FastQCFastQC Report
Thu 26 May 2016
SRR1547400_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547400_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2453331
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262041.0680988419418334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT143750.042.182611
CGTAAGG4250.040.7647062
CACGACC12450.040.4819327
ACGACCA12700.039.1535428
GGGCGAT43650.038.9690747
AACACGT12050.038.6514541
CGACCAA12850.038.521429
CGGGTAT3000.038.256
GGCGATA9500.038.1315778
TAGGGAT35100.038.012825
TACGGGA9900.037.9545444
TTTAGCG1850.037.70271
TAAGGGA35700.037.54
ACACGAC13300.037.2180526
AGGGCGA25150.037.127246
CGAGACA13300.037.04887422
GCGATAC1100.036.818189
TATAGCG1100.036.818181
GCGAGAC13450.036.80297521
GACACGA13400.036.77238525
Produced by FastQC (version 0.11.5)