403508	0.0
33	1.4916022791682825E-4	0.0	0.0	4.69908415620059	0.0
34	1.4916022791682825E-4	0.0	0.0	4.852570030727007	0.0
35	1.4916022791682825E-4	0.0	0.0	5.044360251782464	0.0
36	1.4916022791682825E-4	0.0	0.0	5.207899525670475	0.0
37	1.4916022791682825E-4	0.0	0.0	5.38176068733033	0.0
38	1.4916022791682825E-4	0.0	0.0	5.571253840875869	0.0
39	1.4916022791682825E-4	0.0	0.0	5.847826735479252	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATCG	25	3.892411E-5	45.000004	24
CCGTCGA	25	3.892411E-5	45.000004	17
CGGTCGA	35	1.2128658E-7	45.000004	6
TATCCGA	30	2.166751E-6	45.000004	44
ATACTCG	125	0.0	43.2	44
TTTCGCG	110	0.0	42.954548	1
CGTTTTT	16735	0.0	42.942936	1
CGTTAGG	430	0.0	40.813957	2
TACGGGA	925	0.0	40.135136	4
CAATCGT	45	1.9301297E-8	40.000004	19
GACCGAT	2215	0.0	39.819412	9
TAGTACG	125	0.0	39.600002	1
TATCTCG	40	3.4608638E-7	39.375004	19
AGGGCGA	3060	0.0	38.823532	6
CGTATTA	35	6.251872E-6	38.57143	42
TTAGCGA	35	6.251872E-6	38.57143	29
TACCTCG	35	6.251872E-6	38.57143	1
CCGAACG	70	0.0	38.57143	1
ATAGGGT	2365	0.0	38.530655	4
TAGGGTC	1985	0.0	38.42569	5
>>END_MODULE